Local YB-1, Epo, and EpoR concentrations in fractured bones: results from a porcine model of multiple trauma
Abstract Little is known about the impact of multiple trauma (MT)-related systemic hypoxia on osseous protein concentration of the hypoxia transcriptome. To shed light on this issue, we investigated erythropoietin (Epo), erythropoietin receptor (EpoR), and Y-box binding protein 1 (YB-1) concentratio...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-023-00996-w |
| _version_ | 1797952637583228928 |
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| author | Felix Marius Bläsius Johannes Greven Weijun Guo Eftychios Bolierakis Zhizhen He Cavan Lübke Tim-Philipp Simon Frank Hildebrand Klemens Horst |
| author_facet | Felix Marius Bläsius Johannes Greven Weijun Guo Eftychios Bolierakis Zhizhen He Cavan Lübke Tim-Philipp Simon Frank Hildebrand Klemens Horst |
| author_sort | Felix Marius Bläsius |
| collection | DOAJ |
| description | Abstract Little is known about the impact of multiple trauma (MT)-related systemic hypoxia on osseous protein concentration of the hypoxia transcriptome. To shed light on this issue, we investigated erythropoietin (Epo), erythropoietin receptor (EpoR), and Y-box binding protein 1 (YB-1) concentrations in the fracture zone in a porcine MT + traumatic hemorrhage (TH) model. Sixteen male domestic pigs were randomized into two groups: an MT + TH group and a sham group. A tibia fracture, lung contusion, and TH were induced in the MT + TH group. The total observation period was 72 h. YB-1 concentrations in bone marrow (BM) were significantly lower in the fracture zone of the MT + TH animals than in the sham animals. Significant downregulation of BM-localized EpoR concentration in both unfractured and fractured bones was observed in the MT + TH animals relative to the sham animals. In BM, Epo concentrations were higher in the fracture zone of the MT + TH animals compared with that in the sham animals. Significantly higher Epo concentrations were detected in the BM of fractured bone compared to that in cortical bone. Our results provide the first evidence that MT + TH alters hypoxia-related protein concentrations. The impacts of both the fracture and concomitant injuries on protein concentrations need to be studied in more detail to shed light on the hypoxia transcriptome in fractured and healthy bones after MT + TH. |
| first_indexed | 2024-04-10T22:49:34Z |
| format | Article |
| id | doaj.art-9a6925dde0a0475495cf59f2fc09ddf5 |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-04-10T22:49:34Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-9a6925dde0a0475495cf59f2fc09ddf52023-01-15T12:07:35ZengBMCEuropean Journal of Medical Research2047-783X2023-01-0128111010.1186/s40001-023-00996-wLocal YB-1, Epo, and EpoR concentrations in fractured bones: results from a porcine model of multiple traumaFelix Marius Bläsius0Johannes Greven1Weijun Guo2Eftychios Bolierakis3Zhizhen He4Cavan Lübke5Tim-Philipp Simon6Frank Hildebrand7Klemens Horst8Deptartment of Orthopaedics, Trauma and Reconstructive Surgery, University Hospital, RWTH UniversityDeptartment of Orthopaedics, Trauma and Reconstructive Surgery, University Hospital, RWTH UniversityDeptartment of Orthopaedics, Trauma and Reconstructive Surgery, University Hospital, RWTH UniversityDeptartment of Orthopaedics, Trauma and Reconstructive Surgery, University Hospital, RWTH UniversityDeptartment of Orthopaedics, Trauma and Reconstructive Surgery, University Hospital, RWTH UniversityDepartment of Intensive Care and Intermediate Care, University Hospital, RWTH UniversityDepartment of Intensive Care and Intermediate Care, University Hospital, RWTH UniversityDeptartment of Orthopaedics, Trauma and Reconstructive Surgery, University Hospital, RWTH UniversityDeptartment of Orthopaedics, Trauma and Reconstructive Surgery, University Hospital, RWTH UniversityAbstract Little is known about the impact of multiple trauma (MT)-related systemic hypoxia on osseous protein concentration of the hypoxia transcriptome. To shed light on this issue, we investigated erythropoietin (Epo), erythropoietin receptor (EpoR), and Y-box binding protein 1 (YB-1) concentrations in the fracture zone in a porcine MT + traumatic hemorrhage (TH) model. Sixteen male domestic pigs were randomized into two groups: an MT + TH group and a sham group. A tibia fracture, lung contusion, and TH were induced in the MT + TH group. The total observation period was 72 h. YB-1 concentrations in bone marrow (BM) were significantly lower in the fracture zone of the MT + TH animals than in the sham animals. Significant downregulation of BM-localized EpoR concentration in both unfractured and fractured bones was observed in the MT + TH animals relative to the sham animals. In BM, Epo concentrations were higher in the fracture zone of the MT + TH animals compared with that in the sham animals. Significantly higher Epo concentrations were detected in the BM of fractured bone compared to that in cortical bone. Our results provide the first evidence that MT + TH alters hypoxia-related protein concentrations. The impacts of both the fracture and concomitant injuries on protein concentrations need to be studied in more detail to shed light on the hypoxia transcriptome in fractured and healthy bones after MT + TH.https://doi.org/10.1186/s40001-023-00996-wYB-1ErythropoietinEpoErythropoietin receptorMultiple traumaTraumatic hemorrhage |
| spellingShingle | Felix Marius Bläsius Johannes Greven Weijun Guo Eftychios Bolierakis Zhizhen He Cavan Lübke Tim-Philipp Simon Frank Hildebrand Klemens Horst Local YB-1, Epo, and EpoR concentrations in fractured bones: results from a porcine model of multiple trauma European Journal of Medical Research YB-1 Erythropoietin Epo Erythropoietin receptor Multiple trauma Traumatic hemorrhage |
| title | Local YB-1, Epo, and EpoR concentrations in fractured bones: results from a porcine model of multiple trauma |
| title_full | Local YB-1, Epo, and EpoR concentrations in fractured bones: results from a porcine model of multiple trauma |
| title_fullStr | Local YB-1, Epo, and EpoR concentrations in fractured bones: results from a porcine model of multiple trauma |
| title_full_unstemmed | Local YB-1, Epo, and EpoR concentrations in fractured bones: results from a porcine model of multiple trauma |
| title_short | Local YB-1, Epo, and EpoR concentrations in fractured bones: results from a porcine model of multiple trauma |
| title_sort | local yb 1 epo and epor concentrations in fractured bones results from a porcine model of multiple trauma |
| topic | YB-1 Erythropoietin Epo Erythropoietin receptor Multiple trauma Traumatic hemorrhage |
| url | https://doi.org/10.1186/s40001-023-00996-w |
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