Identification and characterisation of oil sludge degrading bacteria isolated from compost
Compounds present in oil sludge such as polycyclic aromatic hydrocarbons (PAHs) are known to be cytotoxic, mutagenic and potentially carcinogenic. Microorganisms including bacteria and fungi have been reported to degrade oil sludge components to innocuous compounds such as carbon dioxide, water and...
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2016-06-01
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| Series: | Archives of Environmental Protection |
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| Online Access: | http://www.degruyter.com/view/j/aep.2016.42.issue-2/aep-2016-0021/aep-2016-0021.xml?format=INT |
| _version_ | 1797423926752575488 |
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| author | Ubani Onyedikachi Atagana Harrison Ifeanyichukwu Thantsha Mapitsi Silvester Rasheed Adeleke |
| author_facet | Ubani Onyedikachi Atagana Harrison Ifeanyichukwu Thantsha Mapitsi Silvester Rasheed Adeleke |
| author_sort | Ubani Onyedikachi |
| collection | DOAJ |
| description | Compounds present in oil sludge such as polycyclic aromatic hydrocarbons (PAHs) are known to be cytotoxic, mutagenic and potentially carcinogenic. Microorganisms including bacteria and fungi have been reported to degrade oil sludge components to innocuous compounds such as carbon dioxide, water and salts. In the present study, we isolated different bacteria with PAH-degrading capabilities from compost prepared from oil sludge and animal manures. These bacteria were isolated on a mineral base medium and mineral salt agar plates. A total of 31 morphologically distinct isolates were carefully selected from 5 different compost treatments for identification using polymerase chain reaction (PCR) of the 16S rRNA gene with specific primers (universal forward 16S-P1 PCR and reverse 16S-P2 PCR). The amplicons were sequenced and sequences were compared with the known nucleotides from the GenBank. The phylogenetic analyses of the isolates showed that they belong to 3 different clades; Firmicutes, Proteobacteria and Actinobacteria. These bacteria identified were closely related to the genera Bacillus, Arthrobacter, Staphylococcus, Brevibacterium, Variovorax, Paenibacillus, Ralstonia and Geobacillus. The results showed that Bacillus species were predominant in all composts. Based on the results of the degradation of the PAHs in the composts and results of previous studies on bacterial degradation of hydrocarbons in oil, the characteristics of these bacterial isolates suggests that they may be responsible for the breakdown of PAHs of different molecular weights in the composts. Thus, they may be potentially useful for bioremediation of oil sludge during compost bioremediation. |
| first_indexed | 2024-03-09T07:54:46Z |
| format | Article |
| id | doaj.art-9a670d884aa04df4a7f9b19663a0dc99 |
| institution | Directory Open Access Journal |
| issn | 2083-4810 |
| language | English |
| last_indexed | 2024-03-09T07:54:46Z |
| publishDate | 2016-06-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Archives of Environmental Protection |
| spelling | doaj.art-9a670d884aa04df4a7f9b19663a0dc992023-12-03T01:17:25ZengPolish Academy of SciencesArchives of Environmental Protection2083-48102016-06-01422677710.1515/aep-2016-0021aep-2016-0021Identification and characterisation of oil sludge degrading bacteria isolated from compostUbani Onyedikachi0Atagana Harrison Ifeanyichukwu1Thantsha Mapitsi Silvester2Rasheed Adeleke3Department of Environmental Sciences, University of South Africa, South AfricaInstitute for Science and Technology Education, University of South Africa, South AfricaDepartment of Microbiology and Plant Pathology, University of Pretoria, South AfricaClimate and Water, Agricultural Research Council, South Africa Institute for SoilCompounds present in oil sludge such as polycyclic aromatic hydrocarbons (PAHs) are known to be cytotoxic, mutagenic and potentially carcinogenic. Microorganisms including bacteria and fungi have been reported to degrade oil sludge components to innocuous compounds such as carbon dioxide, water and salts. In the present study, we isolated different bacteria with PAH-degrading capabilities from compost prepared from oil sludge and animal manures. These bacteria were isolated on a mineral base medium and mineral salt agar plates. A total of 31 morphologically distinct isolates were carefully selected from 5 different compost treatments for identification using polymerase chain reaction (PCR) of the 16S rRNA gene with specific primers (universal forward 16S-P1 PCR and reverse 16S-P2 PCR). The amplicons were sequenced and sequences were compared with the known nucleotides from the GenBank. The phylogenetic analyses of the isolates showed that they belong to 3 different clades; Firmicutes, Proteobacteria and Actinobacteria. These bacteria identified were closely related to the genera Bacillus, Arthrobacter, Staphylococcus, Brevibacterium, Variovorax, Paenibacillus, Ralstonia and Geobacillus. The results showed that Bacillus species were predominant in all composts. Based on the results of the degradation of the PAHs in the composts and results of previous studies on bacterial degradation of hydrocarbons in oil, the characteristics of these bacterial isolates suggests that they may be responsible for the breakdown of PAHs of different molecular weights in the composts. Thus, they may be potentially useful for bioremediation of oil sludge during compost bioremediation.http://www.degruyter.com/view/j/aep.2016.42.issue-2/aep-2016-0021/aep-2016-0021.xml?format=INTBioaugmentationbiodegradationbioremediationcompostingoil sludgePAHsanimal manures |
| spellingShingle | Ubani Onyedikachi Atagana Harrison Ifeanyichukwu Thantsha Mapitsi Silvester Rasheed Adeleke Identification and characterisation of oil sludge degrading bacteria isolated from compost Archives of Environmental Protection Bioaugmentation biodegradation bioremediation composting oil sludge PAHs animal manures |
| title | Identification and characterisation of oil sludge degrading bacteria isolated from compost |
| title_full | Identification and characterisation of oil sludge degrading bacteria isolated from compost |
| title_fullStr | Identification and characterisation of oil sludge degrading bacteria isolated from compost |
| title_full_unstemmed | Identification and characterisation of oil sludge degrading bacteria isolated from compost |
| title_short | Identification and characterisation of oil sludge degrading bacteria isolated from compost |
| title_sort | identification and characterisation of oil sludge degrading bacteria isolated from compost |
| topic | Bioaugmentation biodegradation bioremediation composting oil sludge PAHs animal manures |
| url | http://www.degruyter.com/view/j/aep.2016.42.issue-2/aep-2016-0021/aep-2016-0021.xml?format=INT |
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