Long-Term Tracking of the Effects of Colostrum-Derived <i>Lacticaseibacillus rhamnosus</i> Probio-M9 on Gut Microbiota in Mice with Colitis-Associated Tumorigenesis
Intestinal bacteria play important roles in the progression of colitis-associated carcinogenesis. Colostrum-derived <i>Lacticaseibacillus rhamnosus</i> Probio-M9 (Probio-M9) has shown a protective effect in a colitis-associated cancer (CAC) model, but detailed metagenomic analysis had no...
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MDPI AG
2024-02-01
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| author | Feiyan Zhao Keizo Hiraishi Xiaodong Li Yaopeng Hu Daibo Kojima Zhihong Sun Heping Zhang Lin-Hai Kurahara |
| author_facet | Feiyan Zhao Keizo Hiraishi Xiaodong Li Yaopeng Hu Daibo Kojima Zhihong Sun Heping Zhang Lin-Hai Kurahara |
| author_sort | Feiyan Zhao |
| collection | DOAJ |
| description | Intestinal bacteria play important roles in the progression of colitis-associated carcinogenesis. Colostrum-derived <i>Lacticaseibacillus rhamnosus</i> Probio-M9 (Probio-M9) has shown a protective effect in a colitis-associated cancer (CAC) model, but detailed metagenomic analysis had not been performed. Here, we investigated the preventive effects of the probiotic Probio-M9 on CAC-model mice, tracking the microbiota. Feces were obtained at four time points for evaluation of gut microbiota. The effect of Probio-M9 on tight junction protein expression was evaluated in co-cultured Caco-2 cells. Probio-M9 treatment decreased the number of tumors as well as stool consistency score, spleen weight, inflammatory score, and macrophage expression in the CAC model. Probio-M9 accelerated the recovery of the structure, composition, and function of the intestinal microbiota destroyed by azoxymethane (AOM)/dextran sulfate sodium (DSS) by regulating key bacteria (including <i>Lactobacillus murinus</i>, <i>Muribaculaceae bacterium</i> DSM 103720, <i>Muribaculum intestinale</i>, and <i>Lachnospiraceae bacterium</i> A4) and pathways from immediately after administration until the end of the experiment. Probio-M9 co-culture protected against lipopolysaccharide-induced impairment of tight junctions in Caco-2 cells. This study provides valuable insight into the role of Probio-M9 in correcting gut microbiota defects associated with inflammatory bowel disease carcinogenesis and may have clinical application in the treatment of inflammatory carcinogenesis. |
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| spelling | doaj.art-9a794328b85b42c08c7445593a98e1c22024-03-27T13:22:43ZengMDPI AGBiomedicines2227-90592024-02-0112353110.3390/biomedicines12030531Long-Term Tracking of the Effects of Colostrum-Derived <i>Lacticaseibacillus rhamnosus</i> Probio-M9 on Gut Microbiota in Mice with Colitis-Associated TumorigenesisFeiyan Zhao0Keizo Hiraishi1Xiaodong Li2Yaopeng Hu3Daibo Kojima4Zhihong Sun5Heping Zhang6Lin-Hai Kurahara7Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, ChinaDepartment of Cardiovascular Physiology, Faculty of Medicine, Kagawa University, Takamatsu 761-0793, Kagawa, JapanDepartment of Cardiovascular Physiology, Faculty of Medicine, Kagawa University, Takamatsu 761-0793, Kagawa, JapanDepartment of Physiology, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, Fukuoka, JapanDepartment of Gastroenterological Surgery, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, Fukuoka, JapanInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, ChinaDepartment of Cardiovascular Physiology, Faculty of Medicine, Kagawa University, Takamatsu 761-0793, Kagawa, JapanIntestinal bacteria play important roles in the progression of colitis-associated carcinogenesis. Colostrum-derived <i>Lacticaseibacillus rhamnosus</i> Probio-M9 (Probio-M9) has shown a protective effect in a colitis-associated cancer (CAC) model, but detailed metagenomic analysis had not been performed. Here, we investigated the preventive effects of the probiotic Probio-M9 on CAC-model mice, tracking the microbiota. Feces were obtained at four time points for evaluation of gut microbiota. The effect of Probio-M9 on tight junction protein expression was evaluated in co-cultured Caco-2 cells. Probio-M9 treatment decreased the number of tumors as well as stool consistency score, spleen weight, inflammatory score, and macrophage expression in the CAC model. Probio-M9 accelerated the recovery of the structure, composition, and function of the intestinal microbiota destroyed by azoxymethane (AOM)/dextran sulfate sodium (DSS) by regulating key bacteria (including <i>Lactobacillus murinus</i>, <i>Muribaculaceae bacterium</i> DSM 103720, <i>Muribaculum intestinale</i>, and <i>Lachnospiraceae bacterium</i> A4) and pathways from immediately after administration until the end of the experiment. Probio-M9 co-culture protected against lipopolysaccharide-induced impairment of tight junctions in Caco-2 cells. This study provides valuable insight into the role of Probio-M9 in correcting gut microbiota defects associated with inflammatory bowel disease carcinogenesis and may have clinical application in the treatment of inflammatory carcinogenesis.https://www.mdpi.com/2227-9059/12/3/531inflammatory bowel diseasecolitis-associated carcinogenesiscolorectal cancerprobioticsbreastfeeding |
| spellingShingle | Feiyan Zhao Keizo Hiraishi Xiaodong Li Yaopeng Hu Daibo Kojima Zhihong Sun Heping Zhang Lin-Hai Kurahara Long-Term Tracking of the Effects of Colostrum-Derived <i>Lacticaseibacillus rhamnosus</i> Probio-M9 on Gut Microbiota in Mice with Colitis-Associated Tumorigenesis Biomedicines inflammatory bowel disease colitis-associated carcinogenesis colorectal cancer probiotics breastfeeding |
| title | Long-Term Tracking of the Effects of Colostrum-Derived <i>Lacticaseibacillus rhamnosus</i> Probio-M9 on Gut Microbiota in Mice with Colitis-Associated Tumorigenesis |
| title_full | Long-Term Tracking of the Effects of Colostrum-Derived <i>Lacticaseibacillus rhamnosus</i> Probio-M9 on Gut Microbiota in Mice with Colitis-Associated Tumorigenesis |
| title_fullStr | Long-Term Tracking of the Effects of Colostrum-Derived <i>Lacticaseibacillus rhamnosus</i> Probio-M9 on Gut Microbiota in Mice with Colitis-Associated Tumorigenesis |
| title_full_unstemmed | Long-Term Tracking of the Effects of Colostrum-Derived <i>Lacticaseibacillus rhamnosus</i> Probio-M9 on Gut Microbiota in Mice with Colitis-Associated Tumorigenesis |
| title_short | Long-Term Tracking of the Effects of Colostrum-Derived <i>Lacticaseibacillus rhamnosus</i> Probio-M9 on Gut Microbiota in Mice with Colitis-Associated Tumorigenesis |
| title_sort | long term tracking of the effects of colostrum derived i lacticaseibacillus rhamnosus i probio m9 on gut microbiota in mice with colitis associated tumorigenesis |
| topic | inflammatory bowel disease colitis-associated carcinogenesis colorectal cancer probiotics breastfeeding |
| url | https://www.mdpi.com/2227-9059/12/3/531 |
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