Invitro rearing of parasitoids

Since 2015, the studies on in vitro rearing Braconidae and Trichogrammatidae (B. habetor Say, B. greeni and T. pintoe, T. evanescens, T. chilonis) have been conducted successfully in Uzbekistan. For these parasites, cooking and the three main types of media were made from the hemolymph of the wax mo...

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Bibliographic Details
Main Author: Jumaev Rasul
Format: Article
Language:English
Published: EDP Sciences 2023-01-01
Series:E3S Web of Conferences
Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/08/e3sconf_afe2023_01032.pdf
Description
Summary:Since 2015, the studies on in vitro rearing Braconidae and Trichogrammatidae (B. habetor Say, B. greeni and T. pintoe, T. evanescens, T. chilonis) have been conducted successfully in Uzbekistan. For these parasites, cooking and the three main types of media were made from the hemolymph of the wax moth (G. melonnella) and cotton worm Helicoverpa armigera (Heliothis armigera), egg yolk, natural milk 10%. The simulated "host-eggs" and "larvae" are made of polyethylene or polypropylene semi spherical capsules, containing artificial diets, in which insectan pupal hemolymph is the main component. Mass production of in vitro reared Braconidae and Trichogrammatidae its utilization in the fields showed good effectiveness in controlling cotton bollworm. In the article researches were given about creating artificial medium of diets in order to grow bracon and choosing effective medium of diets. In our researches medium of diets which consists of some components was created and they were harmed with bracon generations. According to it, generation of bracon’s nutrition and developing three main types of medium were produced in order to rear bracon parasite that was composed wax moths (G.melonnellan) and (heliothis armigera) hemolymph, egg yolk, natural milk.
ISSN:2267-1242