3D bioprinting of nanoparticle-laden hydrogel scaffolds with enhanced antibacterial and imaging properties
Summary: Biomaterial-associated microbial contaminations in biologically conducive three-dimensional (3D) tissue-engineered constructs have significantly limited the clinical applications of scaffold systems. To prevent such infections, antimicrobial biomaterials are rapidly evolving. Yet, the use o...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222012196 |
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| author | Andrea S. Theus Liqun Ning Gabriella Kabboul Boeun Hwang Martin L. Tomov Christopher N. LaRock Holly Bauser-Heaton Morteza Mahmoudi Vahid Serpooshan |
| author_facet | Andrea S. Theus Liqun Ning Gabriella Kabboul Boeun Hwang Martin L. Tomov Christopher N. LaRock Holly Bauser-Heaton Morteza Mahmoudi Vahid Serpooshan |
| author_sort | Andrea S. Theus |
| collection | DOAJ |
| description | Summary: Biomaterial-associated microbial contaminations in biologically conducive three-dimensional (3D) tissue-engineered constructs have significantly limited the clinical applications of scaffold systems. To prevent such infections, antimicrobial biomaterials are rapidly evolving. Yet, the use of such materials in bioprinting-based approaches of scaffold fabrication has not been examined. This study introduces a new generation of bacteriostatic gelatin methacryloyl (GelMA)-based bioinks, incorporated with varying doses of antibacterial superparamagnetic iron oxide nanoparticles (SPIONs). The SPION-laden GelMA scaffolds showed significant resistance against the Staphylococcus aureus growth, while providing a contrast in magnetic resonance imaging. We simulated the bacterial contamination of cellular 3D GelMA scaffolds in vitro and demonstrated the significant effect of functionalized scaffolds in inhibiting bacterial growth, while maintaining cell viability and growth. Together, these results present a new promising class of functionalized bioinks to 3D bioprint tissue-engineered scaffold with markedly enhanced properties for the use in a variety of in vitro and clinical applications. |
| first_indexed | 2024-04-13T01:27:53Z |
| format | Article |
| id | doaj.art-9a979f2b95d94671b143359e525d49bf |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-13T01:27:53Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-9a979f2b95d94671b143359e525d49bf2022-12-22T03:08:35ZengElsevieriScience2589-00422022-09-012591049473D bioprinting of nanoparticle-laden hydrogel scaffolds with enhanced antibacterial and imaging propertiesAndrea S. Theus0Liqun Ning1Gabriella Kabboul2Boeun Hwang3Martin L. Tomov4Christopher N. LaRock5Holly Bauser-Heaton6Morteza Mahmoudi7Vahid Serpooshan8Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USADepartment of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA; Emory Antibiotic Resistance Center, Emory University School of Medicine, Atlanta, GA 30322, USA; Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Children’s Healthcare of Atlanta, Atlanta, GA 30322, USA; Sibley Heart Center at Children’s Healthcare of Atlanta, Atlanta, GA 30342, USAPrecision Health Program, Michigan State University, East Lansing, MI 48842, USADepartment of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA 30322, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA; Children’s Healthcare of Atlanta, Atlanta, GA 30322, USA; Corresponding authorSummary: Biomaterial-associated microbial contaminations in biologically conducive three-dimensional (3D) tissue-engineered constructs have significantly limited the clinical applications of scaffold systems. To prevent such infections, antimicrobial biomaterials are rapidly evolving. Yet, the use of such materials in bioprinting-based approaches of scaffold fabrication has not been examined. This study introduces a new generation of bacteriostatic gelatin methacryloyl (GelMA)-based bioinks, incorporated with varying doses of antibacterial superparamagnetic iron oxide nanoparticles (SPIONs). The SPION-laden GelMA scaffolds showed significant resistance against the Staphylococcus aureus growth, while providing a contrast in magnetic resonance imaging. We simulated the bacterial contamination of cellular 3D GelMA scaffolds in vitro and demonstrated the significant effect of functionalized scaffolds in inhibiting bacterial growth, while maintaining cell viability and growth. Together, these results present a new promising class of functionalized bioinks to 3D bioprint tissue-engineered scaffold with markedly enhanced properties for the use in a variety of in vitro and clinical applications.http://www.sciencedirect.com/science/article/pii/S2589004222012196BiomaterialsNanoparticlesTissue engineering |
| spellingShingle | Andrea S. Theus Liqun Ning Gabriella Kabboul Boeun Hwang Martin L. Tomov Christopher N. LaRock Holly Bauser-Heaton Morteza Mahmoudi Vahid Serpooshan 3D bioprinting of nanoparticle-laden hydrogel scaffolds with enhanced antibacterial and imaging properties iScience Biomaterials Nanoparticles Tissue engineering |
| title | 3D bioprinting of nanoparticle-laden hydrogel scaffolds with enhanced antibacterial and imaging properties |
| title_full | 3D bioprinting of nanoparticle-laden hydrogel scaffolds with enhanced antibacterial and imaging properties |
| title_fullStr | 3D bioprinting of nanoparticle-laden hydrogel scaffolds with enhanced antibacterial and imaging properties |
| title_full_unstemmed | 3D bioprinting of nanoparticle-laden hydrogel scaffolds with enhanced antibacterial and imaging properties |
| title_short | 3D bioprinting of nanoparticle-laden hydrogel scaffolds with enhanced antibacterial and imaging properties |
| title_sort | 3d bioprinting of nanoparticle laden hydrogel scaffolds with enhanced antibacterial and imaging properties |
| topic | Biomaterials Nanoparticles Tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222012196 |
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