IN-VITRO STUDY OF SUB-LETHAL EFFECT OF NEW CHEMISTRY INSECTICIDES ON THE ADULT CHRYSOPERLA CARNEA (STEPHENS)

Four new chemistry insecticides viz. Bifenthrin (Talstar® 10% EC, FMC), Acetamiprid (Acelan®,20% EC, FMC), Imidacloprid (Imidacloprid®,25% EC, FMC) and Diafenthiuron (Polo®,100% EC, Syngenta Company) were evaluated against Chrysoperla carnea. Different concentrations (1.25, 2.5, 5, 7.5 and 10 µl/ml...

Full description

Bibliographic Details
Main Authors: Q. A. Soomro, R. Sultana, R. Muhammad, M. Sohail, N. H. Khuhro
Format: Article
Language:English
Published: Sindh Agriculture University, Tandojam 2019-06-01
Series:Pakistan Journal of Agriculture, Agricultural Engineering & Veterinary Sciences
Subjects:
Online Access:https://pjaaevs.sau.edu.pk/index.php/ojs/article/view/312
_version_ 1797777359045132288
author Q. A. Soomro
R. Sultana
R. Muhammad
M. Sohail
N. H. Khuhro
author_facet Q. A. Soomro
R. Sultana
R. Muhammad
M. Sohail
N. H. Khuhro
author_sort Q. A. Soomro
collection DOAJ
description Four new chemistry insecticides viz. Bifenthrin (Talstar® 10% EC, FMC), Acetamiprid (Acelan®,20% EC, FMC), Imidacloprid (Imidacloprid®,25% EC, FMC) and Diafenthiuron (Polo®,100% EC, Syngenta Company) were evaluated against Chrysoperla carnea. Different concentrations (1.25, 2.5, 5, 7.5 and 10 µl/ml) of each insecticide were made in distilled water by serial dilutions tested to determine the sub-lethal concentration dose LC50 against adult’s C. carnea under laboratory conditions. Topical bioassay was performed against adult C. carnea. Adulticidal activity of insecticide in term of LC50 and LC90 with confidential interval was evaluated. The LC50 values of Acetamiprid, Bifenthrin, Imidacloprid and Diafenthiuron for adults C. carnea were 31.3, 96.1, 2.3 and 30.5 µl/ml, respectively. Toxicity of the tested insecticides to C. carnea form most to least toxic was Imidacloprid > Diafenthiuron > Acetamiprid > Bifenthrin. The 95% confidence intervals of Acetamiprid, Bifenthrin and Diafenthiuron overlapped, suggesting no significant difference in the toxicities of these three insecticides to the adult C. carnea. The adults were most sensitive to Imidacloprid and required low concentration (2.3 ± 0.25 µl/ml) to cause 50% mortality of the targeted adults compared to rest of the insecticide. Bifenthrin was found to be most tolerant and require high concentration to acquire LC50 that is 96.1± 28.6 µl/ml. The log concentration under directly proportional to the probability of adult C. carnea mortality. Increase in log concentration of Acetamiprid (0.894) resulted increase in probability (3.66). The decrease in the log concentration (0.854) of Bifenthrin decreased the (3.31) probability of mortality. Similarly the log concentration related to probability of mortality with the increase of log concentration (2.453) of Imidacloprid increased the probability (4.12). While the proportion of decreased log concentration of Polo (1.206) decreased the probability (3.120). The finding of this study proved that the above tested insecticides are harmful to adult of C. carnea. Generaly Bifenthrin causes less damage and may be included in the Integrated Pest Management (IPM) program with fairly adverse residual effect on bio-control agents.
first_indexed 2024-03-12T23:03:53Z
format Article
id doaj.art-9aa65218cbb14f2aa4a6268cc08ee85f
institution Directory Open Access Journal
issn 1023-1072
2663-7863
language English
last_indexed 2024-03-12T23:03:53Z
publishDate 2019-06-01
publisher Sindh Agriculture University, Tandojam
record_format Article
series Pakistan Journal of Agriculture, Agricultural Engineering & Veterinary Sciences
