Fluoropolymer Coated DNA Nanoclews for Volumetric Visualization of Oligonucleotides Delivery and Near Infrared Light Activated Anti‐Angiogenic Oncotherapy
Abstract The potential of microRNA regulation in oncotherapy is limited by the lack of delivery vehicles. Herein, it is shown that fluoropolymer coated DNA nanoclews (FNCs) provide outstanding ability to deliver oligonucleotide through circulation and realize near infrared (NIR) light activated angi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-11-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202304633 |
| _version_ | 1827762387730563072 |
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| author | Peng Zhang Ranran Guo Haiting Zhang Wuli Yang Ye Tian |
| author_facet | Peng Zhang Ranran Guo Haiting Zhang Wuli Yang Ye Tian |
| author_sort | Peng Zhang |
| collection | DOAJ |
| description | Abstract The potential of microRNA regulation in oncotherapy is limited by the lack of delivery vehicles. Herein, it is shown that fluoropolymer coated DNA nanoclews (FNCs) provide outstanding ability to deliver oligonucleotide through circulation and realize near infrared (NIR) light activated angiogenesis suppression to abrogate tumors. Oligonucleotides are loaded in DNA nanoclews through sequence specific bindings and then a fluorinated zwitterionic polymer is coated onto the surface of nanoclews. Further incorporating quantum dots in the polymer coating endows the vectors with NIR‐IIb (1500–1700 nm) fluorescence and NIR light triggered release ability. The FNC vector can deliver oligonucleotides to cancer cells systemically and realize on‐demand cytosolic release of the cargo with high transfection efficiency. Taking advantage of the NIR‐IIb emission, the whole delivery process of FNCs is visualized volumetrically in vivo with a NIR light sheet microscope. Loaded by FNCs, an oligonucleotide can effectively silence the target miRNA when activated with NIR light, and inhibit angiogenesis inside tumor, leading to complete ablation of cancer. These findings suggest FNCs can be used as an efficient oligonucleotide delivery platform to modulate the expression of endogenous microRNA in gene therapy of cancer. |
| first_indexed | 2024-03-11T10:28:47Z |
| format | Article |
| id | doaj.art-9a96bd3412094651b862989ebbccc67f |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-11T10:28:47Z |
| publishDate | 2023-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-9a96bd3412094651b862989ebbccc67f2023-11-15T05:46:36ZengWileyAdvanced Science2198-38442023-11-011032n/an/a10.1002/advs.202304633Fluoropolymer Coated DNA Nanoclews for Volumetric Visualization of Oligonucleotides Delivery and Near Infrared Light Activated Anti‐Angiogenic OncotherapyPeng Zhang0Ranran Guo1Haiting Zhang2Wuli Yang3Ye Tian4Biomaterials Research Center School of Biomedical Engineering Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University Guangzhou 510515 ChinaSchool of Biomedical Engineering Guangzhou Medical University Guangzhou 510182 ChinaBiomaterials Research Center School of Biomedical Engineering Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University Guangzhou 510515 ChinaState Key Laboratory of Molecular Engineering of Polymers & Department of Macromolecular Science Fudan University Shanghai 200438 ChinaBiomaterials Research Center School of Biomedical Engineering Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University Guangzhou 510515 ChinaAbstract The potential of microRNA regulation in oncotherapy is limited by the lack of delivery vehicles. Herein, it is shown that fluoropolymer coated DNA nanoclews (FNCs) provide outstanding ability to deliver oligonucleotide through circulation and realize near infrared (NIR) light activated angiogenesis suppression to abrogate tumors. Oligonucleotides are loaded in DNA nanoclews through sequence specific bindings and then a fluorinated zwitterionic polymer is coated onto the surface of nanoclews. Further incorporating quantum dots in the polymer coating endows the vectors with NIR‐IIb (1500–1700 nm) fluorescence and NIR light triggered release ability. The FNC vector can deliver oligonucleotides to cancer cells systemically and realize on‐demand cytosolic release of the cargo with high transfection efficiency. Taking advantage of the NIR‐IIb emission, the whole delivery process of FNCs is visualized volumetrically in vivo with a NIR light sheet microscope. Loaded by FNCs, an oligonucleotide can effectively silence the target miRNA when activated with NIR light, and inhibit angiogenesis inside tumor, leading to complete ablation of cancer. These findings suggest FNCs can be used as an efficient oligonucleotide delivery platform to modulate the expression of endogenous microRNA in gene therapy of cancer.https://doi.org/10.1002/advs.202304633angiogenesis suppressionDNA nanostructuresgene therapyoligonucleotides deliveryvolumetric imaging |
| spellingShingle | Peng Zhang Ranran Guo Haiting Zhang Wuli Yang Ye Tian Fluoropolymer Coated DNA Nanoclews for Volumetric Visualization of Oligonucleotides Delivery and Near Infrared Light Activated Anti‐Angiogenic Oncotherapy Advanced Science angiogenesis suppression DNA nanostructures gene therapy oligonucleotides delivery volumetric imaging |
| title | Fluoropolymer Coated DNA Nanoclews for Volumetric Visualization of Oligonucleotides Delivery and Near Infrared Light Activated Anti‐Angiogenic Oncotherapy |
| title_full | Fluoropolymer Coated DNA Nanoclews for Volumetric Visualization of Oligonucleotides Delivery and Near Infrared Light Activated Anti‐Angiogenic Oncotherapy |
| title_fullStr | Fluoropolymer Coated DNA Nanoclews for Volumetric Visualization of Oligonucleotides Delivery and Near Infrared Light Activated Anti‐Angiogenic Oncotherapy |
| title_full_unstemmed | Fluoropolymer Coated DNA Nanoclews for Volumetric Visualization of Oligonucleotides Delivery and Near Infrared Light Activated Anti‐Angiogenic Oncotherapy |
| title_short | Fluoropolymer Coated DNA Nanoclews for Volumetric Visualization of Oligonucleotides Delivery and Near Infrared Light Activated Anti‐Angiogenic Oncotherapy |
| title_sort | fluoropolymer coated dna nanoclews for volumetric visualization of oligonucleotides delivery and near infrared light activated anti angiogenic oncotherapy |
| topic | angiogenesis suppression DNA nanostructures gene therapy oligonucleotides delivery volumetric imaging |
| url | https://doi.org/10.1002/advs.202304633 |
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