Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of <em>Gambierdiscus polynesiensis</em>
<i>Gambierdiscus</i> and <i>Fukuyoa</i> dinoflagellates produce a suite of secondary metabolites, including ciguatoxins (CTXs), which bioaccumulate and are further biotransformed in fish and marine invertebrates, causing ciguatera poisoning when consumed by humans. This study...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/20/6/348 |
| _version_ | 1797485025557479424 |
|---|---|
| author | Hélène Taiana Darius Taina Revel Jérôme Viallon Manoëlla Sibat Philippe Cruchet Sébastien Longo Donnie Ransom Hardison William C. Holland Patricia A. Tester R. Wayne Litaker Jennifer R. McCall Philipp Hess Mireille Chinain |
| author_facet | Hélène Taiana Darius Taina Revel Jérôme Viallon Manoëlla Sibat Philippe Cruchet Sébastien Longo Donnie Ransom Hardison William C. Holland Patricia A. Tester R. Wayne Litaker Jennifer R. McCall Philipp Hess Mireille Chinain |
| author_sort | Hélène Taiana Darius |
| collection | DOAJ |
| description | <i>Gambierdiscus</i> and <i>Fukuyoa</i> dinoflagellates produce a suite of secondary metabolites, including ciguatoxins (CTXs), which bioaccumulate and are further biotransformed in fish and marine invertebrates, causing ciguatera poisoning when consumed by humans. This study is the first to compare the performance of the fluorescent receptor binding assay (fRBA), neuroblastoma cell-based assay (CBA-N2a), and liquid chromatography tandem mass spectrometry (LC-MS/MS) for the quantitative estimation of CTX contents in 30 samples, obtained from four French Polynesian strains of <i>Gambierdiscus polynesiensis</i>. fRBA was applied to <i>Gambierdiscus</i> matrix for the first time, and several parameters of the fRBA protocol were refined. Following liquid/liquid partitioning to separate CTXs from other algal compounds, the variability of CTX contents was estimated using these three methods in three independent experiments. All three assays were significantly correlated with each other, with the highest correlation coefficient (<i>r</i><sup>2</sup> = 0.841) found between fRBA and LC-MS/MS. The CBA-N2a was more sensitive than LC-MS/MS and fRBA, with all assays showing good repeatability. The combined use of fRBA and/or CBA-N2a for screening purposes and LC-MS/MS for confirmation purposes allows for efficient CTX evaluation in <i>Gambierdiscus</i>. These findings, which support future collaborative studies for the inter-laboratory validation of CTX detection methods, will help improve ciguatera risk assessment and management. |
| first_indexed | 2024-03-09T23:13:51Z |
| format | Article |
| id | doaj.art-9a7209740bb54777bbc8bf6cd0cfe61d |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T23:13:51Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-9a7209740bb54777bbc8bf6cd0cfe61d2023-11-23T17:40:41ZengMDPI AGMarine Drugs1660-33972022-05-0120634810.3390/md20060348Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of <em>Gambierdiscus polynesiensis</em>Hélène Taiana Darius0Taina Revel1Jérôme Viallon2Manoëlla Sibat3Philippe Cruchet4Sébastien Longo5Donnie Ransom Hardison6William C. Holland7Patricia A. Tester8R. Wayne Litaker9Jennifer R. McCall10Philipp Hess11Mireille Chinain12Institut Louis Malardé (ILM), Laboratory of Marine Biotoxins, UMR 241-EIO (IFREMER, ILM, IRD, Université de Polynésie Française), P.O. Box 30, Papeete 98713, French PolynesiaInstitut Louis Malardé (ILM), Laboratory of Marine Biotoxins, UMR 241-EIO (IFREMER, ILM, IRD, Université de Polynésie Française), P.O. Box 30, Papeete 98713, French PolynesiaInstitut Louis Malardé (ILM), Laboratory of Marine Biotoxins, UMR 241-EIO (IFREMER, ILM, IRD, Université de Polynésie Française), P.O. Box 30, Papeete 98713, French PolynesiaIFREMER, PHYTOX, Laboratoire METALG, F-44000 Nantes, FranceInstitut Louis Malardé (ILM), Laboratory of Marine Biotoxins, UMR 241-EIO (IFREMER, ILM, IRD, Université de Polynésie Française), P.O. Box 30, Papeete 98713, French PolynesiaInstitut Louis Malardé (ILM), Laboratory of Marine Biotoxins, UMR 241-EIO (IFREMER, ILM, IRD, Université de Polynésie Française), P.O. Box 30, Papeete 98713, French PolynesiaNational Oceanic and Atmospheric Administration, Center for Coastal Fisheries and Habitat Research, Beaufort, NC 28516, USANational Oceanic and Atmospheric Administration, Center for Coastal Fisheries and Habitat Research, Beaufort, NC 28516, USAOcean Tester, LLC, Beaufort, NC 28516, USACSS, Inc. Under Contract to National Oceanic and Atmospheric Administration, National Centers for Coastal Ocean Science, National Ocean Service, Beaufort, NC 28516, USACenter for Marine Science, University of North Carolina