Self-Diagnosis of SARS-CoV-2 from Saliva Samples at Home: Isothermal Amplification Enabled by Do-It-Yourself Portable Incubators and Laminated Poly-ethyl Sulfonate Membranes
COVID-19 made explicit the need for rethinking the way in which we conduct testing for epidemic emergencies. During the COVID-19 pandemic, the dependence on centralized lab facilities and resource-intensive methodologies (e.g., RT-qPCR methods) greatly limited the deployment of widespread testing ef...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
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| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/14/2/221 |
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| author | Sergio Bravo-González Everardo González-González Valeria Perales-Salinas Iram Pablo Rodríguez-Sánchez Jose E. Ortiz-Castillo Adriana Vargas-Martínez Victor H. Perez-Gonzalez Claudia Maribel Luna-Aguirre Grissel Trujillo-de Santiago Mario Moisés Alvarez |
| author_facet | Sergio Bravo-González Everardo González-González Valeria Perales-Salinas Iram Pablo Rodríguez-Sánchez Jose E. Ortiz-Castillo Adriana Vargas-Martínez Victor H. Perez-Gonzalez Claudia Maribel Luna-Aguirre Grissel Trujillo-de Santiago Mario Moisés Alvarez |
| author_sort | Sergio Bravo-González |
| collection | DOAJ |
| description | COVID-19 made explicit the need for rethinking the way in which we conduct testing for epidemic emergencies. During the COVID-19 pandemic, the dependence on centralized lab facilities and resource-intensive methodologies (e.g., RT-qPCR methods) greatly limited the deployment of widespread testing efforts in many developed and underdeveloped countries. Here, we illustrate the development of a simple and portable diagnostic kit that enables self-diagnosis of COVID-19 at home from saliva samples. We describe the development of a do-it-yourself (DIY) incubator for Eppendorf tubes that can be used to conduct SARS-CoV-2 detection with competitive sensitivity and selectivity from saliva at home. In a proof-of-concept experiment, we assembled Eppendorf-tube incubators at our home shop, prepared a single-tube mix of reagents and LAMP primers in our lab, and deployed these COVID-19 detection kits using urban delivery systems (i.e., Rappifavor or Uber) to more than 15 different locations in Monterrey, México. This straightforward strategy enabled rapid and cost-effective at-home molecular diagnostics of SARS-CoV-2 from real saliva samples with a high sensitivity (100%) and high selectivity (87%). |
| first_indexed | 2024-03-08T11:00:20Z |
| format | Article |
| id | doaj.art-9a64cb788cfe4ce48e30933cd9b557c1 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-08T11:00:20Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-9a64cb788cfe4ce48e30933cd9b557c12024-01-26T16:02:02ZengMDPI AGDiagnostics2075-44182024-01-0114222110.3390/diagnostics14020221Self-Diagnosis of SARS-CoV-2 from Saliva Samples at Home: Isothermal Amplification Enabled by Do-It-Yourself Portable Incubators and Laminated Poly-ethyl Sulfonate MembranesSergio Bravo-González0Everardo González-González1Valeria Perales-Salinas2Iram Pablo Rodríguez-Sánchez3Jose E. Ortiz-Castillo4Adriana Vargas-Martínez5Victor H. Perez-Gonzalez6Claudia Maribel Luna-Aguirre7Grissel Trujillo-de Santiago8Mario Moisés Alvarez9Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, Monterrey 64849, NL, MexicoCentro de Biotecnología-FEMSA, Tecnologico de Monterrey, Monterrey 64849, NL, MexicoCentro de Biotecnología-FEMSA, Tecnologico de Monterrey, Monterrey 64849, NL, MexicoLaboratorio de Fisiología Molecular y Estructural, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, NL, MexicoDepartamento de Ingeniería Mecátrónica y Eléctrica, Tecnologico