Ex vivo evaluation of antibiotic sensitivity in samples from endodontic infections
ABSTRACTObjective To develop an in vitro model for real-time monitoring of endodontic biofilm growth and evaluate the ex vivo effect of antibiotics on biofilm growth.Material and Methods Root canal samples were taken from 40 patients and inoculated into 96-well plates in a system that measures biofi...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2022.2160536 |
| _version_ | 1797449974735175680 |
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| author | Álvaro Villanueva-Castellote Carmen Llena Puy Miguel Carda-Diéguez Álex Mira María D. Ferrer |
| author_facet | Álvaro Villanueva-Castellote Carmen Llena Puy Miguel Carda-Diéguez Álex Mira María D. Ferrer |
| author_sort | Álvaro Villanueva-Castellote |
| collection | DOAJ |
| description | ABSTRACTObjective To develop an in vitro model for real-time monitoring of endodontic biofilm growth and evaluate the ex vivo effect of antibiotics on biofilm growth.Material and Methods Root canal samples were taken from 40 patients and inoculated into 96-well plates in a system that measures biofilm growth through electrical impedance. Biofilm bacterial composition at the genus and species level was analyzed by Illumina sequencing. ANCOM-BC corrected data were used to compare bacterial composition after antibiotic treatment through compositional analysis, and to compare microbiological with clinical data.Results The stationary phase was reached at 8 hours. The biofilm formed had a similar bacterial composition to the inoculum, and Enterococcus faecalis was virtually absent from the samples. The bacterial composition and the effect of antibiotics were sample-dependent. Metronidazole was the antibiotic that most inhibited biofilm formation and azithromycin the one that inhibited it in the highest percentage of cases. The antibiotic effect could not be related to the biofilm original bacterial composition.Conclusions The impedance system allowed real-time monitoring of endodontic biofilm formation, and we propose it as a model for ex vivo evaluation of the whole biofilm susceptibility to antimicrobials, as opposed to evaluating antibiotic sensitivity of specific bacterial isolates. |
| first_indexed | 2024-03-09T14:32:49Z |
| format | Article |
| id | doaj.art-9a92ec5246e7439db2314ca893bf9ea9 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-03-09T14:32:49Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-9a92ec5246e7439db2314ca893bf9ea92023-11-27T17:25:00ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972023-12-0115110.1080/20002297.2022.2160536Ex vivo evaluation of antibiotic sensitivity in samples from endodontic infectionsÁlvaro Villanueva-Castellote0Carmen Llena Puy1Miguel Carda-Diéguez2Álex Mira3María D. Ferrer4Department of Stomatology, Universitat de València, Valencia, SpainDepartment of Stomatology, Universitat de València, Valencia, SpainGenomics&Health Department, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO), Valencia, SpainGenomics&Health Department, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO), Valencia, SpainGenomics&Health Department, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO), Valencia, SpainABSTRACTObjective To develop an in vitro model for real-time monitoring of endodontic biofilm growth and evaluate the ex vivo effect of antibiotics on biofilm growth.Material and Methods Root canal samples were taken from 40 patients and inoculated into 96-well plates in a system that measures biofilm growth through electrical impedance. Biofilm bacterial composition at the genus and species level was analyzed by Illumina sequencing. ANCOM-BC corrected data were used to compare bacterial composition after antibiotic treatment through compositional analysis, and to compare microbiological with clinical data.Results The stationary phase was reached at 8 hours. The biofilm formed had a similar bacterial composition to the inoculum, and Enterococcus faecalis was virtually absent from the samples. The bacterial composition and the effect of antibiotics were sample-dependent. Metronidazole was the antibiotic that most inhibited biofilm formation and azithromycin the one that inhibited it in the highest percentage of cases. The antibiotic effect could not be related to the biofilm original bacterial composition.Conclusions The impedance system allowed real-time monitoring of endodontic biofilm formation, and we propose it as a model for ex vivo evaluation of the whole biofilm susceptibility to antimicrobials, as opposed to evaluating antibiotic sensitivity of specific bacterial isolates.https://www.tandfonline.com/doi/10.1080/20002297.2022.2160536Antibioticbiofilmbiofilm modelendodonticreal time cell analysis |
| spellingShingle | Álvaro Villanueva-Castellote Carmen Llena Puy Miguel Carda-Diéguez Álex Mira María D. Ferrer Ex vivo evaluation of antibiotic sensitivity in samples from endodontic infections Journal of Oral Microbiology Antibiotic biofilm biofilm model endodontic real time cell analysis |
| title | Ex vivo evaluation of antibiotic sensitivity in samples from endodontic infections |
| title_full | Ex vivo evaluation of antibiotic sensitivity in samples from endodontic infections |
| title_fullStr | Ex vivo evaluation of antibiotic sensitivity in samples from endodontic infections |
| title_full_unstemmed | Ex vivo evaluation of antibiotic sensitivity in samples from endodontic infections |
| title_short | Ex vivo evaluation of antibiotic sensitivity in samples from endodontic infections |
| title_sort | ex vivo evaluation of antibiotic sensitivity in samples from endodontic infections |
| topic | Antibiotic biofilm biofilm model endodontic real time cell analysis |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2022.2160536 |
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