Loss of PIM1 correlates with progression and prognosis of salivary adenoid cystic carcinoma (SACC)
Abstract Background Increasing evidence indicates that PIM1 is a potential prognostic marker and target for cancer treatment but its precise mechanisms of action remain to be determined in salivary adenoid cystic carcinoma (SACC). This study aims to decipher the prognostic and mechanistic role of PI...
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | Cancer Cell International |
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| Online Access: | http://link.springer.com/article/10.1186/s12935-018-0518-y |
| _version_ | 1811334273178796032 |
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| author | Jiajie Xu Xin Zhu Qingling Li Chao Chen Zhenying Guo Zhuo Tan Chuanming Zheng Minghua Ge |
| author_facet | Jiajie Xu Xin Zhu Qingling Li Chao Chen Zhenying Guo Zhuo Tan Chuanming Zheng Minghua Ge |
| author_sort | Jiajie Xu |
| collection | DOAJ |
| description | Abstract Background Increasing evidence indicates that PIM1 is a potential prognostic marker and target for cancer treatment but its precise mechanisms of action remain to be determined in salivary adenoid cystic carcinoma (SACC). This study aims to decipher the prognostic and mechanistic role of PIM1 in progression of SACC cells and tumor tissues. Methods A SACC cell line (ACC-M) was transfected with shRNA plasmids targeting the PIM1 gene. The expression levels of PIM1, RUNX3 and p21 were measured by quantitative real-time PCR and western blot. Subcellular translocalization of RUNX3 and p21 proteins was assessed using immunofluorescence, and cell cycle phase was quantified using flow cytometry. A total of 97 SACC patients were retrospectively analyzed by clinicopathologic characteristics and survival outcomes. Results After down-regulation of PIM1 in ACC-M cells, RUNX3 and p21 proteins were translocated from cytoplasm to nucleus, with a decrease of p21 expression and increase of G0/G1 phase cells. PIM1 and RUNX3 levels show a distinct covariance. PIM1 is associated with T-status, lymph node involvement, nerve invasion, and distant metastasis in SACC tissues. Patients with low PIM1 level had a better outcome than those with higher PIM1 level. Conclusions PIM1 is multifunctional in ACC-M cells and it serves as a neoteric therapeutic target and potential prognostic marker for SACC patients. |
| first_indexed | 2024-04-13T17:06:13Z |
| format | Article |
| id | doaj.art-9a977615ca4e48acbbd4e863d94c9fe4 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-04-13T17:06:13Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-9a977615ca4e48acbbd4e863d94c9fe42022-12-22T02:38:28ZengBMCCancer Cell International1475-28672018-02-0118111110.1186/s12935-018-0518-yLoss of PIM1 correlates with progression and prognosis of salivary adenoid cystic carcinoma (SACC)Jiajie Xu0Xin Zhu1Qingling Li2Chao Chen3Zhenying Guo4Zhuo Tan5Chuanming Zheng6Minghua Ge7Department of Head and Neck Surgery, Zhejiang Cancer HospitalZhejiang Cancer Research InstituteDepartment of Medical Oncology, Zhejiang Cancer HospitalDepartment of Head and Neck Surgery, Zhejiang Cancer HospitalDepartment of Pathology, Zhejiang Cancer HospitalDepartment of Head and Neck Surgery, Zhejiang Cancer HospitalDepartment of Head and Neck Surgery, Zhejiang Cancer HospitalDepartment of Head and Neck Surgery, Zhejiang Cancer HospitalAbstract Background Increasing evidence indicates that PIM1 is a potential prognostic marker and target for cancer treatment but its precise mechanisms of action remain to be determined in salivary adenoid cystic carcinoma (SACC). This study aims to decipher the prognostic and mechanistic role of PIM1 in progression of SACC cells and tumor tissues. Methods A SACC cell line (ACC-M) was transfected with shRNA plasmids targeting the PIM1 gene. The expression levels of PIM1, RUNX3 and p21 were measured by quantitative real-time PCR and western blot. Subcellular translocalization of RUNX3 and p21 proteins was assessed using immunofluorescence, and cell cycle phase was quantified using flow cytometry. A total of 97 SACC patients were retrospectively analyzed by clinicopathologic characteristics and survival outcomes. Results After down-regulation of PIM1 in ACC-M cells, RUNX3 and p21 proteins were translocated from cytoplasm to nucleus, with a decrease of p21 expression and increase of G0/G1 phase cells. PIM1 and RUNX3 levels show a distinct covariance. PIM1 is associated with T-status, lymph node involvement, nerve invasion, and distant metastasis in SACC tissues. Patients with low PIM1 level had a better outcome than those with higher PIM1 level. Conclusions PIM1 is multifunctional in ACC-M cells and it serves as a neoteric therapeutic target and potential prognostic marker for SACC patients.http://link.springer.com/article/10.1186/s12935-018-0518-ySalivary adenoid cystic carcinomaPIM1p21RUNX3Short hairpin RNA |
| spellingShingle | Jiajie Xu Xin Zhu Qingling Li Chao Chen Zhenying Guo Zhuo Tan Chuanming Zheng Minghua Ge Loss of PIM1 correlates with progression and prognosis of salivary adenoid cystic carcinoma (SACC) Cancer Cell International Salivary adenoid cystic carcinoma PIM1 p21 RUNX3 Short hairpin RNA |
| title | Loss of PIM1 correlates with progression and prognosis of salivary adenoid cystic carcinoma (SACC) |
| title_full | Loss of PIM1 correlates with progression and prognosis of salivary adenoid cystic carcinoma (SACC) |
| title_fullStr | Loss of PIM1 correlates with progression and prognosis of salivary adenoid cystic carcinoma (SACC) |
| title_full_unstemmed | Loss of PIM1 correlates with progression and prognosis of salivary adenoid cystic carcinoma (SACC) |
| title_short | Loss of PIM1 correlates with progression and prognosis of salivary adenoid cystic carcinoma (SACC) |
| title_sort | loss of pim1 correlates with progression and prognosis of salivary adenoid cystic carcinoma sacc |
| topic | Salivary adenoid cystic carcinoma PIM1 p21 RUNX3 Short hairpin RNA |
| url | http://link.springer.com/article/10.1186/s12935-018-0518-y |
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