Industrial Production of Proteins with <i>Pichia pastoris</i>—<i>Komagataella phaffii</i>
Since the mid-1960s, methylotrophic yeast <i>Komagataella phaffii</i> (previously described as <i>Pichia pastoris</i>) has received increasing scientific attention. The interest for the industrial production of proteins for different applications (e.g., feed, food additives,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/3/441 |
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| author | Giovanni Davide Barone Anita Emmerstorfer-Augustin Antonino Biundo Isabella Pisano Paola Coccetti Valeria Mapelli Andrea Camattari |
| author_facet | Giovanni Davide Barone Anita Emmerstorfer-Augustin Antonino Biundo Isabella Pisano Paola Coccetti Valeria Mapelli Andrea Camattari |
| author_sort | Giovanni Davide Barone |
| collection | DOAJ |
| description | Since the mid-1960s, methylotrophic yeast <i>Komagataella phaffii</i> (previously described as <i>Pichia pastoris</i>) has received increasing scientific attention. The interest for the industrial production of proteins for different applications (e.g., feed, food additives, detergent, waste treatment processes, and textile) is a well-consolidated scientific topic, and the importance for this approach is rising in the current era of environmental transition in human societies. This review aims to summarize fundamental and specific information in this scientific field. Additionally, an updated description of the relevant products produced with <i>K</i>. <i>phaffii</i> at industrial levels by a variety of companies—describing how the industry has leveraged its key features, from products for the ingredients of meat-free burgers (e.g., IMPOSSIBLE™ FOODS, USA) to diabetes therapeutics (e.g., Biocon, India)—is provided. Furthermore, active patents and the typical workflow for industrial protein production with this strain are reported. |
| first_indexed | 2024-03-11T06:52:45Z |
| format | Article |
| id | doaj.art-9a70349496834edc895e582103978099 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T06:52:45Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-9a70349496834edc895e5821039780992023-11-17T09:51:20ZengMDPI AGBiomolecules2218-273X2023-02-0113344110.3390/biom13030441Industrial Production of Proteins with <i>Pichia pastoris</i>—<i>Komagataella phaffii</i>Giovanni Davide Barone0Anita Emmerstorfer-Augustin1Antonino Biundo2Isabella Pisano3Paola Coccetti4Valeria Mapelli5Andrea Camattari6Institute of Molecular Biotechnology, Graz University of Technology, NAWI Graz, 8010 Graz, AustriaInstitute of Molecular Biotechnology, Graz University of Technology, NAWI Graz, 8010 Graz, AustriaDepartment of Biosciences, Biotechnology and Biopharmaceutics, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, ItalyDepartment of Biosciences, Biotechnology and Biopharmaceutics, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, ItalyDepartment of Biotechnology and Biosciences, University of Milano-Bicocca, 20126 Milano, ItalyDepartment of Biotechnology and Biosciences, University of Milano-Bicocca, 20126 Milano, ItalyGINKGO BIOWORKS, 27 Drydock Avenue, 8th Floor Boston, Boston, MA 02210, USASince the mid-1960s, methylotrophic yeast <i>Komagataella phaffii</i> (previously described as <i>Pichia pastoris</i>) has received increasing scientific attention. The interest for the industrial production of proteins for different applications (e.g., feed, food additives, detergent, waste treatment processes, and textile) is a well-consolidated scientific topic, and the importance for this approach is rising in the current era of environmental transition in human societies. This review aims to summarize fundamental and specific information in this scientific field. Additionally, an updated description of the relevant products produced with <i>K</i>. <i>phaffii</i> at industrial levels by a variety of companies—describing how the industry has leveraged its key features, from products for the ingredients of meat-free burgers (e.g., IMPOSSIBLE™ FOODS, USA) to diabetes therapeutics (e.g., Biocon, India)—is provided. Furthermore, active patents and the typical workflow for industrial protein production with this strain are reported.https://www.mdpi.com/2218-273X/13/3/441<i>Pichia pastoris</i><i>Komagataella phaffii</i>methylotrophic yeastprotein productionbiotechnologyapplied biotechnology |
| spellingShingle | Giovanni Davide Barone Anita Emmerstorfer-Augustin Antonino Biundo Isabella Pisano Paola Coccetti Valeria Mapelli Andrea Camattari Industrial Production of Proteins with <i>Pichia pastoris</i>—<i>Komagataella phaffii</i> Biomolecules <i>Pichia pastoris</i> <i>Komagataella phaffii</i> methylotrophic yeast protein production biotechnology applied biotechnology |
| title | Industrial Production of Proteins with <i>Pichia pastoris</i>—<i>Komagataella phaffii</i> |
| title_full | Industrial Production of Proteins with <i>Pichia pastoris</i>—<i>Komagataella phaffii</i> |
| title_fullStr | Industrial Production of Proteins with <i>Pichia pastoris</i>—<i>Komagataella phaffii</i> |
| title_full_unstemmed | Industrial Production of Proteins with <i>Pichia pastoris</i>—<i>Komagataella phaffii</i> |
| title_short | Industrial Production of Proteins with <i>Pichia pastoris</i>—<i>Komagataella phaffii</i> |
| title_sort | industrial production of proteins with i pichia pastoris i i komagataella phaffii i |
| topic | <i>Pichia pastoris</i> <i>Komagataella phaffii</i> methylotrophic yeast protein production biotechnology applied biotechnology |
| url | https://www.mdpi.com/2218-273X/13/3/441 |
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