The Effect of Alpha-Lipoic Acid against Methotrexate on Testicular Damage in Rats
Aim: The toxic effects of methotrexate, a chemotherapeutic, on the testicles is an important side effect. Methotrexate impairs spermatogenesis and fertility and causes oligospermia. In this study, we aimed to minimize the testicular toxicity, those being the side effects of methotrexate, by using th...
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| Format: | Article |
| Language: | English |
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Rabia Yılmaz
2023-03-01
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| Series: | Journal of Contemporary Medicine |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/2919519 |
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| author | Ahmetcan Varel Meltem Özgöçmen Hamit Hakan Armağan İbrahim Aydın Candan Dilek Bayram İlkay Armağan |
| author_facet | Ahmetcan Varel Meltem Özgöçmen Hamit Hakan Armağan İbrahim Aydın Candan Dilek Bayram İlkay Armağan |
| author_sort | Ahmetcan Varel |
| collection | DOAJ |
| description | Aim: The toxic effects of methotrexate, a chemotherapeutic, on the testicles is an important side effect. Methotrexate impairs spermatogenesis and fertility and causes oligospermia. In this study, we aimed to minimize the testicular toxicity, those being the side effects of methotrexate, by using the probable protective effects of α-lipoic acid, a potent antioxidant.
Materials and Methods: Twenty-eight male Sprague Dawley rats that we employed in this research were separated into three groups as control (0.09% PS) (n=8), methotrexate (20 mg/kg) (n= 10), and methotrexate (20 mg/kg) + α-lipoic acid (100 mg/kg) (n= 10). We performed a histochemical analysis on the testicular tissue of rats using hematoxylin-eosin and Masson’s trichrome. We performed an immunohistochemical analysis using inducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-α) primer ab.
Results: The histochemical evaluation revealed a significant decrease in the methotrexate-induced testicular toxicity in the α-lipoic acid-treated groups. On the other hand, TNF-α and iNOS immunostaining results were also observed to support these results.
Conclusion: The treatment use of α-lipoic acid succeeded in protecting against methotrexate-induced testicular damage through an α-lipoic acid-mediated antioxidant and anti-inflammatory mechanisms. α-lipoic acid can be used in combination with methotrexate as a protector against side effects during anticancer therapy. In the present study, it was shown that α-lipoic acid can be used in combination with methotrexate as a protector against side effects during anticancer treatment. |
| first_indexed | 2024-04-09T21:50:55Z |
| format | Article |
| id | doaj.art-9a7253cf0d314910bd44fd2f1fb8d8bd |
| institution | Directory Open Access Journal |
| issn | 2667-7180 |
| language | English |
| last_indexed | 2024-04-09T21:50:55Z |
| publishDate | 2023-03-01 |
| publisher | Rabia Yılmaz |
| record_format | Article |
| series | Journal of Contemporary Medicine |
| spelling | doaj.art-9a7253cf0d314910bd44fd2f1fb8d8bd2023-03-24T19:43:20ZengRabia YılmazJournal of Contemporary Medicine2667-71802023-03-0113233534110.16899/jcm.12429031809The Effect of Alpha-Lipoic Acid against Methotrexate on Testicular Damage in RatsAhmetcan Varel0Meltem Özgöçmen1Hamit Hakan Armağan2İbrahim Aydın Candan3Dilek Bayram4İlkay Armağan5SULEYMAN DEMIREL UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGYSULEYMAN DEMIREL UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGYSULEYMAN DEMIREL UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF INTERNAL MEDICINE, DEPARTMENT OF ACADEMIC EMERGENCY MEDICINEALANYA ALAADDIN KEYKUBAT UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES (MEDICINE), DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY (MEDICINE)SULEYMAN DEMIREL UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGYSULEYMAN DEMIREL UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGYAim: The toxic effects of methotrexate, a chemotherapeutic, on the testicles is an important side effect. Methotrexate impairs spermatogenesis and fertility and causes oligospermia. In this study, we aimed to minimize the testicular toxicity, those being the side effects of methotrexate, by using the probable protective effects of α-lipoic acid, a potent antioxidant. Materials and Methods: Twenty-eight male Sprague Dawley rats that we employed in this research were separated into three groups as control (0.09% PS) (n=8), methotrexate (20 mg/kg) (n= 10), and methotrexate (20 mg/kg) + α-lipoic acid (100 mg/kg) (n= 10). We performed a histochemical analysis on the testicular tissue of rats using hematoxylin-eosin and Masson’s trichrome. We performed an immunohistochemical analysis using inducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-α) primer ab. Results: The histochemical evaluation revealed a significant decrease in the methotrexate-induced testicular toxicity in the α-lipoic acid-treated groups. On the other hand, TNF-α and iNOS immunostaining results were also observed to support these results. Conclusion: The treatment use of α-lipoic acid succeeded in protecting against methotrexate-induced testicular damage through an α-lipoic acid-mediated antioxidant and anti-inflammatory mechanisms. α-lipoic acid can be used in combination with methotrexate as a protector against side effects during anticancer therapy. In the present study, it was shown that α-lipoic acid can be used in combination with methotrexate as a protector against side effects during anticancer treatment.https://dergipark.org.tr/tr/download/article-file/2919519alpha-lipoic acidanti-inflammatorymethotrexatetesticular damageoxidative stressalfa-lipoik asitantiinflamatuvarmetotreksattestis hasarıoksidatif stres |
| spellingShingle | Ahmetcan Varel Meltem Özgöçmen Hamit Hakan Armağan İbrahim Aydın Candan Dilek Bayram İlkay Armağan The Effect of Alpha-Lipoic Acid against Methotrexate on Testicular Damage in Rats Journal of Contemporary Medicine alpha-lipoic acid anti-inflammatory methotrexate testicular damage oxidative stress alfa-lipoik asit antiinflamatuvar metotreksat testis hasarı oksidatif stres |
| title | The Effect of Alpha-Lipoic Acid against Methotrexate on Testicular Damage in Rats |
| title_full | The Effect of Alpha-Lipoic Acid against Methotrexate on Testicular Damage in Rats |
| title_fullStr | The Effect of Alpha-Lipoic Acid against Methotrexate on Testicular Damage in Rats |
| title_full_unstemmed | The Effect of Alpha-Lipoic Acid against Methotrexate on Testicular Damage in Rats |
| title_short | The Effect of Alpha-Lipoic Acid against Methotrexate on Testicular Damage in Rats |
| title_sort | effect of alpha lipoic acid against methotrexate on testicular damage in rats |
| topic | alpha-lipoic acid anti-inflammatory methotrexate testicular damage oxidative stress alfa-lipoik asit antiinflamatuvar metotreksat testis hasarı oksidatif stres |
| url | https://dergipark.org.tr/tr/download/article-file/2919519 |
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