How <i>Pseudomonas nitroreducens</i> Passivates Cadmium to Inhibit Plant Uptake
Cadmium (Cd) has been widely used in industry applications, leading to water and soil contamination. This study investigated the potential ability of <i>Pseudomonas nitroreducens</i> (11830) to perform the biosorption of cadmium from aqueous solution and soil. The biosorption characteris...
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MDPI AG
2024-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/14/7/2857 |
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| author | Yakui Chen Yongquan Yu Xiaoyu Fang Yinhuan Zhou Diannan Lu |
| author_facet | Yakui Chen Yongquan Yu Xiaoyu Fang Yinhuan Zhou Diannan Lu |
| author_sort | Yakui Chen |
| collection | DOAJ |
| description | Cadmium (Cd) has been widely used in industry applications, leading to water and soil contamination. This study investigated the potential ability of <i>Pseudomonas nitroreducens</i> (11830) to perform the biosorption of cadmium from aqueous solution and soil. The biosorption characteristics were described using equilibrium isotherm and kinetic studies. The Langmuir adsorption isotherm indicated a better fit with the experimental data (R<sup>2</sup> = 0.980), with a maximum capacity of 160.51 mg/g at 30 °C in an initial aqueous solution of 300 mg/L Cd<sup>2+</sup>. The experiments followed a pseudo-second-order kinetics model (R<sup>2</sup> > 0.99), especially at a low initial concentration. The biosorption mechanisms involved were determined through scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDS) and protein analysis. The SEM and TEM figures showed that the morphology of cells changed before and after the adsorption of Cd, and the EDS confirmed that Cd was absorbed on the surface of the cell. The analysis of proteins indicated that the protein species increased after the stimulation of Cd, which further confirmed the biosorption mechanism. A pot experiment confirmed that 11830 could passivate the cadmium in soil and reduce its uptake and utilization by <i>Houttuynia cordata Thunb</i> (<i>H. cordata</i>). This work demonstrates the potential application of microorganisms in inhibiting the accumulation of Cd in crops. |
| first_indexed | 2024-04-24T10:50:09Z |
| format | Article |
| id | doaj.art-9a87e5dc25dc4eb7bf6ab54277ece15a |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-04-24T10:50:09Z |
| publishDate | 2024-03-01 |
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| series | Applied Sciences |
| spelling | doaj.art-9a87e5dc25dc4eb7bf6ab54277ece15a2024-04-12T13:15:01ZengMDPI AGApplied Sciences2076-34172024-03-01147285710.3390/app14072857How <i>Pseudomonas nitroreducens</i> Passivates Cadmium to Inhibit Plant UptakeYakui Chen0Yongquan Yu1Xiaoyu Fang2Yinhuan Zhou3Diannan Lu4Department of Chemical Engineering, Tsinghua University, Beijing 100084, ChinaDepartment of Chemical Engineering, Tsinghua University, Beijing 100084, ChinaDepartment of Chemical Engineering, Tsinghua University, Beijing 100084, ChinaDepartment of Chemical Engineering, Tsinghua University, Beijing 100084, ChinaDepartment of Chemical Engineering, Tsinghua University, Beijing 100084, ChinaCadmium (Cd) has been widely used in industry applications, leading to water and soil contamination. This study investigated the potential ability of <i>Pseudomonas nitroreducens</i> (11830) to perform the biosorption of cadmium from aqueous solution and soil. The biosorption characteristics were described using equilibrium isotherm and kinetic studies. The Langmuir adsorption isotherm indicated a better fit with the experimental data (R<sup>2</sup> = 0.980), with a maximum capacity of 160.51 mg/g at 30 °C in an initial aqueous solution of 300 mg/L Cd<sup>2+</sup>. The experiments followed a pseudo-second-order kinetics model (R<sup>2</sup> > 0.99), especially at a low initial concentration. The biosorption mechanisms involved were determined through scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDS) and protein analysis. The SEM and TEM figures showed that the morphology of cells changed before and after the adsorption of Cd, and the EDS confirmed that Cd was absorbed on the surface of the cell. The analysis of proteins indicated that the protein species increased after the stimulation of Cd, which further confirmed the biosorption mechanism. A pot experiment confirmed that 11830 could passivate the cadmium in soil and reduce its uptake and utilization by <i>Houttuynia cordata Thunb</i> (<i>H. cordata</i>). This work demonstrates the potential application of microorganisms in inhibiting the accumulation of Cd in crops.https://www.mdpi.com/2076-3417/14/7/2857<i>Pseudomonas nitroreducens</i>heavy metal cadmiumbioaccumulationbiosorptionbiosorption mechanism |
| spellingShingle | Yakui Chen Yongquan Yu Xiaoyu Fang Yinhuan Zhou Diannan Lu How <i>Pseudomonas nitroreducens</i> Passivates Cadmium to Inhibit Plant Uptake Applied Sciences <i>Pseudomonas nitroreducens</i> heavy metal cadmium bioaccumulation biosorption biosorption mechanism |
| title | How <i>Pseudomonas nitroreducens</i> Passivates Cadmium to Inhibit Plant Uptake |
| title_full | How <i>Pseudomonas nitroreducens</i> Passivates Cadmium to Inhibit Plant Uptake |
| title_fullStr | How <i>Pseudomonas nitroreducens</i> Passivates Cadmium to Inhibit Plant Uptake |
| title_full_unstemmed | How <i>Pseudomonas nitroreducens</i> Passivates Cadmium to Inhibit Plant Uptake |
| title_short | How <i>Pseudomonas nitroreducens</i> Passivates Cadmium to Inhibit Plant Uptake |
| title_sort | how i pseudomonas nitroreducens i passivates cadmium to inhibit plant uptake |
| topic | <i>Pseudomonas nitroreducens</i> heavy metal cadmium bioaccumulation biosorption biosorption mechanism |
| url | https://www.mdpi.com/2076-3417/14/7/2857 |
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