spelling doaj.art-9aa65218cbb14f2aa4a6268cc08ee85f2023-07-19T07:44:42ZengSindh Agriculture University, TandojamPakistan Journal of Agriculture, Agricultural Engineering & Veterinary Sciences1023-10722663-78632019-06-0135(1) JuneIN-VITRO STUDY OF SUB-LETHAL EFFECT OF NEW CHEMISTRY INSECTICIDES ON THE ADULT CHRYSOPERLA CARNEA (STEPHENS)Q. A. Soomro0R. Sultana1R. Muhammad2M. Sohail3N. H. Khuhro4Plant Protection Division, Nuclear Institute of Agriculture, Tandojam, PakistanDepartment of Zoology, University of Sindh, Jamshoro, PakistanPlant Protection Division, Nuclear Institute of Agriculture, Tandojam, PakistanDepartment of Entomology, University College of Agriculture, University of Sargodha, PakistanPlant Protection Division, Nuclear Institute of Agriculture, Tandojam, Pakistan Four new chemistry insecticides viz. Bifenthrin (Talstar® 10% EC, FMC), Acetamiprid (Acelan®,20% EC, FMC), Imidacloprid (Imidacloprid®,25% EC, FMC) and Diafenthiuron (Polo®,100% EC, Syngenta Company) were evaluated against Chrysoperla carnea. Different concentrations (1.25, 2.5, 5, 7.5 and 10 µl/ml) of each insecticide were made in distilled water by serial dilutions tested to determine the sub-lethal concentration dose LC50 against adult’s C. carnea under laboratory conditions. Topical bioassay was performed against adult C. carnea. Adulticidal activity of insecticide in term of LC50 and LC90 with confidential interval was evaluated. The LC50 values of Acetamiprid, Bifenthrin, Imidacloprid and Diafenthiuron for adults C. carnea were 31.3, 96.1, 2.3 and 30.5 µl/ml, respectively. Toxicity of the tested insecticides to C. carnea form most to least toxic was Imidacloprid > Diafenthiuron > Acetamiprid > Bifenthrin. The 95% confidence intervals of Acetamiprid, Bifenthrin and Diafenthiuron overlapped, suggesting no significant difference in the toxicities of these three insecticides to the adult C. carnea. The adults were most sensitive to Imidacloprid and required low concentration (2.3 ± 0.25 µl/ml) to cause 50% mortality of the targeted adults compared to rest of the insecticide. Bifenthrin was found to be most tolerant and require high concentration to acquire LC50 that is 96.1± 28.6 µl/ml. The log concentration under directly proportional to the probability of adult C. carnea mortality. Increase in log concentration of Acetamiprid (0.894) resulted increase in probability (3.66). The decrease in the log concentration (0.854) of Bifenthrin decreased the (3.31) probability of mortality. Similarly the log concentration related to probability of mortality with the increase of log concentration (2.453) of Imidacloprid increased the probability (4.12). While the proportion of decreased log concentration of Polo (1.206) decreased the probability (3.120). The finding of this study proved that the above tested insecticides are harmful to adult of C. carnea. Generaly Bifenthrin causes less damage and may be included in the Integrated Pest Management (IPM) program with fairly adverse residual effect on bio-control agents. https://pjaaevs.sau.edu.pk/index.php/ojs/article/view/312adult mortalityChrysoperla carneainsecticidessub-lethal concentration
spellingShingle Q. A. Soomro
R. Sultana
R. Muhammad
M. Sohail
N. H. Khuhro
IN-VITRO STUDY OF SUB-LETHAL EFFECT OF NEW CHEMISTRY INSECTICIDES ON THE ADULT CHRYSOPERLA CARNEA (STEPHENS)
Pakistan Journal of Agriculture, Agricultural Engineering & Veterinary Sciences
adult mortality
Chrysoperla carnea
insecticides
sub-lethal concentration
title IN-VITRO STUDY OF SUB-LETHAL EFFECT OF NEW CHEMISTRY INSECTICIDES ON THE ADULT CHRYSOPERLA CARNEA (STEPHENS)
title_full IN-VITRO STUDY OF SUB-LETHAL EFFECT OF NEW CHEMISTRY INSECTICIDES ON THE ADULT CHRYSOPERLA CARNEA (STEPHENS)
title_fullStr IN-VITRO STUDY OF SUB-LETHAL EFFECT OF NEW CHEMISTRY INSECTICIDES ON THE ADULT CHRYSOPERLA CARNEA (STEPHENS)
title_full_unstemmed IN-VITRO STUDY OF SUB-LETHAL EFFECT OF NEW CHEMISTRY INSECTICIDES ON THE ADULT CHRYSOPERLA CARNEA (STEPHENS)
title_short IN-VITRO STUDY OF SUB-LETHAL EFFECT OF NEW CHEMISTRY INSECTICIDES ON THE ADULT CHRYSOPERLA CARNEA (STEPHENS)
title_sort in vitro study of sub lethal effect of new chemistry insecticides on the adult chrysoperla carnea stephens
topic adult mortality
Chrysoperla carnea
insecticides
sub-lethal concentration
url https://pjaaevs.sau.edu.pk/index.php/ojs/article/view/312
work_keys_str_mv AT qasoomro invitrostudyofsublethaleffectofnewchemistryinsecticidesontheadultchrysoperlacarneastephens
AT rsultana invitrostudyofsublethaleffectofnewchemistryinsecticidesontheadultchrysoperlacarneastephens
AT rmuhammad invitrostudyofsublethaleffectofnewchemistryinsecticidesontheadultchrysoperlacarneastephens
AT msohail invitrostudyofsublethaleffectofnewchemistryinsecticidesontheadultchrysoperlacarneastephens
AT nhkhuhro invitrostudyofsublethaleffectofnewchemistryinsecticidesontheadultchrysoperlacarneastephens