de Monterrey, Monterrey 64849, NL, MexicoDepartamento de Ingeniería Mecátrónica y Eléctrica, Tecnologico de Monterrey, Monterrey 64849, NL, MexicoDepartamento de Ingeniería Mecátrónica y Eléctrica, Tecnologico de Monterrey, Monterrey 64849, NL, MexicoCentro de Biotecnología-FEMSA, Tecnologico de Monterrey, Monterrey 64849, NL, MexicoCentro de Biotecnología-FEMSA, Tecnologico de Monterrey, Monterrey 64849, NL, MexicoCentro de Biotecnología-FEMSA, Tecnologico de Monterrey, Monterrey 64849, NL, MexicoCOVID-19 made explicit the need for rethinking the way in which we conduct testing for epidemic emergencies. During the COVID-19 pandemic, the dependence on centralized lab facilities and resource-intensive methodologies (e.g., RT-qPCR methods) greatly limited the deployment of widespread testing efforts in many developed and underdeveloped countries. Here, we illustrate the development of a simple and portable diagnostic kit that enables self-diagnosis of COVID-19 at home from saliva samples. We describe the development of a do-it-yourself (DIY) incubator for Eppendorf tubes that can be used to conduct SARS-CoV-2 detection with competitive sensitivity and selectivity from saliva at home. In a proof-of-concept experiment, we assembled Eppendorf-tube incubators at our home shop, prepared a single-tube mix of reagents and LAMP primers in our lab, and deployed these COVID-19 detection kits using urban delivery systems (i.e., Rappifavor or Uber) to more than 15 different locations in Monterrey, México. This straightforward strategy enabled rapid and cost-effective at-home molecular diagnostics of SARS-CoV-2 from real saliva samples with a high sensitivity (100%) and high selectivity (87%).https://www.mdpi.com/2075-4418/14/2/221salivaSARS-CoV-2COVID-19diagnosticportableisothermal nucleic acid amplification |
| spellingShingle | Sergio Bravo-González Everardo González-González Valeria Perales-Salinas Iram Pablo Rodríguez-Sánchez Jose E. Ortiz-Castillo Adriana Vargas-Martínez Victor H. Perez-Gonzalez Claudia Maribel Luna-Aguirre Grissel Trujillo-de Santiago Mario Moisés Alvarez Self-Diagnosis of SARS-CoV-2 from Saliva Samples at Home: Isothermal Amplification Enabled by Do-It-Yourself Portable Incubators and Laminated Poly-ethyl Sulfonate Membranes Diagnostics saliva SARS-CoV-2 COVID-19 diagnostic portable isothermal nucleic acid amplification |
| title | Self-Diagnosis of SARS-CoV-2 from Saliva Samples at Home: Isothermal Amplification Enabled by Do-It-Yourself Portable Incubators and Laminated Poly-ethyl Sulfonate Membranes |
| title_full | Self-Diagnosis of SARS-CoV-2 from Saliva Samples at Home: Isothermal Amplification Enabled by Do-It-Yourself Portable Incubators and Laminated Poly-ethyl Sulfonate Membranes |
| title_fullStr | Self-Diagnosis of SARS-CoV-2 from Saliva Samples at Home: Isothermal Amplification Enabled by Do-It-Yourself Portable Incubators and Laminated Poly-ethyl Sulfonate Membranes |
| title_full_unstemmed | Self-Diagnosis of SARS-CoV-2 from Saliva Samples at Home: Isothermal Amplification Enabled by Do-It-Yourself Portable Incubators and Laminated Poly-ethyl Sulfonate Membranes |
| title_short | Self-Diagnosis of SARS-CoV-2 from Saliva Samples at Home: Isothermal Amplification Enabled by Do-It-Yourself Portable Incubators and Laminated Poly-ethyl Sulfonate Membranes |
| title_sort | self diagnosis of sars cov 2 from saliva samples at home isothermal amplification enabled by do it yourself portable incubators and laminated poly ethyl sulfonate membranes |
| topic | saliva SARS-CoV-2 COVID-19 diagnostic portable isothermal nucleic acid amplification |
| url | https://www.mdpi.com/2075-4418/14/2/221 |
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