MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation
Abstract Background Oocyte maturation and preimplantation embryo development are controlled by array of genes that are post-transcriptionally regulated by microRNAs. With respect to this, previously, we identified altered expression of microRNA-130b (miR-130b) during oocyte maturation. Here, we aime...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-06-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13048-017-0336-1 |
| _version_ | 1797972594916327424 |
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| author | Pritam Bala Sinha Dawit Tesfaye Franca Rings Munir Hossien Michael Hoelker Eva Held Christaine Neuhoff Ernst Tholen Karl Schellander Dessie Salilew-Wondim |
| author_facet | Pritam Bala Sinha Dawit Tesfaye Franca Rings Munir Hossien Michael Hoelker Eva Held Christaine Neuhoff Ernst Tholen Karl Schellander Dessie Salilew-Wondim |
| author_sort | Pritam Bala Sinha |
| collection | DOAJ |
| description | Abstract Background Oocyte maturation and preimplantation embryo development are controlled by array of genes that are post-transcriptionally regulated by microRNAs. With respect to this, previously, we identified altered expression of microRNA-130b (miR-130b) during oocyte maturation. Here, we aimed to investigate the role of miR-130b in bovine granulosa and cumulus cell function, oocyte maturation and preimplantation embryo development using gain- and loss-of- function approach. Methods For this study, the granulosa cells, cumulus cells and the oocytes were collected from ovaries obtained from slaughterhouse. The genes targeted by miR-130b were identified using dual-luciferase reporter assay. The role of miR-130b in granulosa and cumulus cell function was investigated by increasing and inhibiting its expression in in vitro cultured cells using miR-130b precursor and inhibitor, respectively while the role of miR-130b on oocyte development, immature oocytes were microinjected with miR-130b precursor and inhibitor and the polar body extrusion, the proportion of oocytes reaching to metaphase II stage and the mitochondrial were determined in each oocyte group 22 h after microinjection. Moreover, to investigate the role of miR-130b during preimplantation embryo development, zygote stage embryos were microinjected with miR-130b precursor or inhibitor and the cleavage rate, morula and blastocyst formation was analyzed in embryos derived from each zygote group after in vitro culture. Results The luciferase assay showed that SMAD5 and MSK1 genes were identified as the direct targets of miR-130b. Overexpression of miR-130b increased the granulosa and cumulus cell proliferation, while inhibition showed the opposite phenotype. Apart from these, modulation of miR-130b altered the lactate production and cholesterol biosynthesis in cumulus cells. Furthermore, inhibition of miR-130b expression during oocyte in vitro maturation reduced the first polar body extrusion, the proportion of oocytes reaching to metaphase II stage and the mitochondrial activity, while inhibition of miR-130b during preimplantation embryo development significantly reduced morula and blastocyst formation. Conclusion This study demonstrated that in vitro functional modulation of miR-130b affected granulosa and cumulus cell proliferation and survival, oocyte maturation, morula and blastocyst formation suggesting that miR-130b is involved in bovine oocyte maturation and preimplantation embryo development. |
| first_indexed | 2024-04-11T03:50:57Z |
| format | Article |
| id | doaj.art-9a92acb1ec214a1485a91d5b34a896af |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T03:50:57Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-9a92acb1ec214a1485a91d5b34a896af2023-01-02T01:58:29ZengBMCJournal of Ovarian Research1757-22152017-06-0110111810.1186/s13048-017-0336-1MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formationPritam Bala Sinha0Dawit Tesfaye1Franca Rings2Munir Hossien3Michael Hoelker4Eva Held5Christaine Neuhoff6Ernst Tholen7Karl Schellander8Dessie Salilew-Wondim9Present address: Department of Biotechnology, Engineering and Applied Sciences, Amity University RanchiInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of BonnTeaching and Research Station Frankenforst, Faculty of Agriculture, University of BonnPresent address: Department of Animal Breeding and Genetics, Bangladesh Agricultural UniversityInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of BonnInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of BonnInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of BonnInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of BonnInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of BonnInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of BonnAbstract Background Oocyte maturation and preimplantation embryo development are controlled by array of genes that are post-transcriptionally regulated by microRNAs. With respect to this, previously, we identified altered expression of microRNA-130b (miR-130b) during oocyte maturation. Here, we aimed to investigate the role of miR-130b in bovine granulosa and cumulus cell function, oocyte maturation and preimplantation embryo development using gain- and loss-of- function approach. Methods For this study, the granulosa cells, cumulus cells and the oocytes were collected from ovaries obtained from slaughterhouse. The genes targeted by miR-130b were identified using dual-luciferase reporter assay. The role of miR-130b in granulosa and cumulus cell function was investigated by increasing and inhibiting its expression in in vitro cultured cells using miR-130b precursor and inhibitor, respectively while the role of miR-130b on oocyte development, immature oocytes were microinjected with miR-130b precursor and inhibitor and the polar body extrusion, the proportion of oocytes reaching to metaphase II stage and the mitochondrial were determined in each oocyte group 22 h after microinjection. Moreover, to investigate the role of miR-130b during preimplantation embryo development, zygote stage embryos were microinjected with miR-130b precursor or inhibitor and the cleavage rate, morula and blastocyst formation was analyzed in embryos derived from each zygote group after in vitro culture. Results The luciferase assay showed that SMAD5 and MSK1 genes were identified as the direct targets of miR-130b. Overexpression of miR-130b increased the granulosa and cumulus cell proliferation, while inhibition showed the opposite phenotype. Apart from these, modulation of miR-130b altered the lactate production and cholesterol biosynthesis in cumulus cells. Furthermore, inhibition of miR-130b expression during oocyte in vitro maturation reduced the first polar body extrusion, the proportion of oocytes reaching to metaphase II stage and the mitochondrial activity, while inhibition of miR-130b during preimplantation embryo development significantly reduced morula and blastocyst formation. Conclusion This study demonstrated that in vitro functional modulation of miR-130b affected granulosa and cumulus cell proliferation and survival, oocyte maturation, morula and blastocyst formation suggesting that miR-130b is involved in bovine oocyte maturation and preimplantation embryo development.http://link.springer.com/article/10.1186/s13048-017-0336-1OocyteEmbryomiR-130bMitochondrial activity |
| spellingShingle | Pritam Bala Sinha Dawit Tesfaye Franca Rings Munir Hossien Michael Hoelker Eva Held Christaine Neuhoff Ernst Tholen Karl Schellander Dessie Salilew-Wondim MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation Journal of Ovarian Research Oocyte Embryo miR-130b Mitochondrial activity |
| title | MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation |
| title_full | MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation |
| title_fullStr | MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation |
| title_full_unstemmed | MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation |
| title_short | MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation |
| title_sort | microrna 130b is involved in bovine granulosa and cumulus cells function oocyte maturation and blastocyst formation |
| topic | Oocyte Embryo miR-130b Mitochondrial activity |
| url | http://link.springer.com/article/10.1186/s13048-017-0336-1 |
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