Effect of diluent variation on cryopreservation of large yellow croaker Larimichthys crocea
The aim of this research was to investigate different factors, including cryoprotective agents (CPAs), diluents, dilution ratios, equilibrium times, freezing rates, and thawing methods to optimize cryopreservation protocols for large yellow...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Korean Society of Fisheries and Aquatic Science
2021-02-01
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| Series: | Fisheries and Aquatic Sciences |
| Subjects: | |
| Online Access: | http://www.e-fas.org/archive/view_article?pid=fas-24-2-63 |
| _version_ | 1818156302773256192 |
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| author | Han Kyu Lim Zidni Irfan Hyo Bin Lee Ji Hoon Song Yun Ho Lee |
| author_facet | Han Kyu Lim Zidni Irfan Hyo Bin Lee Ji Hoon Song Yun Ho Lee |
| author_sort | Han Kyu Lim |
| collection | DOAJ |
| description | The aim of this research was to investigate different factors, including
cryoprotective agents (CPAs), diluents, dilution ratios, equilibrium times,
freezing rates, and thawing methods to optimize cryopreservation protocols for
large yellow croaker (Larimichthys crocea). The parameters
evaluated were sperm motility, sperm activity index (SAI), survival rate, and
DNA damage. Different types of CPAs, such as dimethyl sulfoxide (DMSO),
propylene glycol (PG), ethylene glycol (EG), methanol, and glycerol, were tested
for sperm preservation. The highest motility, SAI, and survival rate were
observed when EG was used. Different diluents such as Stein’s solution,
Hank’s balanced salt solution, marine fish Ringer’s solution,
artificial seminal plasma (ASP) of small yellow croaker, and Cortland solution
were investigated. The highest post-thaw motility was observed upon using ASP as
the diluent. Different concentrations of EG were then mixed with ASP to identify
the optimal EG concentration. Experimental results showed that the motility
(70.33 ± 1.20%), SAI (5), and survival rate (78.30 ± 0.42%) of
post-thaw sperm were optimum when 10% EG and ASP were used as the CPA and
diluent of cryopreservation, respectively. Post-thaw sperm motility was high at
equilibration times below 150 s and at an optimum dilution ratio of 1:1 (sperm:
CPA + diluent) and was not significantly different compared with fresh sperm
motility. The freezing rate was found to be slow below −10°C/min.
The thawing temperature of 45°C was identified as ideal. The percentage
of tail DNA in post-thaw sperm at 10% EG and ASP was also investigated and was
found to have more significant DNA damage than that in fresh sperm but
significantly lower damage than that in post-thaw sperm at EG concentrations of
5%, 15%, and 20% (p < 0.05). The cryopreservation
protocols obtained in this study will be useful in large yellow croaker
hatcheries. |
| first_indexed | 2024-12-11T14:56:09Z |
| format | Article |
| id | doaj.art-9a69765824d943cc9887bd447a28d8f2 |
| institution | Directory Open Access Journal |
| issn | 2234-1757 |
| language | English |
| last_indexed | 2024-12-11T14:56:09Z |
| publishDate | 2021-02-01 |
| publisher | The Korean Society of Fisheries and Aquatic Science |
| record_format | Article |
| series | Fisheries and Aquatic Sciences |
| spelling | doaj.art-9a69765824d943cc9887bd447a28d8f22022-12-22T01:01:19ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572021-02-01242637710.47853/FAS.2021.e7fas-24-2-63Effect of diluent variation on cryopreservation of large yellow croaker Larimichthys croceaHan Kyu Lim0Zidni Irfan1Hyo Bin Lee2Ji Hoon Song3Yun Ho Lee4Department of Interdisciplinary Program of Biomedicine, Health & Life Convergence Sciences, Mokpo National University, Mokpo 58554, KoreaDepartment of Interdisciplinary Program of Biomedicine, Health & Life Convergence Sciences, Mokpo National University, Mokpo 58554, KoreaDepartment of Interdisciplinary Program of Biomedicine, Health & Life Convergence Sciences, Mokpo National University, Mokpo 58554, KoreaOcean & Fisheries Science Institute Resources Creation Research Institute, Shinan-gun 58813, KoreaDepartment of Interdisciplinary Program of Biomedicine, Health & Life Convergence Sciences, Mokpo National University, Mokpo 58554, KoreaThe aim of this research was to investigate different factors, including cryoprotective agents (CPAs), diluents, dilution ratios, equilibrium times, freezing rates, and thawing methods to optimize cryopreservation protocols for large yellow croaker (Larimichthys crocea). The parameters evaluated were sperm motility, sperm activity index (SAI), survival rate, and DNA damage. Different types of CPAs, such as dimethyl sulfoxide (DMSO), propylene glycol (PG), ethylene glycol (EG), methanol, and glycerol, were tested for sperm preservation. The highest motility, SAI, and survival rate were observed when EG was used. Different diluents such as Stein’s solution, Hank’s balanced salt solution, marine fish Ringer’s solution, artificial seminal plasma (ASP) of small yellow croaker, and Cortland solution were investigated. The highest post-thaw motility was observed upon using ASP as the diluent. Different concentrations of EG were then mixed with ASP to identify the optimal EG concentration. Experimental results showed that the motility (70.33 ± 1.20%), SAI (5), and survival rate (78.30 ± 0.42%) of post-thaw sperm were optimum when 10% EG and ASP were used as the CPA and diluent of cryopreservation, respectively. Post-thaw sperm motility was high at equilibration times below 150 s and at an optimum dilution ratio of 1:1 (sperm: CPA + diluent) and was not significantly different compared with fresh sperm motility. The freezing rate was found to be slow below −10°C/min. The thawing temperature of 45°C was identified as ideal. The percentage of tail DNA in post-thaw sperm at 10% EG and ASP was also investigated and was found to have more significant DNA damage than that in fresh sperm but significantly lower damage than that in post-thaw sperm at EG concentrations of 5%, 15%, and 20% (p < 0.05). The cryopreservation protocols obtained in this study will be useful in large yellow croaker hatcheries.http://www.e-fas.org/archive/view_article?pid=fas-24-2-63cryopreservationcryoprotective agentssperm motilitylarge yellow croaker |
| spellingShingle | Han Kyu Lim Zidni Irfan Hyo Bin Lee Ji Hoon Song Yun Ho Lee Effect of diluent variation on cryopreservation of large yellow croaker Larimichthys crocea Fisheries and Aquatic Sciences cryopreservation cryoprotective agents sperm motility large yellow croaker |
| title | Effect of diluent variation on cryopreservation of large yellow
croaker Larimichthys crocea |
| title_full | Effect of diluent variation on cryopreservation of large yellow
croaker Larimichthys crocea |
| title_fullStr | Effect of diluent variation on cryopreservation of large yellow
croaker Larimichthys crocea |
| title_full_unstemmed | Effect of diluent variation on cryopreservation of large yellow
croaker Larimichthys crocea |
| title_short | Effect of diluent variation on cryopreservation of large yellow
croaker Larimichthys crocea |
| title_sort | effect of diluent variation on cryopreservation of large yellow croaker larimichthys crocea |
| topic | cryopreservation cryoprotective agents sperm motility large yellow croaker |
| url | http://www.e-fas.org/archive/view_article?pid=fas-24-2-63 |
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