Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathway
ObjectiveThe bacterial cellulose membrane (BCM) has been widely studied and applied as a new biomaterial for wound healing, but causes pain with frequent dressing changes. Local application of bone marrow mesenchymal stem cells (BMSCs) requires a niche. Furthermore, the effect and mechanism of the B...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Surgery |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fsurg.2022.1027067/full |
| _version_ | 1797952084253868032 |
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| author | Xiaoyang Wang Jie Zhao Xiaochuan Wang Jingjuan Zhang Yi Wang Xinyue Wang Shanshan Jia Nian Shi Meiqi Lu Hongxia Su Jixun Zhang Duyin Jiang Duyin Jiang |
| author_facet | Xiaoyang Wang Jie Zhao Xiaochuan Wang Jingjuan Zhang Yi Wang Xinyue Wang Shanshan Jia Nian Shi Meiqi Lu Hongxia Su Jixun Zhang Duyin Jiang Duyin Jiang |
| author_sort | Xiaoyang Wang |
| collection | DOAJ |
| description | ObjectiveThe bacterial cellulose membrane (BCM) has been widely studied and applied as a new biomaterial for wound healing, but causes pain with frequent dressing changes. Local application of bone marrow mesenchymal stem cells (BMSCs) requires a niche. Furthermore, the effect and mechanism of the BCM combined with BMSCs have not been reported.MethodsMorphological and chemical identifications of BCMs were investigated by porosity analyses, scanning electron microscopy, and Fourier-transform infrared spectroscopy. Biological wound dressings (BWDs) were prepared by the BCM in combination with BMSCs. The biological effects of BWDs on human dermal fibroblast (HDF) and VEGF-A in human vascular endothelial cells (HuVECs) were detected in vitro, and the effect of BWDs on acute wounds in mice was detected in vivo. Collagen and angiogenesis were evaluated through hematoxylin–eosin staining and Masson staining. The expressions of COL-1 and VEGF-A and the activation of the Notch signaling pathway in vivo and in vitro were detected by quantitative reverse-transcriptase polymerase chain reaction.ResultsThe BCM had a nanoscale structure and provided a partial niche for the survival and proliferation of BMSCs. BWDs were successfully prepared and regulated the biological behaviors of wound healing-related cells in vitro and upregulated the expressions of COL-1 in HDF and VEGF-A in HuVECs. BWDs promoted wound healing by increasing collagen type I synthesis and angiogenesis in acute wounds in mice.ConclusionsBWDs prepared by the combination of nanomaterial BCMs and BMSCs facilitated acute wound healing, which may be regulated by activating the Notch signaling pathway. |
| first_indexed | 2024-04-10T22:40:49Z |
| format | Article |
| id | doaj.art-9a967a62c1a543dba27496fe48b7b20f |
| institution | Directory Open Access Journal |
| issn | 2296-875X |
| language | English |
| last_indexed | 2024-04-10T22:40:49Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Surgery |
| spelling | doaj.art-9a967a62c1a543dba27496fe48b7b20f2023-01-16T05:23:29ZengFrontiers Media S.A.Frontiers in Surgery2296-875X2023-01-01910.3389/fsurg.2022.10270671027067Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathwayXiaoyang Wang0Jie Zhao1Xiaochuan Wang2Jingjuan Zhang3Yi Wang4Xinyue Wang5Shanshan Jia6Nian Shi7Meiqi Lu8Hongxia Su9Jixun Zhang10Duyin Jiang11Duyin Jiang12Department of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, ChinaEmergency Medicine Center, The Second Hospital of Shandong University, Jinan, ChinaDepartment of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, ChinaDepartment of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, ChinaDepartment of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, ChinaDepartment of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, ChinaDepartment of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, ChinaDepartment of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, ChinaDepartment of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, ChinaShandong Nameide Biotechnology Limited Company, Jinan, ChinaDepartment of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, ChinaDepartment of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, ChinaEmergency Medicine Center, The Second Hospital of Shandong University, Jinan, ChinaObjectiveThe bacterial cellulose membrane (BCM) has been widely studied and applied as a new biomaterial for wound healing, but causes pain with frequent dressing changes. Local application of bone marrow mesenchymal stem cells (BMSCs) requires a niche. Furthermore, the effect and mechanism of the BCM combined with BMSCs have not been reported.MethodsMorphological and chemical identifications of BCMs were investigated by porosity analyses, scanning electron microscopy, and Fourier-transform infrared spectroscopy. Biological wound dressings (BWDs) were prepared by the BCM in combination with BMSCs. The biological effects of BWDs on human dermal fibroblast (HDF) and VEGF-A in human vascular endothelial cells (HuVECs) were detected in vitro, and the effect of BWDs on acute wounds in mice was detected in vivo. Collagen and angiogenesis were evaluated through hematoxylin–eosin staining and Masson staining. The expressions of COL-1 and VEGF-A and the activation of the Notch signaling pathway in vivo and in vitro were detected by quantitative reverse-transcriptase polymerase chain reaction.ResultsThe BCM had a nanoscale structure and provided a partial niche for the survival and proliferation of BMSCs. BWDs were successfully prepared and regulated the biological behaviors of wound healing-related cells in vitro and upregulated the expressions of COL-1 in HDF and VEGF-A in HuVECs. BWDs promoted wound healing by increasing collagen type I synthesis and angiogenesis in acute wounds in mice.ConclusionsBWDs prepared by the combination of nanomaterial BCMs and BMSCs facilitated acute wound healing, which may be regulated by activating the Notch signaling pathway.https://www.frontiersin.org/articles/10.3389/fsurg.2022.1027067/fullbacterial celluloseBMSCsbioactive wound dressingnanomaterialnotch signaling pathwaytissue repair |
| spellingShingle | Xiaoyang Wang Jie Zhao Xiaochuan Wang Jingjuan Zhang Yi Wang Xinyue Wang Shanshan Jia Nian Shi Meiqi Lu Hongxia Su Jixun Zhang Duyin Jiang Duyin Jiang Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathway Frontiers in Surgery bacterial cellulose BMSCs bioactive wound dressing nanomaterial notch signaling pathway tissue repair |
| title | Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathway |
| title_full | Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathway |
| title_fullStr | Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathway |
| title_full_unstemmed | Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathway |
| title_short | Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathway |
| title_sort | bacterial cellulose membrane combined with bmscs promotes wound healing by activating the notch signaling pathway |
| topic | bacterial cellulose BMSCs bioactive wound dressing nanomaterial notch signaling pathway tissue repair |
| url | https://www.frontiersin.org/articles/10.3389/fsurg.2022.1027067/full |
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