Molecular Characterization and Expression Pattern of Paramyosin in Larvae and Adults of Yesso Scallop
Paramyosin is an important myofibrillar protein in molluscan smooth muscle. The full-length cDNA encoding paramyosin has been identified from Yesso scallop <i>Patinopecten yessoensis</i>. The length of paramyosin molecule has been found to be 3715 bp, which contains an open reading frame...
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/3/453 |
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| author | Yumin Yang Dan Zhao Liqing Zhou Tianshi Zhang Zhihong Liu Biao Wu Tao Yu Yanxin Zheng Xiujun Sun |
| author_facet | Yumin Yang Dan Zhao Liqing Zhou Tianshi Zhang Zhihong Liu Biao Wu Tao Yu Yanxin Zheng Xiujun Sun |
| author_sort | Yumin Yang |
| collection | DOAJ |
| description | Paramyosin is an important myofibrillar protein in molluscan smooth muscle. The full-length cDNA encoding paramyosin has been identified from Yesso scallop <i>Patinopecten yessoensis</i>. The length of paramyosin molecule has been found to be 3715 bp, which contains an open reading frame (ORF) of 2805 bp for 934 amino acid residues. Characterization of <i>P. yessoensis</i> paramyosin reveals the typical structural feature of coiled-coil protein, including six α-helix (α1-α6) and one coil (η) structures. Multiple phosphorylation sites have been predicted at the N-terminus of paramyosin, representing the unique phosphorylation sites in scallops. The highest levels of mRNA and protein expression of paramyosin have been found in foot and the smooth adductor muscle. According to whole-mount in situ hybridization (WISH), strong paramyosin mRNA signals were detected in the symmetric positions of anterior and posterior adductor muscles at late larval stages. These findings support that paramyosin may serve as the most important components for myogenesis and catch regulation in scallops. The present findings will not only help uncover the potential function of myofibrillar proteins in molluscs but also provide molecular evidence to infer evolutionary relationships among invertebrates. |
| first_indexed | 2024-03-09T13:49:19Z |
| format | Article |
| id | doaj.art-9a88895c773842d0ab7bad49d3311c96 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T13:49:19Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-9a88895c773842d0ab7bad49d3311c962023-11-30T20:52:25ZengMDPI AGBiology2079-77372022-03-0111345310.3390/biology11030453Molecular Characterization and Expression Pattern of Paramyosin in Larvae and Adults of Yesso ScallopYumin Yang0Dan Zhao1Liqing Zhou2Tianshi Zhang3Zhihong Liu4Biao Wu5Tao Yu6Yanxin Zheng7Xiujun Sun8Fisheries College, Zhejiang Ocean University, Zhoushan 316022, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaChangdao Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Yantai 265800, ChinaChangdao Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Yantai 265800, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaParamyosin is an important myofibrillar protein in molluscan smooth muscle. The full-length cDNA encoding paramyosin has been identified from Yesso scallop <i>Patinopecten yessoensis</i>. The length of paramyosin molecule has been found to be 3715 bp, which contains an open reading frame (ORF) of 2805 bp for 934 amino acid residues. Characterization of <i>P. yessoensis</i> paramyosin reveals the typical structural feature of coiled-coil protein, including six α-helix (α1-α6) and one coil (η) structures. Multiple phosphorylation sites have been predicted at the N-terminus of paramyosin, representing the unique phosphorylation sites in scallops. The highest levels of mRNA and protein expression of paramyosin have been found in foot and the smooth adductor muscle. According to whole-mount in situ hybridization (WISH), strong paramyosin mRNA signals were detected in the symmetric positions of anterior and posterior adductor muscles at late larval stages. These findings support that paramyosin may serve as the most important components for myogenesis and catch regulation in scallops. The present findings will not only help uncover the potential function of myofibrillar proteins in molluscs but also provide molecular evidence to infer evolutionary relationships among invertebrates.https://www.mdpi.com/2079-7737/11/3/453scallopssmooth adductor muscleparamyosingene expressionwhole-mount in situ hybridization |
| spellingShingle | Yumin Yang Dan Zhao Liqing Zhou Tianshi Zhang Zhihong Liu Biao Wu Tao Yu Yanxin Zheng Xiujun Sun Molecular Characterization and Expression Pattern of Paramyosin in Larvae and Adults of Yesso Scallop Biology scallops smooth adductor muscle paramyosin gene expression whole-mount in situ hybridization |
| title | Molecular Characterization and Expression Pattern of Paramyosin in Larvae and Adults of Yesso Scallop |
| title_full | Molecular Characterization and Expression Pattern of Paramyosin in Larvae and Adults of Yesso Scallop |
| title_fullStr | Molecular Characterization and Expression Pattern of Paramyosin in Larvae and Adults of Yesso Scallop |
| title_full_unstemmed | Molecular Characterization and Expression Pattern of Paramyosin in Larvae and Adults of Yesso Scallop |
| title_short | Molecular Characterization and Expression Pattern of Paramyosin in Larvae and Adults of Yesso Scallop |
| title_sort | molecular characterization and expression pattern of paramyosin in larvae and adults of yesso scallop |
| topic | scallops smooth adductor muscle paramyosin gene expression whole-mount in situ hybridization |
| url | https://www.mdpi.com/2079-7737/11/3/453 |
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