Wilmington, 601 South College Road, Wilmington, NC 28403, USAIFREMER, PHYTOX, Laboratoire METALG, F-44000 Nantes, FranceInstitut Louis Malardé (ILM), Laboratory of Marine Biotoxins, UMR 241-EIO (IFREMER, ILM, IRD, Université de Polynésie Française), P.O. Box 30, Papeete 98713, French Polynesia<i>Gambierdiscus</i> and <i>Fukuyoa</i> dinoflagellates produce a suite of secondary metabolites, including ciguatoxins (CTXs), which bioaccumulate and are further biotransformed in fish and marine invertebrates, causing ciguatera poisoning when consumed by humans. This study is the first to compare the performance of the fluorescent receptor binding assay (fRBA), neuroblastoma cell-based assay (CBA-N2a), and liquid chromatography tandem mass spectrometry (LC-MS/MS) for the quantitative estimation of CTX contents in 30 samples, obtained from four French Polynesian strains of <i>Gambierdiscus polynesiensis</i>. fRBA was applied to <i>Gambierdiscus</i> matrix for the first time, and several parameters of the fRBA protocol were refined. Following liquid/liquid partitioning to separate CTXs from other algal compounds, the variability of CTX contents was estimated using these three methods in three independent experiments. All three assays were significantly correlated with each other, with the highest correlation coefficient (<i>r</i><sup>2</sup> = 0.841) found between fRBA and LC-MS/MS. The CBA-N2a was more sensitive than LC-MS/MS and fRBA, with all assays showing good repeatability. The combined use of fRBA and/or CBA-N2a for screening purposes and LC-MS/MS for confirmation purposes allows for efficient CTX evaluation in <i>Gambierdiscus</i>. These findings, which support future collaborative studies for the inter-laboratory validation of CTX detection methods, will help improve ciguatera risk assessment and management.https://www.mdpi.com/1660-3397/20/6/348ciguatera poisoning<i>Gambierdiscus polynesiensis</i>ciguatoxinsfluorescent receptor binding assayneuroblastoma cell-based assayliquid chromatography tandem mass spectrometry |
| spellingShingle | Hélène Taiana Darius Taina Revel Jérôme Viallon Manoëlla Sibat Philippe Cruchet Sébastien Longo Donnie Ransom Hardison William C. Holland Patricia A. Tester R. Wayne Litaker Jennifer R. McCall Philipp Hess Mireille Chinain Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of <em>Gambierdiscus polynesiensis</em> Marine Drugs ciguatera poisoning <i>Gambierdiscus polynesiensis</i> ciguatoxins fluorescent receptor binding assay neuroblastoma cell-based assay liquid chromatography tandem mass spectrometry |
| title | Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of <em>Gambierdiscus polynesiensis</em> |
| title_full | Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of <em>Gambierdiscus polynesiensis</em> |
| title_fullStr | Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of <em>Gambierdiscus polynesiensis</em> |
| title_full_unstemmed | Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of <em>Gambierdiscus polynesiensis</em> |
| title_short | Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of <em>Gambierdiscus polynesiensis</em> |
| title_sort | comparative study on the performance of three detection methods for the quantification of pacific ciguatoxins in french polynesian strains of em gambierdiscus polynesiensis em |
| topic | ciguatera poisoning <i>Gambierdiscus polynesiensis</i> ciguatoxins fluorescent receptor binding assay neuroblastoma cell-based assay liquid chromatography tandem mass spectrometry |
| url | https://www.mdpi.com/1660-3397/20/6/348 |
| work_keys_str_mv | AT helenetaianadarius comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem AT tainarevel comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem AT jeromeviallon comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem AT manoellasibat comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem AT philippecruchet comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem AT sebastienlongo comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem AT donnieransomhardison comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem AT williamcholland comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem AT patriciaatester comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem AT rwaynelitaker comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem AT jenniferrmccall comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem AT philipphess comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem AT mireillechinain comparativestudyontheperformanceofthreedetectionmethodsforthequantificationofpacificciguatoxinsinfrenchpolynesianstrainsofemgambierdiscuspolynesiensisem |