The Co-Culture of Staphylococcal Biofilm and Fibroblast Cell Line: The Correlation of Biological Phenomena with Metabolic NMR<sup>1</sup> Footprint
<i>Staphylococcus aureus</i> is one of the most prevalent pathogens associated with several types of biofilm-based infections, including infections of chronic wounds. Mature staphylococcal biofilm is extremely hard to eradicate from a wound and displays a high tendency to induce recurrin...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/11/5826 |
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| author | Joanna Czajkowska Adam Junka Jakub Hoppe Monika Toporkiewicz Andrzej Pawlak Paweł Migdał Monika Oleksy-Wawrzyniak Karol Fijałkowski Marcin Śmiglak Agata Markowska-Szczupak |
| author_facet | Joanna Czajkowska Adam Junka Jakub Hoppe Monika Toporkiewicz Andrzej Pawlak Paweł Migdał Monika Oleksy-Wawrzyniak Karol Fijałkowski Marcin Śmiglak Agata Markowska-Szczupak |
| author_sort | Joanna Czajkowska |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> is one of the most prevalent pathogens associated with several types of biofilm-based infections, including infections of chronic wounds. Mature staphylococcal biofilm is extremely hard to eradicate from a wound and displays a high tendency to induce recurring infections. Therefore, in the present study, we aimed to investigate in vitro the interaction between <i>S. aureus</i> biofilm and fibroblast cells searching for metabolites that could be considered as potential biomarkers of critical colonization and infection. Utilizing advanced microscopy and microbiological methods to examine biofilm formation and the staphylococcal infection process, we were able to distinguish 4 phases of biofilm development. The analysis of staphylococcal biofilm influence on the viability of fibroblasts allowed us to pinpoint the moment of critical colonization—12 h post contamination. Based on the obtained model we performed a metabolomics analysis by <sup>1</sup>H NMR spectroscopy to provide new insights into the pathophysiology of infection. We identified a set of metabolites related to the switch to anaerobic metabolism that was characteristic for staphylococcal biofilm co-cultured with fibroblast cells. The data presented in this study may be thus considered a noteworthy but preliminary step in the direction of developing a new, NMR-based tool for rapid diagnosing of infection in a chronic wound. |
| first_indexed | 2024-03-10T10:54:47Z |
| format | Article |
| id | doaj.art-9a7484f42b294b13b818ca7f1a2582f9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:54:47Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9a7484f42b294b13b818ca7f1a2582f92023-11-21T21:59:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-012211582610.3390/ijms22115826The Co-Culture of Staphylococcal Biofilm and Fibroblast Cell Line: The Correlation of Biological Phenomena with Metabolic NMR<sup>1</sup> FootprintJoanna Czajkowska0Adam Junka1Jakub Hoppe2Monika Toporkiewicz3Andrzej Pawlak4Paweł Migdał5Monika Oleksy-Wawrzyniak6Karol Fijałkowski7Marcin Śmiglak8Agata Markowska-Szczupak9Laboratory of Microbiology, Łukasiewicz Research Network–PORT Polish Center for Technology Development, 54-066 Wrocław, PolandLaboratory of Microbiology, Łukasiewicz Research Network–PORT Polish Center for Technology Development, 54-066 Wrocław, PolandPoznan Science and Technology Park (PPNT), Rubiez 5, 61-612 Poznań, PolandBioimaging Laboratory, Łukasiewicz Research Network—PORT Polish Center for Technology Development, 54-066 Wrocław, PolandDepartment of Nervous System Diseases, Kazimierza Bartla 5, 50-996 Wrocław, PolandDepartment of Environment Hygiene and Animal Welfare, Wroclaw University of Environmental and Life Sciences, 51-630 Wroclaw, PolandDepartment of Chemical and Process Engineering, West Pomeranian University of Technology, 71-065 Szczecin, PolandDepartment of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, 70-311 Szczecin, PolandPoznan Science and Technology Park (PPNT), Rubiez 5, 61-612 Poznań, PolandDepartment of Chemical and Process Engineering, West Pomeranian University of Technology, 71-065 Szczecin, Poland<i>Staphylococcus aureus</i> is one of the most prevalent pathogens associated with several types of biofilm-based infections, including infections of chronic wounds. Mature staphylococcal biofilm is extremely hard to eradicate from a wound and displays a high tendency to induce recurring infections. Therefore, in the present study, we aimed to investigate in vitro the interaction between <i>S. aureus</i> biofilm and fibroblast cells searching for metabolites that could be considered as potential biomarkers of critical colonization and infection. Utilizing advanced microscopy and microbiological methods to examine biofilm formation and the staphylococcal infection process, we were able to distinguish 4 phases of biofilm development. The analysis of staphylococcal biofilm influence on the viability of fibroblasts allowed us to pinpoint the moment of critical colonization—12 h post contamination. Based on the obtained model we performed a metabolomics analysis by <sup>1</sup>H NMR spectroscopy to provide new insights into the pathophysiology of infection. We identified a set of metabolites related to the switch to anaerobic metabolism that was characteristic for staphylococcal biofilm co-cultured with fibroblast cells. The data presented in this study may be thus considered a noteworthy but preliminary step in the direction of developing a new, NMR-based tool for rapid diagnosing of infection in a chronic wound.https://www.mdpi.com/1422-0067/22/11/5826<i>S. aureus</i>fibroblastsco-cultureinfectionmetabolic profilesNMR |
| spellingShingle | Joanna Czajkowska Adam Junka Jakub Hoppe Monika Toporkiewicz Andrzej Pawlak Paweł Migdał Monika Oleksy-Wawrzyniak Karol Fijałkowski Marcin Śmiglak Agata Markowska-Szczupak The Co-Culture of Staphylococcal Biofilm and Fibroblast Cell Line: The Correlation of Biological Phenomena with Metabolic NMR<sup>1</sup> Footprint International Journal of Molecular Sciences <i>S. aureus</i> fibroblasts co-culture infection metabolic profiles NMR |
| title | The Co-Culture of Staphylococcal Biofilm and Fibroblast Cell Line: The Correlation of Biological Phenomena with Metabolic NMR<sup>1</sup> Footprint |
| title_full | The Co-Culture of Staphylococcal Biofilm and Fibroblast Cell Line: The Correlation of Biological Phenomena with Metabolic NMR<sup>1</sup> Footprint |
| title_fullStr | The Co-Culture of Staphylococcal Biofilm and Fibroblast Cell Line: The Correlation of Biological Phenomena with Metabolic NMR<sup>1</sup> Footprint |
| title_full_unstemmed | The Co-Culture of Staphylococcal Biofilm and Fibroblast Cell Line: The Correlation of Biological Phenomena with Metabolic NMR<sup>1</sup> Footprint |
| title_short | The Co-Culture of Staphylococcal Biofilm and Fibroblast Cell Line: The Correlation of Biological Phenomena with Metabolic NMR<sup>1</sup> Footprint |
| title_sort | co culture of staphylococcal biofilm and fibroblast cell line the correlation of biological phenomena with metabolic nmr sup 1 sup footprint |
| topic | <i>S. aureus</i> fibroblasts co-culture infection metabolic profiles NMR |
| url | https://www.mdpi.com/1422-0067/22/11/5826 |
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