Evaluation of a recombinant rhoptry protein 2 enzyme-linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancy
The aim of this study was to evaluate an enzyme-linked immunoassay with recombinant rhoptry protein 2 (ELISA-rROP2) for its ability to detectToxoplasma gondii ROP2-specific IgG in samples from pregnant women. The study included 236 samples that were divided into groups according to serological scree...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2015-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600732&lng=en&tlng=en |
| _version_ | 1827831825284726784 |
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| author | Jaqueline Dario Capobiango Sthefany Pagliari Aline Kuhn Sbruzzi Pasquali Beatriz Nino Fernanda Pinto Ferreira Thaís Cabral Monica Nely Norder Tschurtschenthaler Italmar Teodorico Navarro João Luis Garcia Regina Mitsuka-Breganó Edna Maria Vissoci Reiche |
| author_facet | Jaqueline Dario Capobiango Sthefany Pagliari Aline Kuhn Sbruzzi Pasquali Beatriz Nino Fernanda Pinto Ferreira Thaís Cabral Monica Nely Norder Tschurtschenthaler Italmar Teodorico Navarro João Luis Garcia Regina Mitsuka-Breganó Edna Maria Vissoci Reiche |
| author_sort | Jaqueline Dario Capobiango |
| collection | DOAJ |
| description | The aim of this study was to evaluate an enzyme-linked immunoassay with recombinant rhoptry protein 2 (ELISA-rROP2) for its ability to detectToxoplasma gondii ROP2-specific IgG in samples from pregnant women. The study included 236 samples that were divided into groups according to serological screening profiles for toxoplasmosis: unexposed (n = 65), probable acute infection (n = 48), possible acute infection (n = 58) and exposed to the parasite (n = 65). When an indirect immunofluorescence assay forT. gondii-specific IgG was considered as a reference test, the ELISA-rROP2 had a sensitivity of 61.8%, specificity of 62.8%, predictive positive value of 76.6% and predictive negative value of 45.4% (p = 0.0002). The ELISA-rROP2 reacted with 62.5% of the samples from pregnant women with probable acute infection and 40% of the samples from pregnant women with previous exposure (p = 0.0180). Seropositivity was observed in 50/57 (87.7%) pregnant women with possible infection. The results underscored that T. gondii rROP2 is recognised by specific IgG antibodies in both the acute and chronic phases of toxoplasmosis acquired during pregnancy. However, the sensitivity of the ELISA-rROP2 was higher in the pregnant women with probable and possible acute infections and IgM reactivity. |
| first_indexed | 2024-03-12T05:01:04Z |
| format | Article |
| id | doaj.art-9a91de72104e4d1299f7a4ffa37ff947 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:01:04Z |
| publishDate | 2015-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-9a91de72104e4d1299f7a4ffa37ff9472023-09-03T09:11:46ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602015-09-01110673273810.1590/0074-02760150069S0074-02762015000600732Evaluation of a recombinant rhoptry protein 2 enzyme-linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancyJaqueline Dario CapobiangoSthefany PagliariAline Kuhn Sbruzzi PasqualiBeatriz NinoFernanda Pinto FerreiraThaís Cabral MonicaNely Norder TschurtschenthalerItalmar Teodorico NavarroJoão Luis GarciaRegina Mitsuka-BreganóEdna Maria Vissoci ReicheThe aim of this study was to evaluate an enzyme-linked immunoassay with recombinant rhoptry protein 2 (ELISA-rROP2) for its ability to detectToxoplasma gondii ROP2-specific IgG in samples from pregnant women. The study included 236 samples that were divided into groups according to serological screening profiles for toxoplasmosis: unexposed (n = 65), probable acute infection (n = 48), possible acute infection (n = 58) and exposed to the parasite (n = 65). When an indirect immunofluorescence assay forT. gondii-specific IgG was considered as a reference test, the ELISA-rROP2 had a sensitivity of 61.8%, specificity of 62.8%, predictive positive value of 76.6% and predictive negative value of 45.4% (p = 0.0002). The ELISA-rROP2 reacted with 62.5% of the samples from pregnant women with probable acute infection and 40% of the samples from pregnant women with previous exposure (p = 0.0180). Seropositivity was observed in 50/57 (87.7%) pregnant women with possible infection. The results underscored that T. gondii rROP2 is recognised by specific IgG antibodies in both the acute and chronic phases of toxoplasmosis acquired during pregnancy. However, the sensitivity of the ELISA-rROP2 was higher in the pregnant women with probable and possible acute infections and IgM reactivity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600732&lng=en&tlng=entoxoplasmosispregnancyrhoptry protein 2recombinant proteinsToxoplasma gondii |
| spellingShingle | Jaqueline Dario Capobiango Sthefany Pagliari Aline Kuhn Sbruzzi Pasquali Beatriz Nino Fernanda Pinto Ferreira Thaís Cabral Monica Nely Norder Tschurtschenthaler Italmar Teodorico Navarro João Luis Garcia Regina Mitsuka-Breganó Edna Maria Vissoci Reiche Evaluation of a recombinant rhoptry protein 2 enzyme-linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancy Memorias do Instituto Oswaldo Cruz toxoplasmosis pregnancy rhoptry protein 2 recombinant proteins Toxoplasma gondii |
| title | Evaluation of a recombinant rhoptry protein 2 enzyme-linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancy |
| title_full | Evaluation of a recombinant rhoptry protein 2 enzyme-linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancy |
| title_fullStr | Evaluation of a recombinant rhoptry protein 2 enzyme-linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancy |
| title_full_unstemmed | Evaluation of a recombinant rhoptry protein 2 enzyme-linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancy |
| title_short | Evaluation of a recombinant rhoptry protein 2 enzyme-linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancy |
| title_sort | evaluation of a recombinant rhoptry protein 2 enzyme linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancy |
| topic | toxoplasmosis pregnancy rhoptry protein 2 recombinant proteins Toxoplasma gondii |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600732&lng=en&tlng=en |
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