Prevalence of quinolone-resistant uropathogenic Escherichia coli in a tertiary care hospital in south Iran
Yalda Malekzadegan,1 Elham Rastegar,1 Melika Moradi,1 Hamid Heidari,2 Hadi Sedigh Ebrahim-Saraie31Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; 2Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-06-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/prevalence-of-quinolone-resistant-uropathogenic-escherichia-coli-in-a--peer-reviewed-article-IDR |
| _version_ | 1819116667968421888 |
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| author | Malekzadegan Y Rastegar E Moradi M Heidari H Sedigh Ebrahim-Saraie H |
| author_facet | Malekzadegan Y Rastegar E Moradi M Heidari H Sedigh Ebrahim-Saraie H |
| author_sort | Malekzadegan Y |
| collection | DOAJ |
| description | Yalda Malekzadegan,1 Elham Rastegar,1 Melika Moradi,1 Hamid Heidari,2 Hadi Sedigh Ebrahim-Saraie31Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; 2Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Razi Clinical Research Development Center, Guilan University of Medical Sciences, Rasht, IranBackground: Quinolones are a family of synthetic antimicrobial agents with a broad antibacterial activity commonly used as a suitable therapy in patients with urinary tract infection (UTI). In the present study, we aimed to evaluate the prevalence of quinolones resistance and the presence of plasmid-mediated quinolone resistance (PMQR) genes among Escherichia coli isolates.Methods: This study was performed on a collection of 121 E. coli isolates derived from patients with UTI. Antimicrobial susceptibility to nalidixic acid, ciprofloxacin, levofloxacin, norfloxacin, and ofloxacin was specified by the disk diffusion method. The presence of PMQR genes was determined by PCR method.Results: Antibiotic susceptibility results showed that the highest and lowest resistance rates were against nalidixic acid (71.9%) and norfloxacin (44.6%), respectively. The molecular results showed that 40 (33.1%) and 15 (12.4%) of the isolates were positive for qnrS and qnrB genes, respectively. Meanwhile, 5 (4.1%) of the isolates were found positive for both genes, while none were found to be positive for qnrA gene. There was no significant association between the presence of qnr genes and higher antibiotic resistance.Conclusion: We found high levels of quinolones resistance (more than 40%) among E. coli strains isolated from patients with UTIs in the south of Iran. We further report the prevalence of PMQR genes among uropathogenic E. coli; however, it seems that these genes are not the main components of quinolone resistance in our region.Keywords: urinary tract infection, Escherichia coli, quinolone, antibiotic resistance |
| first_indexed | 2024-12-22T05:20:44Z |
| format | Article |
| id | doaj.art-9a8bfdcbcd4c4bd6a7f85a7dacb07cbd |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-22T05:20:44Z |
| publishDate | 2019-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-9a8bfdcbcd4c4bd6a7f85a7dacb07cbd2022-12-21T18:37:44ZengDove Medical PressInfection and Drug Resistance1178-69732019-06-01Volume 121683168946526Prevalence of quinolone-resistant uropathogenic Escherichia coli in a tertiary care hospital in south IranMalekzadegan YRastegar EMoradi MHeidari HSedigh Ebrahim-Saraie HYalda Malekzadegan,1 Elham Rastegar,1 Melika Moradi,1 Hamid Heidari,2 Hadi Sedigh Ebrahim-Saraie31Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; 2Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Razi Clinical Research Development Center, Guilan University of Medical Sciences, Rasht, IranBackground: Quinolones are a family of synthetic antimicrobial agents with a broad antibacterial activity commonly used as a suitable therapy in patients with urinary tract infection (UTI). In the present study, we aimed to evaluate the prevalence of quinolones resistance and the presence of plasmid-mediated quinolone resistance (PMQR) genes among Escherichia coli isolates.Methods: This study was performed on a collection of 121 E. coli isolates derived from patients with UTI. Antimicrobial susceptibility to nalidixic acid, ciprofloxacin, levofloxacin, norfloxacin, and ofloxacin was specified by the disk diffusion method. The presence of PMQR genes was determined by PCR method.Results: Antibiotic susceptibility results showed that the highest and lowest resistance rates were against nalidixic acid (71.9%) and norfloxacin (44.6%), respectively. The molecular results showed that 40 (33.1%) and 15 (12.4%) of the isolates were positive for qnrS and qnrB genes, respectively. Meanwhile, 5 (4.1%) of the isolates were found positive for both genes, while none were found to be positive for qnrA gene. There was no significant association between the presence of qnr genes and higher antibiotic resistance.Conclusion: We found high levels of quinolones resistance (more than 40%) among E. coli strains isolated from patients with UTIs in the south of Iran. We further report the prevalence of PMQR genes among uropathogenic E. coli; however, it seems that these genes are not the main components of quinolone resistance in our region.Keywords: urinary tract infection, Escherichia coli, quinolone, antibiotic resistancehttps://www.dovepress.com/prevalence-of-quinolone-resistant-uropathogenic-escherichia-coli-in-a--peer-reviewed-article-IDRUrinary tract infectionEscherichia coliQuinoloneAntibiotic resistance |
| spellingShingle | Malekzadegan Y Rastegar E Moradi M Heidari H Sedigh Ebrahim-Saraie H Prevalence of quinolone-resistant uropathogenic Escherichia coli in a tertiary care hospital in south Iran Infection and Drug Resistance Urinary tract infection Escherichia coli Quinolone Antibiotic resistance |
| title | Prevalence of quinolone-resistant uropathogenic Escherichia coli in a tertiary care hospital in south Iran |
| title_full | Prevalence of quinolone-resistant uropathogenic Escherichia coli in a tertiary care hospital in south Iran |
| title_fullStr | Prevalence of quinolone-resistant uropathogenic Escherichia coli in a tertiary care hospital in south Iran |
| title_full_unstemmed | Prevalence of quinolone-resistant uropathogenic Escherichia coli in a tertiary care hospital in south Iran |
| title_short | Prevalence of quinolone-resistant uropathogenic Escherichia coli in a tertiary care hospital in south Iran |
| title_sort | prevalence of quinolone resistant uropathogenic escherichia coli in a tertiary care hospital in south iran |
| topic | Urinary tract infection Escherichia coli Quinolone Antibiotic resistance |
| url | https://www.dovepress.com/prevalence-of-quinolone-resistant-uropathogenic-escherichia-coli-in-a--peer-reviewed-article-IDR |
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