Profiling of Transcriptome-Wide N6-Methyladenosine (m6A) Modifications and Identifying m6A Associated Regulation in Sperm Tail Formation in <i>Anopheles sinensis</i>
Recent discoveries of reversible N6-methyladenosine (m6A) methylation on messenger RNA (mRNA) and mapping of m6A methylomes in many species have revealed potential regulatory functions of this RNA modification by m6A players—writers, readers, and erasers. Here, we first profile transcriptome-wide m6...
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/9/4630 |
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| author | Congshan Liu Jianping Cao Haobing Zhang Jiatong Wu Jianhai Yin |
| author_facet | Congshan Liu Jianping Cao Haobing Zhang Jiatong Wu Jianhai Yin |
| author_sort | Congshan Liu |
| collection | DOAJ |
| description | Recent discoveries of reversible N6-methyladenosine (m6A) methylation on messenger RNA (mRNA) and mapping of m6A methylomes in many species have revealed potential regulatory functions of this RNA modification by m6A players—writers, readers, and erasers. Here, we first profile transcriptome-wide m6A in female and male <i>Anopheles sinensis</i> and reveal that m6A is also a highly conserved modification of mRNA in mosquitoes. Distinct from mammals and yeast but similar to <i>Arabidopsis thaliana</i>, m6A in <i>An. sinensis</i> is enriched not only around the stop codon and within 3′-untranslated regions but also around the start codon and 5′-UTR. Gene ontology analysis indicates the unique distribution pattern of m6A in <i>An. sinensis</i> is associated with mosquito sex-specific pathways such as tRNA wobble uridine modification and phospholipid-binding in females, and peptidoglycan catabolic process, exosome and signal recognition particle, endoplasmic reticulum targeting, and RNA helicase activity in males. The positive correlation between m6A deposition and mRNA abundance indicates that m6A can play a role in regulating gene expression in mosquitoes. Furthermore, many spermatogenesis-associated genes, especially those related to mature sperm flagellum formation, are positively modulated by m6A methylation. A transcriptional regulatory network of m6A in <i>An. sinensis</i> is first profiled in the present study, especially in spermatogenesis, which may provide a new clue for the control of this disease-transmitting vector. |
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| spelling | doaj.art-9a89ca8e941c4ea6a04b5d0959e39a612023-11-23T08:19:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01239463010.3390/ijms23094630Profiling of Transcriptome-Wide N6-Methyladenosine (m6A) Modifications and Identifying m6A Associated Regulation in Sperm Tail Formation in <i>Anopheles sinensis</i>Congshan Liu0Jianping Cao1Haobing Zhang2Jiatong Wu3Jianhai Yin4National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, ChinaNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, ChinaNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, ChinaNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, ChinaNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, ChinaRecent discoveries of reversible N6-methyladenosine (m6A) methylation on messenger RNA (mRNA) and mapping of m6A methylomes in many species have revealed potential regulatory functions of this RNA modification by m6A players—writers, readers, and erasers. Here, we first profile transcriptome-wide m6A in female and male <i>Anopheles sinensis</i> and reveal that m6A is also a highly conserved modification of mRNA in mosquitoes. Distinct from mammals and yeast but similar to <i>Arabidopsis thaliana</i>, m6A in <i>An. sinensis</i> is enriched not only around the stop codon and within 3′-untranslated regions but also around the start codon and 5′-UTR. Gene ontology analysis indicates the unique distribution pattern of m6A in <i>An. sinensis</i> is associated with mosquito sex-specific pathways such as tRNA wobble uridine modification and phospholipid-binding in females, and peptidoglycan catabolic process, exosome and signal recognition particle, endoplasmic reticulum targeting, and RNA helicase activity in males. The positive correlation between m6A deposition and mRNA abundance indicates that m6A can play a role in regulating gene expression in mosquitoes. Furthermore, many spermatogenesis-associated genes, especially those related to mature sperm flagellum formation, are positively modulated by m6A methylation. A transcriptional regulatory network of m6A in <i>An. sinensis</i> is first profiled in the present study, especially in spermatogenesis, which may provide a new clue for the control of this disease-transmitting vector.https://www.mdpi.com/1422-0067/23/9/4630<i>Anopheles sinensis</i>epigeneticsm6Asex-specificspermatogenesissperm tail |
| spellingShingle | Congshan Liu Jianping Cao Haobing Zhang Jiatong Wu Jianhai Yin Profiling of Transcriptome-Wide N6-Methyladenosine (m6A) Modifications and Identifying m6A Associated Regulation in Sperm Tail Formation in <i>Anopheles sinensis</i> International Journal of Molecular Sciences <i>Anopheles sinensis</i> epigenetics m6A sex-specific spermatogenesis sperm tail |
| title | Profiling of Transcriptome-Wide N6-Methyladenosine (m6A) Modifications and Identifying m6A Associated Regulation in Sperm Tail Formation in <i>Anopheles sinensis</i> |
| title_full | Profiling of Transcriptome-Wide N6-Methyladenosine (m6A) Modifications and Identifying m6A Associated Regulation in Sperm Tail Formation in <i>Anopheles sinensis</i> |
| title_fullStr | Profiling of Transcriptome-Wide N6-Methyladenosine (m6A) Modifications and Identifying m6A Associated Regulation in Sperm Tail Formation in <i>Anopheles sinensis</i> |
| title_full_unstemmed | Profiling of Transcriptome-Wide N6-Methyladenosine (m6A) Modifications and Identifying m6A Associated Regulation in Sperm Tail Formation in <i>Anopheles sinensis</i> |
| title_short | Profiling of Transcriptome-Wide N6-Methyladenosine (m6A) Modifications and Identifying m6A Associated Regulation in Sperm Tail Formation in <i>Anopheles sinensis</i> |
| title_sort | profiling of transcriptome wide n6 methyladenosine m6a modifications and identifying m6a associated regulation in sperm tail formation in i anopheles sinensis i |
| topic | <i>Anopheles sinensis</i> epigenetics m6A sex-specific spermatogenesis sperm tail |
| url | https://www.mdpi.com/1422-0067/23/9/4630 |
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