Water Extract of Portulaca Oleracea Inhibits PEDV Infection-Induced Pyrolysis by Caspase-1/GSDMD
Porcine epidemic diarrhea virus (PEDV) belongs to the coronavirus family and the coronavirus genus, causing contact enteric infection in pigs. It is one of the most serious diseases that threatens the pig industry. However, there is currently no specific drug to prevent and treat the disease, indica...
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MDPI AG
2023-12-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/12/637 |
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| author | Yu Zhang Yueyue Liu Shifa Yang Bin Yin Zengcheng Zhao Zhongli Huang Jiaqiang Wu Shuqian Lin Xin Wang |
| author_facet | Yu Zhang Yueyue Liu Shifa Yang Bin Yin Zengcheng Zhao Zhongli Huang Jiaqiang Wu Shuqian Lin Xin Wang |
| author_sort | Yu Zhang |
| collection | DOAJ |
| description | Porcine epidemic diarrhea virus (PEDV) belongs to the coronavirus family and the coronavirus genus, causing contact enteric infection in pigs. It is one of the most serious diseases that threatens the pig industry. However, there is currently no specific drug to prevent and treat the disease, indicating that we need to be vigilant about the spread of the disease and the development of anti-PEDV drugs. The dried aerial parts of the plant <i>Portulaca oleracea</i> in the family Portulacaceous, whose decoction can be used to treat acute enteritis, dysentery, diarrhea, and other diseases. This study explored the potential mechanism of water extract of <i>Portulaca oleracea</i> (WEPO) in PEDV-induced pyroptosis in Vero cells. PEDV decreased the viability of Vero cells in a dose- and time-dependent manner, causing cell damage, upregulating the level of intracellular Nlrp3, and inhibiting the level of Gasdermin D (GSDMD) and the activation of Caspase-1. WEPO can inhibit PEDV-induced pyroptosis, reduce the elevation of inflammatory factors caused by infection, and exhibit a dose-dependent effect. Knockdown of Caspase-1 and GSDMD separately can induce the production of the inflammatory factor IL-1β to significantly decrease and increase, respectively. These results suggest that WEPO can inhibit cell pyroptosis caused by PEDV and that the Caspase-1 and GSDMD pathways play an important role in this process. |
| first_indexed | 2024-03-08T20:53:53Z |
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| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-08T20:53:53Z |
| publishDate | 2023-12-01 |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-9a77ff18c74648699a2c18737dda2d1f2023-12-22T14:00:46ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-12-014512102111022410.3390/cimb45120637Water Extract of Portulaca Oleracea Inhibits PEDV Infection-Induced Pyrolysis by Caspase-1/GSDMDYu Zhang0Yueyue Liu1Shifa Yang2Bin Yin3Zengcheng Zhao4Zhongli Huang5Jiaqiang Wu6Shuqian Lin7Xin Wang8College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, ChinaInstitute of Poultry Science, Shandong Academy of Agricultural Science, Shandong Provincial Animal and Poultry Green Health Products Creation Engineering Laboratory, Jinan 250100, ChinaInstitute of Poultry Science, Shandong Academy of Agricultural Science, Shandong Provincial Animal and Poultry Green Health Products Creation Engineering Laboratory, Jinan 250100, ChinaInstitute of Poultry Science, Shandong Academy of Agricultural Science, Shandong Provincial Animal and Poultry Green Health Products Creation Engineering Laboratory, Jinan 250100, ChinaInstitute of Poultry Science, Shandong Academy of Agricultural Science, Shandong Provincial Animal and Poultry Green Health Products Creation Engineering Laboratory, Jinan 250100, ChinaInstitute of Poultry Science, Shandong Academy of Agricultural Science, Shandong Provincial Animal and Poultry Green Health Products Creation Engineering Laboratory, Jinan 250100, ChinaInstitute of Poultry Science, Shandong Academy of Agricultural Science, Shandong Provincial Animal and Poultry Green Health Products Creation Engineering Laboratory, Jinan 250100, ChinaInstitute of Poultry Science, Shandong Academy of Agricultural Science, Shandong Provincial Animal and Poultry Green Health Products Creation Engineering Laboratory, Jinan 250100, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, ChinaPorcine epidemic diarrhea virus (PEDV) belongs to the coronavirus family and the coronavirus genus, causing contact enteric infection in pigs. It is one of the most serious diseases that threatens the pig industry. However, there is currently no specific drug to prevent and treat the disease, indicating that we need to be vigilant about the spread of the disease and the development of anti-PEDV drugs. The dried aerial parts of the plant <i>Portulaca oleracea</i> in the family Portulacaceous, whose decoction can be used to treat acute enteritis, dysentery, diarrhea, and other diseases. This study explored the potential mechanism of water extract of <i>Portulaca oleracea</i> (WEPO) in PEDV-induced pyroptosis in Vero cells. PEDV decreased the viability of Vero cells in a dose- and time-dependent manner, causing cell damage, upregulating the level of intracellular Nlrp3, and inhibiting the level of Gasdermin D (GSDMD) and the activation of Caspase-1. WEPO can inhibit PEDV-induced pyroptosis, reduce the elevation of inflammatory factors caused by infection, and exhibit a dose-dependent effect. Knockdown of Caspase-1 and GSDMD separately can induce the production of the inflammatory factor IL-1β to significantly decrease and increase, respectively. These results suggest that WEPO can inhibit cell pyroptosis caused by PEDV and that the Caspase-1 and GSDMD pathways play an important role in this process.https://www.mdpi.com/1467-3045/45/12/637PEDVpyrolysiscaspase-1/GSDMD<i>Portulaca oleracea</i>in-vitro analysis |
| spellingShingle | Yu Zhang Yueyue Liu Shifa Yang Bin Yin Zengcheng Zhao Zhongli Huang Jiaqiang Wu Shuqian Lin Xin Wang Water Extract of Portulaca Oleracea Inhibits PEDV Infection-Induced Pyrolysis by Caspase-1/GSDMD Current Issues in Molecular Biology PEDV pyrolysis caspase-1/GSDMD <i>Portulaca oleracea</i> in-vitro analysis |
| title | Water Extract of Portulaca Oleracea Inhibits PEDV Infection-Induced Pyrolysis by Caspase-1/GSDMD |
| title_full | Water Extract of Portulaca Oleracea Inhibits PEDV Infection-Induced Pyrolysis by Caspase-1/GSDMD |
| title_fullStr | Water Extract of Portulaca Oleracea Inhibits PEDV Infection-Induced Pyrolysis by Caspase-1/GSDMD |
| title_full_unstemmed | Water Extract of Portulaca Oleracea Inhibits PEDV Infection-Induced Pyrolysis by Caspase-1/GSDMD |
| title_short | Water Extract of Portulaca Oleracea Inhibits PEDV Infection-Induced Pyrolysis by Caspase-1/GSDMD |
| title_sort | water extract of portulaca oleracea inhibits pedv infection induced pyrolysis by caspase 1 gsdmd |
| topic | PEDV pyrolysis caspase-1/GSDMD <i>Portulaca oleracea</i> in-vitro analysis |
| url | https://www.mdpi.com/1467-3045/45/12/637 |
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