Generation of induced pluripotent stem cell line (RCMGi009-A) from urine cells of patient with fibrodysplasia ossificans progressiva
Urine cells obtained from a 14-year-old man with genetically proven (ACVR1: c.6176G > A) and clinically manifested fibrodysplasia ossificans progressiva were successfully transformed into induced pluripotent stem cells by using Sendai virus-based reprogramming vectors including the four Yamanaka...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506123001198 |
| _version_ | 1827923912233582592 |
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| author | Ekaterina Kondrateva Olga Grigorieva Elizaveta Kurshakova Irina Panchuk Victoria Pozhitnova Ekaterina Voronina Vyacheslav Tabakov Maria Orlova Alexander Lavrov Svetlana Smirnikhina Sergey Kutsev |
| author_facet | Ekaterina Kondrateva Olga Grigorieva Elizaveta Kurshakova Irina Panchuk Victoria Pozhitnova Ekaterina Voronina Vyacheslav Tabakov Maria Orlova Alexander Lavrov Svetlana Smirnikhina Sergey Kutsev |
| author_sort | Ekaterina Kondrateva |
| collection | DOAJ |
| description | Urine cells obtained from a 14-year-old man with genetically proven (ACVR1: c.6176G > A) and clinically manifested fibrodysplasia ossificans progressiva were successfully transformed into induced pluripotent stem cells by using Sendai virus-based reprogramming vectors including the four Yamanaka factors such as OCT3/4, SOX2, KLF4, and c-MYC. These iPSCs expressed pluripotency markers, exhibited the potential to differentiate into three germ layers in spontaneous differentiation assay and had a normal karyotype. The iPSC line may provide a model for development of a personalized treatment including genome editing and drug screening, may be used for disease modelling, cell differentiation and pharmacological investigations.. |
| first_indexed | 2024-03-13T05:03:08Z |
| format | Article |
| id | doaj.art-9a6e25fd826248969e58a1e427c2ad38 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-13T05:03:08Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-9a6e25fd826248969e58a1e427c2ad382023-06-17T05:17:54ZengElsevierStem Cell Research1873-50612023-08-0170103133Generation of induced pluripotent stem cell line (RCMGi009-A) from urine cells of patient with fibrodysplasia ossificans progressivaEkaterina Kondrateva0Olga Grigorieva1Elizaveta Kurshakova2Irina Panchuk3Victoria Pozhitnova4Ekaterina Voronina5Vyacheslav Tabakov6Maria Orlova7Alexander Lavrov8Svetlana Smirnikhina9Sergey Kutsev10Corresponding author.; Research Centre for Medical Genetics, Moscow 115522, Russian FederationResearch Centre for Medical Genetics, Moscow 115522, Russian FederationResearch Centre for Medical Genetics, Moscow 115522, Russian FederationResearch Centre for Medical Genetics, Moscow 115522, Russian FederationResearch Centre for Medical Genetics, Moscow 115522, Russian FederationResearch Centre for Medical Genetics, Moscow 115522, Russian FederationResearch Centre for Medical Genetics, Moscow 115522, Russian FederationResearch Centre for Medical Genetics, Moscow 115522, Russian FederationResearch Centre for Medical Genetics, Moscow 115522, Russian FederationResearch Centre for Medical Genetics, Moscow 115522, Russian FederationResearch Centre for Medical Genetics, Moscow 115522, Russian FederationUrine cells obtained from a 14-year-old man with genetically proven (ACVR1: c.6176G > A) and clinically manifested fibrodysplasia ossificans progressiva were successfully transformed into induced pluripotent stem cells by using Sendai virus-based reprogramming vectors including the four Yamanaka factors such as OCT3/4, SOX2, KLF4, and c-MYC. These iPSCs expressed pluripotency markers, exhibited the potential to differentiate into three germ layers in spontaneous differentiation assay and had a normal karyotype. The iPSC line may provide a model for development of a personalized treatment including genome editing and drug screening, may be used for disease modelling, cell differentiation and pharmacological investigations..http://www.sciencedirect.com/science/article/pii/S1873506123001198 |
| spellingShingle | Ekaterina Kondrateva Olga Grigorieva Elizaveta Kurshakova Irina Panchuk Victoria Pozhitnova Ekaterina Voronina Vyacheslav Tabakov Maria Orlova Alexander Lavrov Svetlana Smirnikhina Sergey Kutsev Generation of induced pluripotent stem cell line (RCMGi009-A) from urine cells of patient with fibrodysplasia ossificans progressiva Stem Cell Research |
| title | Generation of induced pluripotent stem cell line (RCMGi009-A) from urine cells of patient with fibrodysplasia ossificans progressiva |
| title_full | Generation of induced pluripotent stem cell line (RCMGi009-A) from urine cells of patient with fibrodysplasia ossificans progressiva |
| title_fullStr | Generation of induced pluripotent stem cell line (RCMGi009-A) from urine cells of patient with fibrodysplasia ossificans progressiva |
| title_full_unstemmed | Generation of induced pluripotent stem cell line (RCMGi009-A) from urine cells of patient with fibrodysplasia ossificans progressiva |
| title_short | Generation of induced pluripotent stem cell line (RCMGi009-A) from urine cells of patient with fibrodysplasia ossificans progressiva |
| title_sort | generation of induced pluripotent stem cell line rcmgi009 a from urine cells of patient with fibrodysplasia ossificans progressiva |
| url | http://www.sciencedirect.com/science/article/pii/S1873506123001198 |
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