Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues
The endogenous fluorophores such as reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) in cells and tissues can be imaged by fluorescence lifetime imaging microscopy (FLIM) to show the tissue morphology features, as well as the biomolecular changes...
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2019-09-01
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| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | http://www.worldscientific.com/doi/pdf/10.1142/S1793545819400066 |
| _version_ | 1818038046379999232 |
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| author | Maojia Huang Zixiao Zhang Xinyi Wang Yonghui Xie Yiyan Fei Jiong Ma Jing Wang Li Chen Lan Mi Yulan Wang |
| author_facet | Maojia Huang Zixiao Zhang Xinyi Wang Yonghui Xie Yiyan Fei Jiong Ma Jing Wang Li Chen Lan Mi Yulan Wang |
| author_sort | Maojia Huang |
| collection | DOAJ |
| description | The endogenous fluorophores such as reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) in cells and tissues can be imaged by fluorescence lifetime imaging microscopy (FLIM) to show the tissue morphology features, as well as the biomolecular changes in microenvironment. The two important coenzymes in cellular metabolism, NAD(P)H and FAD, can be used to monitor the cellular metabolic status. This work proposed a novel method to study the uterine metabolism at the adjacent site of healthy cervix. It was found that the benign uterine tumors such as leiomyomas and adenomyosis with abnormal cell growth can be detected by measuring the fluorescence lifetime of NAD(P)H and FAD in adjacent healthy cervical tissues. This method opened a novel strategy for afflicted women to undergo the cervical biopsies instead of hysterectomies for detecting tumors, which can preserve the fertility of patients. The FLIM studying on NAD(P)H and FAD indicated the correlation between metabolism and some diseases, including diabetes, hyperthyroidism and obesity. It was also suggested that the metabolic level might be quite different for a patient with a malignant tumor history. |
| first_indexed | 2024-12-10T07:36:31Z |
| format | Article |
| id | doaj.art-9a79901cafce4dacbacd8ed495e91e6a |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-12-10T07:36:31Z |
| publishDate | 2019-09-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-9a79901cafce4dacbacd8ed495e91e6a2022-12-22T01:57:24ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052019-09-011251940006-11940006-1110.1142/S179354581940006610.1142/S1793545819400066Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissuesMaojia Huang0Zixiao Zhang1Xinyi Wang2Yonghui Xie3Yiyan Fei4Jiong Ma5Jing Wang6Li Chen7Lan Mi8Yulan Wang9Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Fudan University, Shanghai 200433, P. R. ChinaDepartment of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Fudan University, Shanghai 200433, P. R. ChinaDepartment of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Fudan University, Shanghai 200433, P. R. ChinaDepartment of Pathology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, P. R. ChinaDepartment of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Fudan University, Shanghai 200433, P. R. ChinaDepartment of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Fudan University, Shanghai 200433, P. R. ChinaState Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, P. R. ChinaSchool of Arts and Sciences, MCPHS University, Boston, MA 02115, U. S. A.Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Fudan University, Shanghai 200433, P. R. ChinaDepartment of Gynecology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, P. R. ChinaThe endogenous fluorophores such as reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) in cells and tissues can be imaged by fluorescence lifetime imaging microscopy (FLIM) to show the tissue morphology features, as well as the biomolecular changes in microenvironment. The two important coenzymes in cellular metabolism, NAD(P)H and FAD, can be used to monitor the cellular metabolic status. This work proposed a novel method to study the uterine metabolism at the adjacent site of healthy cervix. It was found that the benign uterine tumors such as leiomyomas and adenomyosis with abnormal cell growth can be detected by measuring the fluorescence lifetime of NAD(P)H and FAD in adjacent healthy cervical tissues. This method opened a novel strategy for afflicted women to undergo the cervical biopsies instead of hysterectomies for detecting tumors, which can preserve the fertility of patients. The FLIM studying on NAD(P)H and FAD indicated the correlation between metabolism and some diseases, including diabetes, hyperthyroidism and obesity. It was also suggested that the metabolic level might be quite different for a patient with a malignant tumor history.http://www.worldscientific.com/doi/pdf/10.1142/S1793545819400066Autofluorescencefluorescence lifetime imagingbenign uterine tumormetabolism |
| spellingShingle | Maojia Huang Zixiao Zhang Xinyi Wang Yonghui Xie Yiyan Fei Jiong Ma Jing Wang Li Chen Lan Mi Yulan Wang Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues Journal of Innovative Optical Health Sciences Autofluorescence fluorescence lifetime imaging benign uterine tumor metabolism |
| title | Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues |
| title_full | Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues |
| title_fullStr | Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues |
| title_full_unstemmed | Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues |
| title_short | Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues |
| title_sort | detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues |
| topic | Autofluorescence fluorescence lifetime imaging benign uterine tumor metabolism |
| url | http://www.worldscientific.com/doi/pdf/10.1142/S1793545819400066 |
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