Pyruvate Kinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigera
Pyruvate kinase (PYK) is a speed-limited enzyme of glycolysis that catalyzes the formation of pyruvate, and plays an important role in acetyl-CoA synthesis. The acetyl-CoA is the precursor of sex pheromone biosynthesis in Helicoverpa armigera. However, the role of PYK in sex pheromone biosynthesis r...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2021.707389/full |
| _version_ | 1819145050794229760 |
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| author | Shuangyan Yao Yunhui Zhang Yanpeng Chang Xiang Li Wenli Zhao Shiheng An |
| author_facet | Shuangyan Yao Yunhui Zhang Yanpeng Chang Xiang Li Wenli Zhao Shiheng An |
| author_sort | Shuangyan Yao |
| collection | DOAJ |
| description | Pyruvate kinase (PYK) is a speed-limited enzyme of glycolysis that catalyzes the formation of pyruvate, and plays an important role in acetyl-CoA synthesis. The acetyl-CoA is the precursor of sex pheromone biosynthesis in Helicoverpa armigera. However, the role of PYK in sex pheromone biosynthesis remains elusive. Here, PYK in H. armigera (HaPYK) was found to be highly expressed in the pheromone glands (PGs). The developmental expression profile of HaPYK was consistent with the fluctuation of sex pheromone release. Function analysis revealed that the knockdown of HaPYK led to a decrease in the levels of pyruvic acid and acetyl-CoA in PGs, which in turn caused a significant decrease in cis-11-hexadecenal (Z11-16: Ald) production, female capability to attract males, and mating frequency. Further study demonstrated that sugar feeding (5% sugar) increased the transcription and enzyme activity of HaPYK, thereby facilitating sex pheromone biosynthesis. Moreover, pheromone biosynthesis activating neuropeptide (PBAN) upregulated HaPYK activity through protein kinase C (PKC), as shown by PKC-specific inhibitor analysis. Altogether, our results revealed that PBAN activated HaPYK by Ca2+/PKC, thereby regulating the synthesis of pyruvate and subsequent acetyl-CoA, ensuring the supply of sex pheromone precursor, and finally facilitating sex pheromone biosynthesis and mating behavior. |
| first_indexed | 2024-12-22T12:51:52Z |
| format | Article |
| id | doaj.art-9a978b5f9ff44dc5af785092a2ad5f77 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-22T12:51:52Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-9a978b5f9ff44dc5af785092a2ad5f772022-12-21T18:25:12ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-08-011210.3389/fphys.2021.707389707389Pyruvate Kinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigeraShuangyan YaoYunhui ZhangYanpeng ChangXiang LiWenli ZhaoShiheng AnPyruvate kinase (PYK) is a speed-limited enzyme of glycolysis that catalyzes the formation of pyruvate, and plays an important role in acetyl-CoA synthesis. The acetyl-CoA is the precursor of sex pheromone biosynthesis in Helicoverpa armigera. However, the role of PYK in sex pheromone biosynthesis remains elusive. Here, PYK in H. armigera (HaPYK) was found to be highly expressed in the pheromone glands (PGs). The developmental expression profile of HaPYK was consistent with the fluctuation of sex pheromone release. Function analysis revealed that the knockdown of HaPYK led to a decrease in the levels of pyruvic acid and acetyl-CoA in PGs, which in turn caused a significant decrease in cis-11-hexadecenal (Z11-16: Ald) production, female capability to attract males, and mating frequency. Further study demonstrated that sugar feeding (5% sugar) increased the transcription and enzyme activity of HaPYK, thereby facilitating sex pheromone biosynthesis. Moreover, pheromone biosynthesis activating neuropeptide (PBAN) upregulated HaPYK activity through protein kinase C (PKC), as shown by PKC-specific inhibitor analysis. Altogether, our results revealed that PBAN activated HaPYK by Ca2+/PKC, thereby regulating the synthesis of pyruvate and subsequent acetyl-CoA, ensuring the supply of sex pheromone precursor, and finally facilitating sex pheromone biosynthesis and mating behavior.https://www.frontiersin.org/articles/10.3389/fphys.2021.707389/fullHaPYKacetyl-CoAZ11-16: Aldmating behaviorPKCsugar feeding |
| spellingShingle | Shuangyan Yao Yunhui Zhang Yanpeng Chang Xiang Li Wenli Zhao Shiheng An Pyruvate Kinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigera Frontiers in Physiology HaPYK acetyl-CoA Z11-16: Ald mating behavior PKC sugar feeding |
| title | Pyruvate Kinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigera |
| title_full | Pyruvate Kinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigera |
| title_fullStr | Pyruvate Kinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigera |
| title_full_unstemmed | Pyruvate Kinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigera |
| title_short | Pyruvate Kinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigera |
| title_sort | pyruvate kinase is required for sex pheromone biosynthesis in helicoverpa armigera |
| topic | HaPYK acetyl-CoA Z11-16: Ald mating behavior PKC sugar feeding |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2021.707389/full |
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