Palmitate lipotoxicity is closely associated with the fatty acid-albumin complexes in BV-2 microglia
Palmitic acid (PA) is considered a major contributor to the inflammation in many metabolic diseases; however, this role has been questioned recently for the complicated procedures in preparing PA-bovine serum albumin (BSA) complex. This study is aimed to evaluate the effect of PA-BSA complexing meth...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10118109/?tool=EBI |
| _version_ | 1797842254652506112 |
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| author | Yanzhuo Yang Qingting Yu Bin Li Zuisu Yang Shuai Zhang Falei Yuan |
| author_facet | Yanzhuo Yang Qingting Yu Bin Li Zuisu Yang Shuai Zhang Falei Yuan |
| author_sort | Yanzhuo Yang |
| collection | DOAJ |
| description | Palmitic acid (PA) is considered a major contributor to the inflammation in many metabolic diseases; however, this role has been questioned recently for the complicated procedures in preparing PA-bovine serum albumin (BSA) complex. This study is aimed to evaluate the effect of PA-BSA complexing methods on cell viability and inflammatory responses of BV-2 cells. Three commercially available BSA brands and two types of solvents were compared for their effects on the expression of inflammatory cytokines. Three commonly used proportions of PA-BSA were tested for cell viability and inflammatory responses. We found that all the three types of BSA were proinflammatory. Both ethanol and isopropanol dampened inflammation except that 1% isopropanol treatment increased the IL-1β level by 26%. When reducing the BSA content in PA-BSA solutions from 3:1 to 5:1, a marked increase in cell viability (11%) was seen. To our surprise, reducing BSA content in PA-BSA solutions from 5:1 to 10:1 decreased cell viability by 11%. The 5:1 group exhibited the lowest inflammatory profile. Either PA-BSA or BSA alone increased the entry of LPS to the cytosol, which further caused pyroptosis. In summary, we found 5:1 (PA:BSA) to be the best binding ratio for studying inflammation in BV-2 microglia. The presence of LPS in the cytosol in the context of BSA might be the reason for confounding results from palmitate studies. |
| first_indexed | 2024-04-09T16:44:58Z |
| format | Article |
| id | doaj.art-9a64a0bb6a694bd9baeb90f3f5accc20 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T16:44:58Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9a64a0bb6a694bd9baeb90f3f5accc202023-04-23T05:31:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01184Palmitate lipotoxicity is closely associated with the fatty acid-albumin complexes in BV-2 microgliaYanzhuo YangQingting YuBin LiZuisu YangShuai ZhangFalei YuanPalmitic acid (PA) is considered a major contributor to the inflammation in many metabolic diseases; however, this role has been questioned recently for the complicated procedures in preparing PA-bovine serum albumin (BSA) complex. This study is aimed to evaluate the effect of PA-BSA complexing methods on cell viability and inflammatory responses of BV-2 cells. Three commercially available BSA brands and two types of solvents were compared for their effects on the expression of inflammatory cytokines. Three commonly used proportions of PA-BSA were tested for cell viability and inflammatory responses. We found that all the three types of BSA were proinflammatory. Both ethanol and isopropanol dampened inflammation except that 1% isopropanol treatment increased the IL-1β level by 26%. When reducing the BSA content in PA-BSA solutions from 3:1 to 5:1, a marked increase in cell viability (11%) was seen. To our surprise, reducing BSA content in PA-BSA solutions from 5:1 to 10:1 decreased cell viability by 11%. The 5:1 group exhibited the lowest inflammatory profile. Either PA-BSA or BSA alone increased the entry of LPS to the cytosol, which further caused pyroptosis. In summary, we found 5:1 (PA:BSA) to be the best binding ratio for studying inflammation in BV-2 microglia. The presence of LPS in the cytosol in the context of BSA might be the reason for confounding results from palmitate studies.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10118109/?tool=EBI |
| spellingShingle | Yanzhuo Yang Qingting Yu Bin Li Zuisu Yang Shuai Zhang Falei Yuan Palmitate lipotoxicity is closely associated with the fatty acid-albumin complexes in BV-2 microglia PLoS ONE |
| title | Palmitate lipotoxicity is closely associated with the fatty acid-albumin complexes in BV-2 microglia |
| title_full | Palmitate lipotoxicity is closely associated with the fatty acid-albumin complexes in BV-2 microglia |
| title_fullStr | Palmitate lipotoxicity is closely associated with the fatty acid-albumin complexes in BV-2 microglia |
| title_full_unstemmed | Palmitate lipotoxicity is closely associated with the fatty acid-albumin complexes in BV-2 microglia |
| title_short | Palmitate lipotoxicity is closely associated with the fatty acid-albumin complexes in BV-2 microglia |
| title_sort | palmitate lipotoxicity is closely associated with the fatty acid albumin complexes in bv 2 microglia |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10118109/?tool=EBI |
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