A New Assay for Measurement of Acetylcholinesterase and Butyrylcholinesterase in Canine Whole Blood Combining Specific Substrates and Ethopropazine Hydrochloride as a Selective Butyrylcholinesterase Inhibitor
In the present report, a new assay combining specific substrates and a selective BChE inhibitor (ethopropazine hydrochloride) was used to measure both AChE and BChE in canine whole blood samples. Acetylthiocholine iodide (ATCI) and butyrylthiocholine iodide (BTCI) were used as substrates, whereas 2,...
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| Format: | Article |
| Language: | English |
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University of Agriculture, Faisalabad
2013-11-01
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| Series: | Pakistan Veterinary Journal |
| Subjects: | |
| Online Access: | http://pvj.com.pk/pdf-files/33_4/458-461.pdf |
| _version_ | 1818439575166517248 |
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| author | F Tecles, A Tvarijonaviciute and JJ Cerón* |
| author_facet | F Tecles, A Tvarijonaviciute and JJ Cerón* |
| author_sort | F Tecles, A Tvarijonaviciute and JJ Cerón* |
| collection | DOAJ |
| description | In the present report, a new assay combining specific substrates and a selective BChE inhibitor (ethopropazine hydrochloride) was used to measure both AChE and BChE in canine whole blood samples. Acetylthiocholine iodide (ATCI) and butyrylthiocholine iodide (BTCI) were used as substrates, whereas 2,2’-dithiodipiridine was used as chromophore. Ethopropazine concentration inhibiting over 95% BChE with minimum AChE inhibition was fixed at 0.3mM. The results confirmed that whole blood cholinesterase activity measured with BTCI in absence of ethopropazine corresponded with serum BChE, whereas whole blood cholinesterase analysed with ATCI in presence of ethopropazine reflected mainly erythrocytes and plasma AChE activity. This procedure showed good repeatability, it was easy and fast, and can be routinely used in veterinary laboratories. |
| first_indexed | 2024-12-14T17:58:38Z |
| format | Article |
| id | doaj.art-9a88610393084df7b3841a8bae1678aa |
| institution | Directory Open Access Journal |
| issn | 0253-8318 2074-7764 |
| language | English |
| last_indexed | 2024-12-14T17:58:38Z |
| publishDate | 2013-11-01 |
| publisher | University of Agriculture, Faisalabad |
| record_format | Article |
| series | Pakistan Veterinary Journal |
| spelling | doaj.art-9a88610393084df7b3841a8bae1678aa2022-12-21T22:52:30ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182074-77642013-11-01334458461A New Assay for Measurement of Acetylcholinesterase and Butyrylcholinesterase in Canine Whole Blood Combining Specific Substrates and Ethopropazine Hydrochloride as a Selective Butyrylcholinesterase InhibitorF Tecles, A Tvarijonaviciute and JJ Cerón*In the present report, a new assay combining specific substrates and a selective BChE inhibitor (ethopropazine hydrochloride) was used to measure both AChE and BChE in canine whole blood samples. Acetylthiocholine iodide (ATCI) and butyrylthiocholine iodide (BTCI) were used as substrates, whereas 2,2’-dithiodipiridine was used as chromophore. Ethopropazine concentration inhibiting over 95% BChE with minimum AChE inhibition was fixed at 0.3mM. The results confirmed that whole blood cholinesterase activity measured with BTCI in absence of ethopropazine corresponded with serum BChE, whereas whole blood cholinesterase analysed with ATCI in presence of ethopropazine reflected mainly erythrocytes and plasma AChE activity. This procedure showed good repeatability, it was easy and fast, and can be routinely used in veterinary laboratories.http://pvj.com.pk/pdf-files/33_4/458-461.pdfAcetylcholinesteraseButyrylcholinesteraseDogEthopropazineSubstrateWhole blood |
| spellingShingle | F Tecles, A Tvarijonaviciute and JJ Cerón* A New Assay for Measurement of Acetylcholinesterase and Butyrylcholinesterase in Canine Whole Blood Combining Specific Substrates and Ethopropazine Hydrochloride as a Selective Butyrylcholinesterase Inhibitor Pakistan Veterinary Journal Acetylcholinesterase Butyrylcholinesterase Dog Ethopropazine Substrate Whole blood |
| title | A New Assay for Measurement of Acetylcholinesterase and Butyrylcholinesterase in Canine Whole Blood Combining Specific Substrates and Ethopropazine Hydrochloride as a Selective Butyrylcholinesterase Inhibitor |
| title_full | A New Assay for Measurement of Acetylcholinesterase and Butyrylcholinesterase in Canine Whole Blood Combining Specific Substrates and Ethopropazine Hydrochloride as a Selective Butyrylcholinesterase Inhibitor |
| title_fullStr | A New Assay for Measurement of Acetylcholinesterase and Butyrylcholinesterase in Canine Whole Blood Combining Specific Substrates and Ethopropazine Hydrochloride as a Selective Butyrylcholinesterase Inhibitor |
| title_full_unstemmed | A New Assay for Measurement of Acetylcholinesterase and Butyrylcholinesterase in Canine Whole Blood Combining Specific Substrates and Ethopropazine Hydrochloride as a Selective Butyrylcholinesterase Inhibitor |
| title_short | A New Assay for Measurement of Acetylcholinesterase and Butyrylcholinesterase in Canine Whole Blood Combining Specific Substrates and Ethopropazine Hydrochloride as a Selective Butyrylcholinesterase Inhibitor |
| title_sort | new assay for measurement of acetylcholinesterase and butyrylcholinesterase in canine whole blood combining specific substrates and ethopropazine hydrochloride as a selective butyrylcholinesterase inhibitor |
| topic | Acetylcholinesterase Butyrylcholinesterase Dog Ethopropazine Substrate Whole blood |
| url | http://pvj.com.pk/pdf-files/33_4/458-461.pdf |
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