GIFT: An ImageJ macro for automated fiber diameter quantification.
This paper details the development and testing of the GIFT macro, which is a freely available program for ImageJ for the automated measurement of fiber diameters in SEM images of electrospun materials. The GIFT macro applies a validated method which distinguishes fiber diameters based on distance fr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0275528 |
| _version_ | 1811341447190806528 |
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| author | Jennifer Huling Andreas Götz Niels Grabow Sabine Illner |
| author_facet | Jennifer Huling Andreas Götz Niels Grabow Sabine Illner |
| author_sort | Jennifer Huling |
| collection | DOAJ |
| description | This paper details the development and testing of the GIFT macro, which is a freely available program for ImageJ for the automated measurement of fiber diameters in SEM images of electrospun materials. The GIFT macro applies a validated method which distinguishes fiber diameters based on distance frequencies within an image. In this work, we introduce an applied version of the GIFT method which has been designed to be user-friendly while still allowing complete control over the various parameters involved in the image processing steps. The macro quickly processes large data sets and creates results that are reproducible and accurate. The program outputs both raw data and fiber diameter averages, so that the user can quickly assess the results and has the opportunity for further analysis if desired. The GIFT macro was compared directly to other software designed for fiber diameter measurements and was found to have comparable or lower average error, especially when measuring very small fibers, and reduced processing times per image. The macro, detailed instructions for use, and sample images are freely available online (https://github.com/IBMTRostock/GIFT). We believe that the GIFT macro is a valuable new tool for researchers looking to quickly, easily and reliably assess fiber diameters in electrospun materials. |
| first_indexed | 2024-04-13T18:56:33Z |
| format | Article |
| id | doaj.art-9a7348c7eb684c4ea17f4e935fed1ce6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T18:56:33Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9a7348c7eb684c4ea17f4e935fed1ce62022-12-22T02:34:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011710e027552810.1371/journal.pone.0275528GIFT: An ImageJ macro for automated fiber diameter quantification.Jennifer HulingAndreas GötzNiels GrabowSabine IllnerThis paper details the development and testing of the GIFT macro, which is a freely available program for ImageJ for the automated measurement of fiber diameters in SEM images of electrospun materials. The GIFT macro applies a validated method which distinguishes fiber diameters based on distance frequencies within an image. In this work, we introduce an applied version of the GIFT method which has been designed to be user-friendly while still allowing complete control over the various parameters involved in the image processing steps. The macro quickly processes large data sets and creates results that are reproducible and accurate. The program outputs both raw data and fiber diameter averages, so that the user can quickly assess the results and has the opportunity for further analysis if desired. The GIFT macro was compared directly to other software designed for fiber diameter measurements and was found to have comparable or lower average error, especially when measuring very small fibers, and reduced processing times per image. The macro, detailed instructions for use, and sample images are freely available online (https://github.com/IBMTRostock/GIFT). We believe that the GIFT macro is a valuable new tool for researchers looking to quickly, easily and reliably assess fiber diameters in electrospun materials.https://doi.org/10.1371/journal.pone.0275528 |
| spellingShingle | Jennifer Huling Andreas Götz Niels Grabow Sabine Illner GIFT: An ImageJ macro for automated fiber diameter quantification. PLoS ONE |
| title | GIFT: An ImageJ macro for automated fiber diameter quantification. |
| title_full | GIFT: An ImageJ macro for automated fiber diameter quantification. |
| title_fullStr | GIFT: An ImageJ macro for automated fiber diameter quantification. |
| title_full_unstemmed | GIFT: An ImageJ macro for automated fiber diameter quantification. |
| title_short | GIFT: An ImageJ macro for automated fiber diameter quantification. |
| title_sort | gift an imagej macro for automated fiber diameter quantification |
| url | https://doi.org/10.1371/journal.pone.0275528 |
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