Acute Effects of the Consumption of <i>Passiflora setacea</i> Juice on Metabolic Risk Factors and Gene Expression Profile in Humans
Background: <i>Passiflora setacea</i> (PS) is a passionfruit variety of the Brazilian savannah and is a rich source of plant food bioactives with potential anti-inflammatory activity. This study aimed to investigate the effect of an acute intake of PS juice upon inflammation, metabolic p...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/4/1104 |
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| author | Isabella de Araújo Esteves Duarte Dragan Milenkovic Tatiana Karla dos Santos Borges Artur Jordão de Magalhães Rosa Christine Morand Livia de Lacerda de Oliveira Ana Maria Costa |
| author_facet | Isabella de Araújo Esteves Duarte Dragan Milenkovic Tatiana Karla dos Santos Borges Artur Jordão de Magalhães Rosa Christine Morand Livia de Lacerda de Oliveira Ana Maria Costa |
| author_sort | Isabella de Araújo Esteves Duarte |
| collection | DOAJ |
| description | Background: <i>Passiflora setacea</i> (PS) is a passionfruit variety of the Brazilian savannah and is a rich source of plant food bioactives with potential anti-inflammatory activity. This study aimed to investigate the effect of an acute intake of PS juice upon inflammation, metabolic parameters, and gene expression on circulating immune cells in humans. Methods: Overweight male volunteers (<i>n</i> = 12) were enrolled in two double-blind placebo-controlled studies. Blood samples were collected from fasting volunteers 3 h after the consumption of 250 mL of PS juice or placebo (PB). Metabolic parameters (insulin, glucose, total cholesterol, high-density lipoprotein (LDL), high-density lipoprotein (HDL), and total triglycerides) and circulating cytokines were evaluated (study 1). Peripheral blood mononuclear cell (PBMC) from the same subjects were isolated and RNA was extracted for transcriptomic analyses using microarrays (study 2). Results: Insulin and homeostatic model assessment for insulin resistance (HOMA-IR) levels decreased statistically after the PS juice intake, whereas HDL level increased significantly. Interleukin (IL)-17A level increased after placebo consumption, whereas its level remained unchanged after PS juice consumption. Nutrigenomic analyses revealed 1327 differentially expressed genes after PS consumption, with modulated genes involved in processes such as inflammation, cell adhesion, or cytokine–cytokine receptor. Conclusion: Taken together, these clinical results support the hypothesis that PS consumption may help the prevention of cardiometabolic diseases. |
| first_indexed | 2024-03-10T20:26:19Z |
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| id | doaj.art-9a904d38bb76456aac36d893f6ee8708 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T20:26:19Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-9a904d38bb76456aac36d893f6ee87082023-11-19T21:45:50ZengMDPI AGNutrients2072-66432020-04-01124110410.3390/nu12041104Acute Effects of the Consumption of <i>Passiflora setacea</i> Juice on Metabolic Risk Factors and Gene Expression Profile in HumansIsabella de Araújo Esteves Duarte0Dragan Milenkovic1Tatiana Karla dos Santos Borges2Artur Jordão de Magalhães Rosa3Christine Morand4Livia de Lacerda de Oliveira5Ana Maria Costa6Postgraduate Program in Human Nutrition, College of Health Sciences, Campus Universitário Darcy Ribeiro, Universidade de Brasília, Brasília DF 70.910-900, BrazilUnité de Nutrition Humaine, Université Clermont Auvergne, INRAE, UNH, F-63000 Clermont-Ferrand, FranceLaboratory of Cellular Immunology, Faculty of Medicine, University of Brasilia, Brasilia DF 70.910-900, BrazilLaboratory of Food Science, Embrapa Cerrados, Planaltina DF 73.310-970, BrazilUnité de Nutrition Humaine, Université Clermont Auvergne, INRAE, UNH, F-63000 Clermont-Ferrand, FrancePostgraduate Program in Human Nutrition, College of Health Sciences, Campus Universitário Darcy Ribeiro, Universidade de Brasília, Brasília DF 70.910-900, BrazilLaboratory of Food Science, Embrapa Cerrados, Planaltina DF 73.310-970, BrazilBackground: <i>Passiflora setacea</i> (PS) is a passionfruit variety of the Brazilian savannah and is a rich source of plant food bioactives with potential anti-inflammatory activity. This study aimed to investigate the effect of an acute intake of PS juice upon inflammation, metabolic parameters, and gene expression on circulating immune cells in humans. Methods: Overweight male volunteers (<i>n</i> = 12) were enrolled in two double-blind placebo-controlled studies. Blood samples were collected from fasting volunteers 3 h after the consumption of 250 mL of PS juice or placebo (PB). Metabolic parameters (insulin, glucose, total cholesterol, high-density lipoprotein (LDL), high-density lipoprotein (HDL), and total triglycerides) and circulating cytokines were evaluated (study 1). Peripheral blood mononuclear cell (PBMC) from the same subjects were isolated and RNA was extracted for transcriptomic analyses using microarrays (study 2). Results: Insulin and homeostatic model assessment for insulin resistance (HOMA-IR) levels decreased statistically after the PS juice intake, whereas HDL level increased significantly. Interleukin (IL)-17A level increased after placebo consumption, whereas its level remained unchanged after PS juice consumption. Nutrigenomic analyses revealed 1327 differentially expressed genes after PS consumption, with modulated genes involved in processes such as inflammation, cell adhesion, or cytokine–cytokine receptor. Conclusion: Taken together, these clinical results support the hypothesis that PS consumption may help the prevention of cardiometabolic diseases.https://www.mdpi.com/2072-6643/12/4/1104<i>Passiflora setacea</i>bioactive compoundsphenolic compoundscardiovascular diseasesnutrigenomicsgene expression |
| spellingShingle | Isabella de Araújo Esteves Duarte Dragan Milenkovic Tatiana Karla dos Santos Borges Artur Jordão de Magalhães Rosa Christine Morand Livia de Lacerda de Oliveira Ana Maria Costa Acute Effects of the Consumption of <i>Passiflora setacea</i> Juice on Metabolic Risk Factors and Gene Expression Profile in Humans Nutrients <i>Passiflora setacea</i> bioactive compounds phenolic compounds cardiovascular diseases nutrigenomics gene expression |
| title | Acute Effects of the Consumption of <i>Passiflora setacea</i> Juice on Metabolic Risk Factors and Gene Expression Profile in Humans |
| title_full | Acute Effects of the Consumption of <i>Passiflora setacea</i> Juice on Metabolic Risk Factors and Gene Expression Profile in Humans |
| title_fullStr | Acute Effects of the Consumption of <i>Passiflora setacea</i> Juice on Metabolic Risk Factors and Gene Expression Profile in Humans |
| title_full_unstemmed | Acute Effects of the Consumption of <i>Passiflora setacea</i> Juice on Metabolic Risk Factors and Gene Expression Profile in Humans |
| title_short | Acute Effects of the Consumption of <i>Passiflora setacea</i> Juice on Metabolic Risk Factors and Gene Expression Profile in Humans |
| title_sort | acute effects of the consumption of i passiflora setacea i juice on metabolic risk factors and gene expression profile in humans |
| topic | <i>Passiflora setacea</i> bioactive compounds phenolic compounds cardiovascular diseases nutrigenomics gene expression |
| url | https://www.mdpi.com/2072-6643/12/4/1104 |
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