In vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834
Background & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying...
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| Format: | Article |
| Language: | English |
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Islamic Azad University, Shahrekord Branch
2017-07-01
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| Series: | Journal of Herbal Drugs |
| Subjects: | |
| Online Access: | http://jhd.iaushk.ac.ir/article_25641.html |
| _version_ | 1827612540450897920 |
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| author | Ofelia Banihashemi Ramazan-Ali Khavari-Nejad Narguess Yassa Farzaneh Najafi |
| author_facet | Ofelia Banihashemi Ramazan-Ali Khavari-Nejad Narguess Yassa Farzaneh Najafi |
| author_sort | Ofelia Banihashemi |
| collection | DOAJ |
| description | Background & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture.
Experimental: The Atropa komarovii leaves were wounded and infected with soil gram-negative bacterium Agrobacterium rhizogenes ATCC15834. After three weeks, the transformation roots and control roots without infection, appeared, and for confirming that T-DNA Ri plasmid fragments were transformed and integrated to plant genome, the rolB gene region, was amplified using PCR. HPLC method was then used for assaying how two tropane alkaloids such as atropine (hyosciamine) and scopolamine (hyoscine) were produced in hairy roots,control roots, leaves and roots of plantlet.
Results: The data indicated that diagnostic 500bp rol B product amplification was exhibited to be present by all the transformed hairy roots. Scopolamine content in hairy roots was considerably greater than that in control roots but greatest (Hyoscyamine) atropine content was observed in control roots. Analysis of DW, FW and root length showed that fresh and dry root weight increased in hairy roots compared with that in non transformed root.
Recommended applications/industries: The present study demonstrated that secondary metabolite production using medicinal plants concerns many researchers worldwide today and hairy root culture is a useful method for producing tropane alkaloids in solanaceae. |
| first_indexed | 2024-03-09T08:24:46Z |
| format | Article |
| id | doaj.art-9aa2a225d1404c63b56739a6f6c3b00f |
| institution | Directory Open Access Journal |
| issn | 2008-8884 2476-5392 |
| language | English |
| last_indexed | 2024-03-09T08:24:46Z |
| publishDate | 2017-07-01 |
| publisher | Islamic Azad University, Shahrekord Branch |
| record_format | Article |
| series | Journal of Herbal Drugs |
| spelling | doaj.art-9aa2a225d1404c63b56739a6f6c3b00f2023-12-02T21:24:20ZengIslamic Azad University, Shahrekord BranchJournal of Herbal Drugs2008-88842476-53922017-07-0181515810.18869/JHD.2017.51In vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834Ofelia Banihashemi0Ramazan-Ali Khavari-Nejad1Narguess Yassa2Farzaneh Najafi3Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medicinal Sciences, Tehran, IranDepartment of Plant Science, Faculty of Biological Science, Kharazmi University, Tehran, IranBackground & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The Atropa komarovii leaves were wounded and infected with soil gram-negative bacterium Agrobacterium rhizogenes ATCC15834. After three weeks, the transformation roots and control roots without infection, appeared, and for confirming that T-DNA Ri plasmid fragments were transformed and integrated to plant genome, the rolB gene region, was amplified using PCR. HPLC method was then used for assaying how two tropane alkaloids such as atropine (hyosciamine) and scopolamine (hyoscine) were produced in hairy roots,control roots, leaves and roots of plantlet. Results: The data indicated that diagnostic 500bp rol B product amplification was exhibited to be present by all the transformed hairy roots. Scopolamine content in hairy roots was considerably greater than that in control roots but greatest (Hyoscyamine) atropine content was observed in control roots. Analysis of DW, FW and root length showed that fresh and dry root weight increased in hairy roots compared with that in non transformed root. Recommended applications/industries: The present study demonstrated that secondary metabolite production using medicinal plants concerns many researchers worldwide today and hairy root culture is a useful method for producing tropane alkaloids in solanaceae.http://jhd.iaushk.ac.ir/article_25641.htmlAtropa komaroviimedicinal plantrolBtransformationMedicinal plantTransformationTropane alkaloids |
| spellingShingle | Ofelia Banihashemi Ramazan-Ali Khavari-Nejad Narguess Yassa Farzaneh Najafi In vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834 Journal of Herbal Drugs Atropa komarovii medicinal plant rolB transformation Medicinal plant Transformation Tropane alkaloids |
| title | In vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834 |
| title_full | In vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834 |
| title_fullStr | In vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834 |
| title_full_unstemmed | In vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834 |
| title_short | In vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834 |
| title_sort | in vitro production of secondary metabolite using atropa komarovii bline shal solanaceae hairy root culture via agrobacterium rhizogenes atcc15834 |
| topic | Atropa komarovii medicinal plant rolB transformation Medicinal plant Transformation Tropane alkaloids |
| url | http://jhd.iaushk.ac.ir/article_25641.html |
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