ABTS On-Line Antioxidant, α-Amylase, α-Glucosidase, Pancreatic Lipase, Acetyl- and Butyrylcholinesterase Inhibition Activity of <i>Chaenomeles</i> Fruits Determined by Polyphenols and other Chemical Compounds
This study aimed to identify and quantify the chemical composition and polyphenolic profile of 19 cultivars of <i>Chaenomeles × superba</i>, <i>Chaenomeles japonic</i>a, and <i>Chaenomeles speciosa</i> by liquid chromatography coupled with photodiode arra...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/1/60 |
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| author | Igor Piotr Turkiewicz Aneta Wojdyło Karolina Tkacz Paulina Nowicka Tomasz Golis Przemysław Bąbelewski |
| author_facet | Igor Piotr Turkiewicz Aneta Wojdyło Karolina Tkacz Paulina Nowicka Tomasz Golis Przemysław Bąbelewski |
| author_sort | Igor Piotr Turkiewicz |
| collection | DOAJ |
| description | This study aimed to identify and quantify the chemical composition and polyphenolic profile of 19 cultivars of <i>Chaenomeles × superba</i>, <i>Chaenomeles japonic</i>a, and <i>Chaenomeles speciosa</i> by liquid chromatography coupled with photodiode array detector and quadrupole time-of-flight electrospray ionization mass spectrometry (LC-PDA-QTOF-ESI-MS). Antioxidant (ABTS on-line, ABTS, FRAP, and ORAC), as well as in vitro biological activities, i.e., the ability to inhibit α-amylase, α-glucosidase, pancreatic lipase, acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), and 15-lipoxygenase (15-LOX) were determined. Most of the <i>Chaenomeles</i> species and cultivars analyzed in this study have not been examined in this respect until now. Fruits contained 30.26 to 195.05 mg of vitamin C, 0.65 to 1.69 g of pectin, 0.32 to 0.64 g of ash, 0.60 to 3.98 g of sugars, and 41.64 to 110.31 g of organic acids in 100 g fresh weight. The lowest content of total polyphenols showed <i>C. speciosa</i> ‘Rubra’ (57.84 g/kg dry weight, dw) while <i>C.</i> × <i>superba</i> ’Nicoline’ (170.38 g/kg dw) exhibited the highest concentration of those compounds. In the phenolic compounds, polymeric procyanidin fraction predominated (65%) with procyanidin B2, C1, and (−)-epicatechin the most abundant. The antioxidant capacity measured by ABTS assay was mainly formed by polymeric procyanidins and flavan-3-ols, which was confirmed by ABTS on-line profiling. <i>Chaenomeles</i> fruits showed high potential for inhibition of α-glucosidase and pancreatic lipase. The analyzed cultivars displayed greater potential for acetylcholinesterase (AChE) inhibition than for butyrylcholinesterase (BuChE). The data indicate that <i>Chaenomeles</i> fruits could be regarded as a promising source of bioactive functional food. |
| first_indexed | 2024-03-12T07:39:36Z |
| format | Article |
| id | doaj.art-9a7e2db92e6d42de94d7e760b6c58761 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T07:39:36Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-9a7e2db92e6d42de94d7e760b6c587612023-09-02T21:21:43ZengMDPI AGAntioxidants2076-39212020-01-01916010.3390/antiox9010060antiox9010060ABTS On-Line Antioxidant, α-Amylase, α-Glucosidase, Pancreatic Lipase, Acetyl- and Butyrylcholinesterase Inhibition Activity of <i>Chaenomeles</i> Fruits Determined by Polyphenols and other Chemical CompoundsIgor Piotr Turkiewicz0Aneta Wojdyło1Karolina Tkacz2Paulina Nowicka3Tomasz Golis4Przemysław Bąbelewski5Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Sciences, 37 Chełmońskiego Str., 51-630 Wrocław, PolandDepartment of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Sciences, 37 Chełmońskiego Str., 51-630 Wrocław, PolandDepartment of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Sciences, 37 Chełmońskiego Str., 51-630 Wrocław, PolandDepartment of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Sciences, 37 Chełmońskiego Str., 51-630 Wrocław, PolandResearch Institute of Horticulture, Department of Pomology, Gene Resources and Nurseries, 1/3 Konstytucji 3 Maja Str., 96-100 Skierniewice, PolandDepartment of Horticulture, Wrocław University of Environmental and Life Sciences, 24a Grunwaldzki Str., 53-363 Wrocław, PolandThis