Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics
Probiotics have been used in human and veterinary medicine to increase resistance to pathogens and provide protection against external impacts for many years. Pathogens are often transmitted to humans through animal product consumption. Therefore, it is assumed that probiotics protecting animals may...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/3/910 |
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| author | Nicole Fülöpová Natália Chomová Jan Elbl Dagmar Mudroňová Patrik Sivulič Sylvie Pavloková Aleš Franc |
| author_facet | Nicole Fülöpová Natália Chomová Jan Elbl Dagmar Mudroňová Patrik Sivulič Sylvie Pavloková Aleš Franc |
| author_sort | Nicole Fülöpová |
| collection | DOAJ |
| description | Probiotics have been used in human and veterinary medicine to increase resistance to pathogens and provide protection against external impacts for many years. Pathogens are often transmitted to humans through animal product consumption. Therefore, it is assumed that probiotics protecting animals may also protect the humans who consume them. Many tested strains of probiotic bacteria can be used for individualized therapy. The recently isolated <i>Lactobacillus plantarum</i> R2 Biocenol™ has proven to be preferential in aquaculture, and potential benefits in humans are expected. A simple oral dosage form should be developed to test this hypothesis by a suitable preparation method, i.e., lyophilization, allowing the bacteria to survive longer. Lyophilizates were formed from silicates (Neusilin<sup>®</sup> NS2N; US2), cellulose derivates (Avicel<sup>®</sup> PH-101), and saccharides (inulin; saccharose; modified starch<sup>®</sup> 1500). They were evaluated for their physicochemical properties (pH leachate, moisture content, water absorption, wetting time, DSC tests, densities, and flow properties); their bacterial viability was determined in conditions including relevant studies over 6 months at 4 °C and scanned under an electron microscope. Lyophilizate composed of Neusilin<sup>®</sup> NS2N and saccharose appeared to be the most advantageous in terms of viability without any significant decrease. Its physicochemical properties are also suitable for capsule encapsulation, subsequent clinical evaluation, and individualized therapy. |
| first_indexed | 2024-03-11T06:02:26Z |
| format | Article |
| id | doaj.art-9a98aeaea1884edaa95d94d72f2ce37c |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T06:02:26Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-9a98aeaea1884edaa95d94d72f2ce37c2023-11-17T13:16:07ZengMDPI AGPharmaceutics1999-49232023-03-0115391010.3390/pharmaceutics15030910Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized ProbioticsNicole Fülöpová0Natália Chomová1Jan Elbl2Dagmar Mudroňová3Patrik Sivulič4Sylvie Pavloková5Aleš Franc6Department of Pharmaceutical Technology, Faculty of Pharmacy, Masaryk University, 612 42 Brno, Czech RepublicDepartment of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, SlovakiaDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Masaryk University, 612 42 Brno, Czech RepublicDepartment of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, SlovakiaDepartment of Natural Drugs, Faculty of Pharmacy, Masaryk University, 612 42 Brno, Czech RepublicDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Masaryk University, 612 42 Brno, Czech RepublicDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Masaryk University, 612 42 Brno, Czech RepublicProbiotics have been used in human and veterinary medicine to increase resistance to pathogens and provide protection against external impacts for many years. Pathogens are often transmitted to humans through animal product consumption. Therefore, it is assumed that probiotics protecting animals may also protect the humans who consume them. Many tested strains of probiotic bacteria can be used for individualized therapy. The recently isolated <i>Lactobacillus plantarum</i> R2 Biocenol™ has proven to be preferential in aquaculture, and potential benefits in humans are expected. A simple oral dosage form should be developed to test this hypothesis by a suitable preparation method, i.e., lyophilization, allowing the bacteria to survive longer. Lyophilizates were formed from silicates (Neusilin<sup>®</sup> NS2N; US2), cellulose derivates (Avicel<sup>®</sup> PH-101), and saccharides (inulin; saccharose; modified starch<sup>®</sup> 1500). They were evaluated for their physicochemical properties (pH leachate, moisture content, water absorption, wetting time, DSC tests, densities, and flow properties); their bacterial viability was determined in conditions including relevant studies over 6 months at 4 °C and scanned under an electron microscope. Lyophilizate composed of Neusilin<sup>®</sup> NS2N and saccharose appeared to be the most advantageous in terms of viability without any significant decrease. Its physicochemical properties are also suitable for capsule encapsulation, subsequent clinical evaluation, and individualized therapy.https://www.mdpi.com/1999-4923/15/3/910lyophilizationantropozoonosesprobiotic bacteriaindividual treatmentprincipal component analysisviability of bacteria |
| spellingShingle | Nicole Fülöpová Natália Chomová Jan Elbl Dagmar Mudroňová Patrik Sivulič Sylvie Pavloková Aleš Franc Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics Pharmaceutics lyophilization antropozoonoses probiotic bacteria individual treatment principal component analysis viability of bacteria |
| title | Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics |
| title_full | Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics |
| title_fullStr | Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics |
| title_full_unstemmed | Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics |
| title_short | Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics |
| title_sort | preparation and evaluation of a dosage form for individualized administration of lyophilized probiotics |
| topic | lyophilization antropozoonoses probiotic bacteria individual treatment principal component analysis viability of bacteria |
| url | https://www.mdpi.com/1999-4923/15/3/910 |
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