Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9

Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9

The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement for the 5′-NGG-3′ protospacer-adjacent motif (PAM) of Cas9 from Streptococcus pyogenes (SpCas9) limits its targeting sequences. Here, we report that a Cas9 ortholog from Staphylococcus aureu...

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Bibliographic Details
Main Authors: Yan Feng, Cheng Chen, Yuxiang Han, Zelin Chen, Xiaochan Lu, Fang Liang, Song Li, Wei Qin, Shuo Lin
Format: Article
Language:English
Published: Oxford University Press 2016-08-01
Series:G3: Genes, Genomes, Genetics
Subjects:
CRISPR/Cas9
SaCas9
KKH SaCas9 variant
gene editing
zebrafish
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.116.031914

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