KERAGAMAN GENETIK VARIETAS PADI <em>japonica</em> DAN <em>indica</em> BERDASARKAN MARKA DNA TERKAIT MUTU RASA

PCR-based markers and evaluation of physicochemical properties should be addressed for the improvement of rice varieties with good eating dan eating quality (EQ). This study aimed to examine the genetic diversity of rice varieties based on DNA markers related to physicochemical properties determinin...

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Main Authors: Puji Lestari, Reflinur Reflinur, Dody Dwi Handoko, Mastur Mastur
Format: Article
Language:Indonesian
Published: Jenderal Soedirman University 2018-03-01
Series:Scripta Biologica
Subjects:
Online Access:https://journal.bio.unsoed.ac.id/index.php/scribio/article/view/751
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author Puji Lestari
Reflinur Reflinur
Dody Dwi Handoko
Mastur Mastur
author_facet Puji Lestari
Reflinur Reflinur
Dody Dwi Handoko
Mastur Mastur
author_sort Puji Lestari
collection DOAJ
description PCR-based markers and evaluation of physicochemical properties should be addressed for the improvement of rice varieties with good eating dan eating quality (EQ). This study aimed to examine the genetic diversity of rice varieties based on DNA markers related to physicochemical properties determining EQ. A total of 46 rice varieties consisting of 22 japonica varieties and 24 indica varieties were examined using 43 PCR-based markers. The results showed that polymorphic information content (PIC) ranged from 0.04 to 0.38, in support of genetic diversity indices which ranged from 0.04 to 0.50 across total markers. Pairwise genetic similarity matrix ranged from 0.40 to 0.98 with the closest genetic distance was observed between two japonica varieties (Dongjin and Hwaseong) and the most distant one was between japonica and indica (Onnuri/Manmi with Cigeulis/Fatmawati). The unweighted neighbor-joining tree clustered the rice varieties into two major clades, indica and japonica, and subsequent subclades were differentiating according to the individual genetic background. The genetic diversity of rice from different subspecies and DNA markers for EQ can effectively be utilized for basic information and marker-assisted selection (MAS) for the development of improved varieties with good EQ in rice breeding program.
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spelling doaj.art-9a78611d4733480b9503dc3cfca3e05d2022-12-21T19:00:06ZindJenderal Soedirman UniversityScripta Biologica2355-31382018-03-015110.20884/1.sb.2018.5.1.751409KERAGAMAN GENETIK VARIETAS PADI <em>japonica</em> DAN <em>indica</em> BERDASARKAN MARKA DNA TERKAIT MUTU RASAPuji Lestari0Reflinur Reflinur1Dody Dwi Handoko2Mastur Mastur3Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian-BalitbangtanBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian-BalitbangtanBalai Besar Penelitian Tanaman Padi-BalitbangtanBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian-BalitbangtanPCR-based markers and evaluation of physicochemical properties should be addressed for the improvement of rice varieties with good eating dan eating quality (EQ). This study aimed to examine the genetic diversity of rice varieties based on DNA markers related to physicochemical properties determining EQ. A total of 46 rice varieties consisting of 22 japonica varieties and 24 indica varieties were examined using 43 PCR-based markers. The results showed that polymorphic information content (PIC) ranged from 0.04 to 0.38, in support of genetic diversity indices which ranged from 0.04 to 0.50 across total markers. Pairwise genetic similarity matrix ranged from 0.40 to 0.98 with the closest genetic distance was observed between two japonica varieties (Dongjin and Hwaseong) and the most distant one was between japonica and indica (Onnuri/Manmi with Cigeulis/Fatmawati). The unweighted neighbor-joining tree clustered the rice varieties into two major clades, indica and japonica, and subsequent subclades were differentiating according to the individual genetic background. The genetic diversity of rice from different subspecies and DNA markers for EQ can effectively be utilized for basic information and marker-assisted selection (MAS) for the development of improved varieties with good EQ in rice breeding program.https://journal.bio.unsoed.ac.id/index.php/scribio/article/view/751eating qualitygenetic diversityricejaponicaindicaDNA marker
spellingShingle Puji Lestari
Reflinur Reflinur
Dody Dwi Handoko
Mastur Mastur
KERAGAMAN GENETIK VARIETAS PADI <em>japonica</em> DAN <em>indica</em> BERDASARKAN MARKA DNA TERKAIT MUTU RASA
Scripta Biologica
eating quality
genetic diversity
rice
japonica
indica
DNA marker
title KERAGAMAN GENETIK VARIETAS PADI <em>japonica</em> DAN <em>indica</em> BERDASARKAN MARKA DNA TERKAIT MUTU RASA
title_full KERAGAMAN GENETIK VARIETAS PADI <em>japonica</em> DAN <em>indica</em> BERDASARKAN MARKA DNA TERKAIT MUTU RASA
title_fullStr KERAGAMAN GENETIK VARIETAS PADI <em>japonica</em> DAN <em>indica</em> BERDASARKAN MARKA DNA TERKAIT MUTU RASA
title_full_unstemmed KERAGAMAN GENETIK VARIETAS PADI <em>japonica</em> DAN <em>indica</em> BERDASARKAN MARKA DNA TERKAIT MUTU RASA
title_short KERAGAMAN GENETIK VARIETAS PADI <em>japonica</em> DAN <em>indica</em> BERDASARKAN MARKA DNA TERKAIT MUTU RASA
title_sort keragaman genetik varietas padi em japonica em dan em indica em berdasarkan marka dna terkait mutu rasa
topic eating quality
genetic diversity
rice
japonica
indica
DNA marker
url https://journal.bio.unsoed.ac.id/index.php/scribio/article/view/751
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AT dodydwihandoko keragamangenetikvarietaspadiemjaponicaemdanemindicaemberdasarkanmarkadnaterkaitmuturasa
AT masturmastur keragamangenetikvarietaspadiemjaponicaemdanemindicaemberdasarkanmarkadnaterkaitmuturasa