Molecular Identification of Six Honeybee Viruses in Iranian Apiaries
The identification of honeybee viruses is of serious importance, particularly considering the lack of information on the natural incidence of viral infections in honeybee populations worldwide. Moreover, the global spread of Varroa destructor in honeybee colonies has a significant effect on the vira...
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| Format: | Article |
| Language: | English |
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Razi Vaccine and Serum Research Institute
2018-12-01
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| Series: | Archives of Razi Institute |
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| Online Access: | http://archrazi.areeo.ac.ir/article_117278_f3d50235510f0883071eba140e948d9e.pdf |
| _version_ | 1811277759545081856 |
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| author | M. Moharrami H. Modirrousta |
| author_facet | M. Moharrami H. Modirrousta |
| author_sort | M. Moharrami |
| collection | DOAJ |
| description | The identification of honeybee viruses is of serious importance, particularly considering the lack of information on the natural incidence of viral infections in honeybee populations worldwide. Moreover, the global spread of Varroa destructor in honeybee colonies has a significant effect on the viral infection. In the present study, 160 samples of adult bee from apparently healthy colonies but with a background of parasitic diseases, tremor, and paralysis, were collected during 2011-2012. The samples belonged to 23 different provinces of Iran. They were sent to Razi Vaccine and Serum Research Institute, Karaj, Iran, for further analysis, and examined for the presence of viruses using reverse transcription polymerase chain reaction assay. According to the results, out of 160 samples, 9 (5.8 %), 40 (25.6 %), 12 (7.8 %), 34 (21.8 %), 7 (4.5 %), and 29 (18.5%) cases were positive for acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), Kashmir bee virus (KBV), and sacbrood virus (SBV). The samples collected from 18 provinces (78 %) were positive for at least one virus. Among all samples, 83 (53.2 %) specimens were infected with at least one virus. The highest prevalent virus was BQCV, followed by DWV, SBV, CBPV, ABPV, and KBV, respectively. |
| first_indexed | 2024-04-13T00:22:38Z |
| format | Article |
| id | doaj.art-9a6ff5300f0548b197dea199f160c8d9 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-04-13T00:22:38Z |
| publishDate | 2018-12-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-9a6ff5300f0548b197dea199f160c8d92022-12-22T03:10:43ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722018-12-0173431131810.22092/ari.2017.107481.1068117278Molecular Identification of Six Honeybee Viruses in Iranian ApiariesM. Moharrami0H. Modirrousta1Department of Honeybee, silk worm and wildlife disease, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranDepartment of Honeybee, silk worm and wildlife disease, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranThe identification of honeybee viruses is of serious importance, particularly considering the lack of information on the natural incidence of viral infections in honeybee populations worldwide. Moreover, the global spread of Varroa destructor in honeybee colonies has a significant effect on the viral infection. In the present study, 160 samples of adult bee from apparently healthy colonies but with a background of parasitic diseases, tremor, and paralysis, were collected during 2011-2012. The samples belonged to 23 different provinces of Iran. They were sent to Razi Vaccine and Serum Research Institute, Karaj, Iran, for further analysis, and examined for the presence of viruses using reverse transcription polymerase chain reaction assay. According to the results, out of 160 samples, 9 (5.8 %), 40 (25.6 %), 12 (7.8 %), 34 (21.8 %), 7 (4.5 %), and 29 (18.5%) cases were positive for acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), Kashmir bee virus (KBV), and sacbrood virus (SBV). The samples collected from 18 provinces (78 %) were positive for at least one virus. Among all samples, 83 (53.2 %) specimens were infected with at least one virus. The highest prevalent virus was BQCV, followed by DWV, SBV, CBPV, ABPV, and KBV, respectively.http://archrazi.areeo.ac.ir/article_117278_f3d50235510f0883071eba140e948d9e.pdfHoney bee viruses (ABPV-BQCV-CBPV- DWV- KBV-SBV)RT-PCRIran |
| spellingShingle | M. Moharrami H. Modirrousta Molecular Identification of Six Honeybee Viruses in Iranian Apiaries Archives of Razi Institute Honey bee viruses (ABPV-BQCV-CBPV- DWV- KBV-SBV) RT-PCR Iran |
| title | Molecular Identification of Six Honeybee Viruses in Iranian Apiaries |
| title_full | Molecular Identification of Six Honeybee Viruses in Iranian Apiaries |
| title_fullStr | Molecular Identification of Six Honeybee Viruses in Iranian Apiaries |
| title_full_unstemmed | Molecular Identification of Six Honeybee Viruses in Iranian Apiaries |
| title_short | Molecular Identification of Six Honeybee Viruses in Iranian Apiaries |
| title_sort | molecular identification of six honeybee viruses in iranian apiaries |
| topic | Honey bee viruses (ABPV-BQCV-CBPV- DWV- KBV-SBV) RT-PCR Iran |
| url | http://archrazi.areeo.ac.ir/article_117278_f3d50235510f0883071eba140e948d9e.pdf |
| work_keys_str_mv | AT mmoharrami molecularidentificationofsixhoneybeevirusesiniranianapiaries AT hmodirrousta molecularidentificationofsixhoneybeevirusesiniranianapiaries |