Immunosuppressive Effects of Multipotent Mesenchymal Stromal Cells on Graft-Versus-Host Disease in Rats Following Allogeneic Bone Marrow Transplantation
Objective: Graft-versus-host disease (GVHD) is a major obstacle to successful allogeneic bone marrow transplantation (allo-BMT). While multipotent mesenchymal stromal cells (MSCs) demonstrate alloresponse in vitro and in vivo, they also have clinical applications toward prevention or treatment of G...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2013-09-01
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| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-02223 |
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| author | Oral Nevruz Ferit Avcu A. Uğur Ural Aysel Pekel Bahar Dirican Mükerrem Safalı Elvin Akdağ Murat Beyzadeoğlu Tayfun İde Ali Sengül |
| author_facet | Oral Nevruz Ferit Avcu A. Uğur Ural Aysel Pekel Bahar Dirican Mükerrem Safalı Elvin Akdağ Murat Beyzadeoğlu Tayfun İde Ali Sengül |
| author_sort | Oral Nevruz |
| collection | DOAJ |
| description | Objective: Graft-versus-host disease (GVHD) is a major obstacle to successful allogeneic bone marrow transplantation (allo-BMT). While multipotent mesenchymal stromal cells (MSCs) demonstrate alloresponse in vitro and in vivo, they also have clinical applications toward prevention or treatment of GVHD. The aim of this study was to investigate the ability of MSCs to prevent or treat GVHD in a rat BMT model.
Materials and Methods: The GVHD model was established by transplantation of Sprague Dawley rats' bone marrow and spleen cells into lethally irradiated (950 cGy) SDxWistar rat recipients. A total of 49 rats were randomly assigned to 4 study and 3 control groups administered different GVHD prophylactic regimens including MSCs. After transplantation, clinical GVHD scores and survival status were monitored.
Results: All irradiated and untreated control mice with GVHD died. MSCs inhibited lethal GVHD as efficiently as the standard GVHD prophylactic regimen. The gross and histopathological findings of GVHD and the ratio of CD4/CD8 expression decreased. The subgroup given MSCs displayed higher in vivo proportions of CD25+ T cells and plasma interleukin-2 levels as compared to conventional GVHD treatment after allo-BMT.
Conclusion: Our results suggest that clinical use of MSCs in both prophylaxis against and treatment of established GVHD is effective. This study supports the use of MSCs in the prophylaxis and treatment of GVHD after allo-BMT; however, large scale studies are needed. |
| first_indexed | 2024-04-10T14:22:05Z |
| format | Article |
| id | doaj.art-9a8ed90f873140c5bd59808abdf7c135 |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T14:22:05Z |
| publishDate | 2013-09-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-9a8ed90f873140c5bd59808abdf7c1352023-02-15T16:09:16ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632013-09-0130325626210.4274/Tjh.2013.0032TJH-02223Immunosuppressive Effects of Multipotent Mesenchymal Stromal Cells on Graft-Versus-Host Disease in Rats Following Allogeneic Bone Marrow TransplantationOral Nevruz0Ferit Avcu1A. Uğur Ural2Aysel Pekel3Bahar Dirican4Mükerrem Safalı5Elvin Akdağ6Murat Beyzadeoğlu7Tayfun İde8Ali Sengül9Gülhane Medical Academy, Department Of Hematology, Ankara, TurkeyGülhane Medical Academy, Department Of Hematology, Ankara, TurkeyGülhane Medical Academy, Department Of Hematology, Ankara, TurkeyGülhane Medical Academy, Department Of Immunology, Ankara, TurkeyGülhane Medical Academy, Department Of Radiation Oncology, Ankara, TurkeyGülhane Medical Academy, Department Of Pathology, Ankara, TurkeyGülhane Medical Academy, Department Of Medical Oncology Research Center, Ankara, TurkeyGülhane Medical Academy, Department Of Radiation Oncology, Ankara, TurkeyGülhane Medical Academy, Department Of Medical Oncology Research Center, Ankara, TurkeyGülhane Medical Academy, Department Of Immunology, Ankara, TurkeyObjective: Graft-versus-host disease (GVHD) is a major obstacle to successful allogeneic bone marrow transplantation (allo-BMT). While multipotent mesenchymal stromal cells (MSCs) demonstrate alloresponse in vitro and in vivo, they also have clinical applications toward prevention or treatment of GVHD. The aim of this study was to investigate the ability of MSCs to prevent or treat GVHD in a rat BMT model. Materials and Methods: The GVHD model was established by transplantation of Sprague Dawley rats' bone marrow and spleen cells into lethally irradiated (950 cGy) SDxWistar rat recipients. A total of 49 rats were randomly assigned to 4 study and 3 control groups administered different GVHD prophylactic regimens including MSCs. After transplantation, clinical GVHD scores and survival status were monitored. Results: All irradiated and untreated control mice with GVHD died. MSCs inhibited lethal GVHD as efficiently as the standard GVHD prophylactic regimen. The gross and histopathological findings of GVHD and the ratio of CD4/CD8 expression decreased. The subgroup given MSCs displayed higher in vivo proportions of CD25+ T cells and plasma interleukin-2 levels as compared to conventional GVHD treatment after allo-BMT. Conclusion: Our results suggest that clinical use of MSCs in both prophylaxis against and treatment of established GVHD is effective. This study supports the use of MSCs in the prophylaxis and treatment of GVHD after allo-BMT; however, large scale studies are needed.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-02223mezenchimal stromal cellbone marrow transplantationi̇mmunsupresion |
| spellingShingle | Oral Nevruz Ferit Avcu A. Uğur Ural Aysel Pekel Bahar Dirican Mükerrem Safalı Elvin Akdağ Murat Beyzadeoğlu Tayfun İde Ali Sengül Immunosuppressive Effects of Multipotent Mesenchymal Stromal Cells on Graft-Versus-Host Disease in Rats Following Allogeneic Bone Marrow Transplantation Turkish Journal of Hematology mezenchimal stromal cell bone marrow transplantation i̇mmunsupresion |
| title | Immunosuppressive Effects of Multipotent Mesenchymal Stromal Cells on Graft-Versus-Host Disease in Rats Following Allogeneic Bone Marrow Transplantation |
| title_full | Immunosuppressive Effects of Multipotent Mesenchymal Stromal Cells on Graft-Versus-Host Disease in Rats Following Allogeneic Bone Marrow Transplantation |
| title_fullStr | Immunosuppressive Effects of Multipotent Mesenchymal Stromal Cells on Graft-Versus-Host Disease in Rats Following Allogeneic Bone Marrow Transplantation |
| title_full_unstemmed | Immunosuppressive Effects of Multipotent Mesenchymal Stromal Cells on Graft-Versus-Host Disease in Rats Following Allogeneic Bone Marrow Transplantation |
| title_short | Immunosuppressive Effects of Multipotent Mesenchymal Stromal Cells on Graft-Versus-Host Disease in Rats Following Allogeneic Bone Marrow Transplantation |
| title_sort | immunosuppressive effects of multipotent mesenchymal stromal cells on graft versus host disease in rats following allogeneic bone marrow transplantation |
| topic | mezenchimal stromal cell bone marrow transplantation i̇mmunsupresion |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-02223 |
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