Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt
Excessive inorganic ions in vivo may lead to electrolyte disorders and induce damage to the human body. Therefore, preparation of enhanced bioactivity compounds, composed of activated organic cations and organic anions, is of great interest among researchers. In this work, glucosamine-heparin salt (...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/20/10/646 |
| _version_ | 1797440623173697536 |
|---|---|
| author | Qin Miao Qing Li Wenqiang Tan Yingqi Mi Bing Ma Jingjing Zhang Zhanyong Guo |
| author_facet | Qin Miao Qing Li Wenqiang Tan Yingqi Mi Bing Ma Jingjing Zhang Zhanyong Guo |
| author_sort | Qin Miao |
| collection | DOAJ |
| description | Excessive inorganic ions in vivo may lead to electrolyte disorders and induce damage to the human body. Therefore, preparation of enhanced bioactivity compounds, composed of activated organic cations and organic anions, is of great interest among researchers. In this work, glucosamine-heparin salt (GHS) was primarily synthesized with positively charged glucosamine hydrochloride (GAH) and negatively charged heparin sodium (Heps) by ion exchange method. Then, the detailed structural information of the GHS was characterized by FTIR, <sup>1</sup>H NMR spectroscopy and ICP-MS. In addition, its anticoagulant potency and antioxidant properties were evaluated, respectively. The results demonstrated that GHS salt achieved enhanced antioxidant activities, including 98.78% of O<sub>2</sub>•<sup>−</sup> radical scavenging activity, 91.23% of •OH radical scavenging rate and 66.49% of DPPH radical scavenging capacity at 1.6 mg/mL, severally. Meanwhile, anticoagulant potency (ATTP) of GHS strengthened from 153.10 ± 17.14 to 180.03 ± 6.02 at 0.75 μmol/L. Thus, introducing cationic glucosamine residues into GHS could improve its anticoagulant activity. The findings suggest that GHS product with a small amount of inorganic ions can greatly abate the prime cost of antioxidants and anticoagulants, and has significant economic benefits and practical significance. |
| first_indexed | 2024-03-09T12:11:57Z |
| format | Article |
| id | doaj.art-9a89328bc87e409ab14d9cc11d4c7e77 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T12:11:57Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-9a89328bc87e409ab14d9cc11d4c7e772023-11-30T22:51:35ZengMDPI AGMarine Drugs1660-33972022-10-01201064610.3390/md20100646Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin SaltQin Miao0Qing Li1Wenqiang Tan2Yingqi Mi3Bing Ma4Jingjing Zhang5Zhanyong Guo6Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaKey Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaKey Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaKey Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaKey Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaKey Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaKey Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaExcessive inorganic ions in vivo may lead to electrolyte disorders and induce damage to the human body. Therefore, preparation of enhanced bioactivity compounds, composed of activated organic cations and organic anions, is of great interest among researchers. In this work, glucosamine-heparin salt (GHS) was primarily synthesized with positively charged glucosamine hydrochloride (GAH) and negatively charged heparin sodium (Heps) by ion exchange method. Then, the detailed structural information of the GHS was characterized by FTIR, <sup>1</sup>H NMR spectroscopy and ICP-MS. In addition, its anticoagulant potency and antioxidant properties were evaluated, respectively. The results demonstrated that GHS salt achieved enhanced antioxidant activities, including 98.78% of O<sub>2</sub>•<sup>−</sup> radical scavenging activity, 91.23% of •OH radical scavenging rate and 66.49% of DPPH radical scavenging capacity at 1.6 mg/mL, severally. Meanwhile, anticoagulant potency (ATTP) of GHS strengthened from 153.10 ± 17.14 to 180.03 ± 6.02 at 0.75 μmol/L. Thus, introducing cationic glucosamine residues into GHS could improve its anticoagulant activity. The findings suggest that GHS product with a small amount of inorganic ions can greatly abate the prime cost of antioxidants and anticoagulants, and has significant economic benefits and practical significance.https://www.mdpi.com/1660-3397/20/10/646glucosamine-heparin saltglucosamine hydrochlorideheparin sodiumantioxidant activityanticoagulant activity |
| spellingShingle | Qin Miao Qing Li Wenqiang Tan Yingqi Mi Bing Ma Jingjing Zhang Zhanyong Guo Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt Marine Drugs glucosamine-heparin salt glucosamine hydrochloride heparin sodium antioxidant activity anticoagulant activity |
| title | Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt |
| title_full | Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt |
| title_fullStr | Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt |
| title_full_unstemmed | Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt |
| title_short | Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt |
| title_sort | preparation anticoagulant and antioxidant properties of glucosamine heparin salt |
| topic | glucosamine-heparin salt glucosamine hydrochloride heparin sodium antioxidant activity anticoagulant activity |
| url | https://www.mdpi.com/1660-3397/20/10/646 |
| work_keys_str_mv | AT qinmiao preparationanticoagulantandantioxidantpropertiesofglucosamineheparinsalt AT qingli preparationanticoagulantandantioxidantpropertiesofglucosamineheparinsalt AT wenqiangtan preparationanticoagulantandantioxidantpropertiesofglucosamineheparinsalt AT yingqimi preparationanticoagulantandantioxidantpropertiesofglucosamineheparinsalt AT bingma preparationanticoagulantandantioxidantpropertiesofglucosamineheparinsalt AT jingjingzhang preparationanticoagulantandantioxidantpropertiesofglucosamineheparinsalt AT zhanyongguo preparationanticoagulantandantioxidantpropertiesofglucosamineheparinsalt |