Surface Markers Expression in the Mesenchymal Stem Cells Derived from Bone Marrow
Background and aim: Mesenchymal stem cells were separated from bone marrow by Friedenstein et al. for the first time. In addition to their cohesion, mesenchymal properties of the cells were proven by surface markers. The aim of this study was to isolate mesenchymal stem cells from mouse bone marrow...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Yasuj University Of Medical Sciences
2013-11-01
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| Series: | Armaghane Danesh Bimonthly Journal |
| Subjects: | |
| Online Access: | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-80&slc_lang=en&sid=1 |
| _version_ | 1811199989218541568 |
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| author | KH Bahrebar H Mohseni Kouchesfahan H Delavize M Nabiuni Z Nazari P Havasi |
| author_facet | KH Bahrebar H Mohseni Kouchesfahan H Delavize M Nabiuni Z Nazari P Havasi |
| author_sort | KH Bahrebar |
| collection | DOAJ |
| description | Background and aim: Mesenchymal stem cells were separated from bone marrow by Friedenstein et al. for the first time. In addition to their cohesion, mesenchymal properties of the cells were proven by surface markers. The aim of this study was to isolate mesenchymal stem cells from mouse bone marrow cultures and some surface markers in the obtained cells were expressed.
Methods: In this study, bone marrow cells in the femur and tibia bones were isolated from NMRI mice. Bone marrow mesenchymal stem cells were isolated to the bottom of culture dish based on cohesion properties. In addition to their cohesion, mesenchymal properties of the cells were proven by CD73, CD44, CD90, CD31, CD45 and CD29 markers, after the fourth passage using flow cytometry.
Results: In the primary culture, a heterogenous population of flattened, spindle and multi-faceted cells were observed. Spindle-shaped cells increased in later passages, so that almost all of third passage cells were spindle-shaped. Flow cytometry analysis of surface markers revealed high level mesenchymal stem cells from the fourth passage markers CD44, CD73 and CD29, and, low level of CD31, CD45 and CD90 markers.
Conclusion: It can be concluded that MSCs derived from bone marrow mouse have some positive and negative surface markers with high and low expression, respectively. The expression rate of these markers proves the mesenchymal properties of cells.
Keyword: Bone Marrow, Mesenchymal Stem Cells, Surface Markers |
| first_indexed | 2024-04-12T01:57:17Z |
| format | Article |
| id | doaj.art-9a8c9b0df75343fe934082503cff0315 |
| institution | Directory Open Access Journal |
| issn | 1728-6506 1728-6514 |
| language | fas |
| last_indexed | 2024-04-12T01:57:17Z |
| publishDate | 2013-11-01 |
| publisher | Yasuj University Of Medical Sciences |
| record_format | Article |
| series | Armaghane Danesh Bimonthly Journal |
| spelling | doaj.art-9a8c9b0df75343fe934082503cff03152022-12-22T03:52:47ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65061728-65142013-11-01187520529Surface Markers Expression in the Mesenchymal Stem Cells Derived from Bone MarrowKH Bahrebar0H Mohseni Kouchesfahan1H Delavize2M Nabiuni3Z Nazari4P Havasi5 Background and aim: Mesenchymal stem cells were separated from bone marrow by Friedenstein et al. for the first time. In addition to their cohesion, mesenchymal properties of the cells were proven by surface markers. The aim of this study was to isolate mesenchymal stem cells from mouse bone marrow cultures and some surface markers in the obtained cells were expressed. Methods: In this study, bone marrow cells in the femur and tibia bones were isolated from NMRI mice. Bone marrow mesenchymal stem cells were isolated to the bottom of culture dish based on cohesion properties. In addition to their cohesion, mesenchymal properties of the cells were proven by CD73, CD44, CD90, CD31, CD45 and CD29 markers, after the fourth passage using flow cytometry. Results: In the primary culture, a heterogenous population of flattened, spindle and multi-faceted cells were observed. Spindle-shaped cells increased in later passages, so that almost all of third passage cells were spindle-shaped. Flow cytometry analysis of surface markers revealed high level mesenchymal stem cells from the fourth passage markers CD44, CD73 and CD29, and, low level of CD31, CD45 and CD90 markers. Conclusion: It can be concluded that MSCs derived from bone marrow mouse have some positive and negative surface markers with high and low expression, respectively. The expression rate of these markers proves the mesenchymal properties of cells. Keyword: Bone Marrow, Mesenchymal Stem Cells, Surface Markershttp://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-80&slc_lang=en&sid=1Bone Marrow Mesenchymal Stem Cells Surface Markers |
| spellingShingle | KH Bahrebar H Mohseni Kouchesfahan H Delavize M Nabiuni Z Nazari P Havasi Surface Markers Expression in the Mesenchymal Stem Cells Derived from Bone Marrow Armaghane Danesh Bimonthly Journal Bone Marrow Mesenchymal Stem Cells Surface Markers |
| title | Surface Markers Expression in the Mesenchymal Stem Cells Derived from Bone Marrow |
| title_full | Surface Markers Expression in the Mesenchymal Stem Cells Derived from Bone Marrow |
| title_fullStr | Surface Markers Expression in the Mesenchymal Stem Cells Derived from Bone Marrow |
| title_full_unstemmed | Surface Markers Expression in the Mesenchymal Stem Cells Derived from Bone Marrow |
| title_short | Surface Markers Expression in the Mesenchymal Stem Cells Derived from Bone Marrow |
| title_sort | surface markers expression in the mesenchymal stem cells derived from bone marrow |
| topic | Bone Marrow Mesenchymal Stem Cells Surface Markers |
| url | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-80&slc_lang=en&sid=1 |
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