Interactions between intracellular Ca2+ stores: Ca2+ released from the NAADP pool potentiates cADPR-induced Ca2+ release
Cells possess multiple intracellular Ca2+-releasing systems. Sea urchin egg homogenates are a well-established model to study intracellular Ca2+ release. In the present study the mechanism of interaction between three intracellular Ca2+ pools, namely the nicotinic acid adenine dinucleotide phosphate...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2002-05-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500005 |
| _version_ | 1818067436290703360 |
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| author | E.N. Chini |
| author_facet | E.N. Chini |
| author_sort | E.N. Chini |
| collection | DOAJ |
| description | Cells possess multiple intracellular Ca2+-releasing systems. Sea urchin egg homogenates are a well-established model to study intracellular Ca2+ release. In the present study the mechanism of interaction between three intracellular Ca2+ pools, namely the nicotinic acid adenine dinucleotide phosphate (NAADP), the cyclic ADP-ribose (cADPR) and the inositol 1',4',5'-trisphosphate (IP3)-regulated Ca2+ stores, is explored. The data indicate that the NAADP Ca2+ pool could be used to sensitize the cADPR system. In contrast, the IP3 pool was not affected by the Ca2+ released by NAADP. The mechanism of potentiation of the cADPR-induced Ca2+ release, promoted by Ca2+ released from the NAADP pool, is mediated by the mechanism of Ca2+-induced Ca2+ release. These data raise the possibility that the NAADP Ca2+ store may have a role as a regulator of the cellular sensitivity to cADPR. |
| first_indexed | 2024-12-10T15:23:39Z |
| format | Article |
| id | doaj.art-9a81d65f8bc74c49aee3c350319f0cfb |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-10T15:23:39Z |
| publishDate | 2002-05-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-9a81d65f8bc74c49aee3c350319f0cfb2022-12-22T01:43:36ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2002-05-0135554354710.1590/S0100-879X2002000500005Interactions between intracellular Ca2+ stores: Ca2+ released from the NAADP pool potentiates cADPR-induced Ca2+ releaseE.N. ChiniCells possess multiple intracellular Ca2+-releasing systems. Sea urchin egg homogenates are a well-established model to study intracellular Ca2+ release. In the present study the mechanism of interaction between three intracellular Ca2+ pools, namely the nicotinic acid adenine dinucleotide phosphate (NAADP), the cyclic ADP-ribose (cADPR) and the inositol 1',4',5'-trisphosphate (IP3)-regulated Ca2+ stores, is explored. The data indicate that the NAADP Ca2+ pool could be used to sensitize the cADPR system. In contrast, the IP3 pool was not affected by the Ca2+ released by NAADP. The mechanism of potentiation of the cADPR-induced Ca2+ release, promoted by Ca2+ released from the NAADP pool, is mediated by the mechanism of Ca2+-induced Ca2+ release. These data raise the possibility that the NAADP Ca2+ store may have a role as a regulator of the cellular sensitivity to cADPR.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500005cADPRNAADPCalciumSea urchin eggsFertilizationIP3 |
| spellingShingle | E.N. Chini Interactions between intracellular Ca2+ stores: Ca2+ released from the NAADP pool potentiates cADPR-induced Ca2+ release Brazilian Journal of Medical and Biological Research cADPR NAADP Calcium Sea urchin eggs Fertilization IP3 |
| title | Interactions between intracellular Ca2+ stores: Ca2+ released from the NAADP pool potentiates cADPR-induced Ca2+ release |
| title_full | Interactions between intracellular Ca2+ stores: Ca2+ released from the NAADP pool potentiates cADPR-induced Ca2+ release |
| title_fullStr | Interactions between intracellular Ca2+ stores: Ca2+ released from the NAADP pool potentiates cADPR-induced Ca2+ release |
| title_full_unstemmed | Interactions between intracellular Ca2+ stores: Ca2+ released from the NAADP pool potentiates cADPR-induced Ca2+ release |
| title_short | Interactions between intracellular Ca2+ stores: Ca2+ released from the NAADP pool potentiates cADPR-induced Ca2+ release |
| title_sort | interactions between intracellular ca2 stores ca2 released from the naadp pool potentiates cadpr induced ca2 release |
| topic | cADPR NAADP Calcium Sea urchin eggs Fertilization IP3 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500005 |
| work_keys_str_mv | AT enchini interactionsbetweenintracellularca2storesca2releasedfromthenaadppoolpotentiatescadprinducedca2release |