study aimed to identify and quantify the chemical composition and polyphenolic profile of 19 cultivars of <i>Chaenomeles × superba</i>, <i>Chaenomeles japonic</i>a, and <i>Chaenomeles speciosa</i> by liquid chromatography coupled with photodiode array detector and quadrupole time-of-flight electrospray ionization mass spectrometry (LC-PDA-QTOF-ESI-MS). Antioxidant (ABTS on-line, ABTS, FRAP, and ORAC), as well as in vitro biological activities, i.e., the ability to inhibit α-amylase, α-glucosidase, pancreatic lipase, acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), and 15-lipoxygenase (15-LOX) were determined. Most of the <i>Chaenomeles</i> species and cultivars analyzed in this study have not been examined in this respect until now. Fruits contained 30.26 to 195.05 mg of vitamin C, 0.65 to 1.69 g of pectin, 0.32 to 0.64 g of ash, 0.60 to 3.98 g of sugars, and 41.64 to 110.31 g of organic acids in 100 g fresh weight. The lowest content of total polyphenols showed <i>C. speciosa</i> ‘Rubra’ (57.84 g/kg dry weight, dw) while <i>C.</i> × <i>superba</i> ’Nicoline’ (170.38 g/kg dw) exhibited the highest concentration of those compounds. In the phenolic compounds, polymeric procyanidin fraction predominated (65%) with procyanidin B2, C1, and (−)-epicatechin the most abundant. The antioxidant capacity measured by ABTS assay was mainly formed by polymeric procyanidins and flavan-3-ols, which was confirmed by ABTS on-line profiling. <i>Chaenomeles</i> fruits showed high potential for inhibition of α-glucosidase and pancreatic lipase. The analyzed cultivars displayed greater potential for acetylcholinesterase (AChE) inhibition than for butyrylcholinesterase (BuChE). The data indicate that <i>Chaenomeles</i> fruits could be regarded as a promising source of bioactive functional food.https://www.mdpi.com/2076-3921/9/1/60japanese quinceflowering quincelc-pda-qtof-esi-msα-amylaseα-glucosidasepancreatic lipaseachebuche15-loxantioxidant capacityabts on-lineahc analysis |
| spellingShingle | Igor Piotr Turkiewicz Aneta Wojdyło Karolina Tkacz Paulina Nowicka Tomasz Golis Przemysław Bąbelewski ABTS On-Line Antioxidant, α-Amylase, α-Glucosidase, Pancreatic Lipase, Acetyl- and Butyrylcholinesterase Inhibition Activity of <i>Chaenomeles</i> Fruits Determined by Polyphenols and other Chemical Compounds Antioxidants japanese quince flowering quince lc-pda-qtof-esi-ms α-amylase α-glucosidase pancreatic lipase ache buche 15-lox antioxidant capacity abts on-line ahc analysis |
| title | ABTS On-Line Antioxidant, α-Amylase, α-Glucosidase, Pancreatic Lipase, Acetyl- and Butyrylcholinesterase Inhibition Activity of <i>Chaenomeles</i> Fruits Determined by Polyphenols and other Chemical Compounds |
| title_full | ABTS On-Line Antioxidant, α-Amylase, α-Glucosidase, Pancreatic Lipase, Acetyl- and Butyrylcholinesterase Inhibition Activity of <i>Chaenomeles</i> Fruits Determined by Polyphenols and other Chemical Compounds |
| title_fullStr | ABTS On-Line Antioxidant, α-Amylase, α-Glucosidase, Pancreatic Lipase, Acetyl- and Butyrylcholinesterase Inhibition Activity of <i>Chaenomeles</i> Fruits Determined by Polyphenols and other Chemical Compounds |
| title_full_unstemmed | ABTS On-Line Antioxidant, α-Amylase, α-Glucosidase, Pancreatic Lipase, Acetyl- and Butyrylcholinesterase Inhibition Activity of <i>Chaenomeles</i> Fruits Determined by Polyphenols and other Chemical Compounds |
| title_short | ABTS On-Line Antioxidant, α-Amylase, α-Glucosidase, Pancreatic Lipase, Acetyl- and Butyrylcholinesterase Inhibition Activity of <i>Chaenomeles</i> Fruits Determined by Polyphenols and other Chemical Compounds |
| title_sort | abts on line antioxidant α amylase α glucosidase pancreatic lipase acetyl and butyrylcholinesterase inhibition activity of i chaenomeles i fruits determined by polyphenols and other chemical compounds |
| topic | japanese quince flowering quince lc-pda-qtof-esi-ms α-amylase α-glucosidase pancreatic lipase ache buche 15-lox antioxidant capacity abts on-line ahc analysis |
| url | https://www.mdpi.com/2076-3921/9/1/60 |
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