Single and repeated dose (28 days) intravenous toxicity assessment of bartogenic acid (an active pentacyclic triterpenoid) isolated from Barringtonia racemosa (L.) fruits in mice
Bartogenic acid (BA), an active pentacyclic triterpenoid, has been reported for anti-diabetic, anti-inflammatory, anti-arthritic, anti-cancer, and anti-tumor activity. However, toxicity profiling of BA has not been reported till date. Hence, this study is designed to evaluate the single dose (12.5,...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-01-01
|
| Series: | Current Research in Toxicology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666027X21000384 |
| _version_ | 1797978776303304704 |
|---|---|
| author | Vishal Kumar Dubey Swati Madan Satyendra K. Rajput Anu T Singh Manu Jaggi Amit Kumar Mittal |
| author_facet | Vishal Kumar Dubey Swati Madan Satyendra K. Rajput Anu T Singh Manu Jaggi Amit Kumar Mittal |
| author_sort | Vishal Kumar Dubey |
| collection | DOAJ |
| description | Bartogenic acid (BA), an active pentacyclic triterpenoid, has been reported for anti-diabetic, anti-inflammatory, anti-arthritic, anti-cancer, and anti-tumor activity. However, toxicity profiling of BA has not been reported till date. Hence, this study is designed to evaluate the single dose (12.5, 25, 50 and 100 mg/kg) and repeated dose (1.5, 6, and 24 mg/kg) intravenous toxicity of BA in BALB/c mice. Control group received vehicle. In single dose toxicity study, two mortalities were observed at 100 mg/kg of BA whereas lower doses were well tolerated. In repeated dose toxicity study, no mortality was observed. 1.5 mg/kg of BA was well tolerated in mice of both sexes. At 6 mg/kg of BA, female mice showed significant reduction in the body weight as compared to the control group however no significant change was observed in male mice. 24 mg/kg of BA showed significant reduction in the body weight in mice of both sexes. Further, these mice showed significant change in the relative organ weight. However, no toxicologically relevant changes were observed in hematology, biochemistry, and histopathology. Based on the findings, No-Observed-Adverse-Effect-Level (NOAEL) for BA were found to be<24 mg/kg for male mice and<6 mg/kg for female mice. |
| first_indexed | 2024-04-11T05:28:24Z |
| format | Article |
| id | doaj.art-9aa8332f1561430593e50972f022b3a7 |
| institution | Directory Open Access Journal |
| issn | 2666-027X |
| language | English |
| last_indexed | 2024-04-11T05:28:24Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Toxicology |
| spelling | doaj.art-9aa8332f1561430593e50972f022b3a72022-12-23T04:42:08ZengElsevierCurrent Research in Toxicology2666-027X2022-01-013100057Single and repeated dose (28 days) intravenous toxicity assessment of bartogenic acid (an active pentacyclic triterpenoid) isolated from Barringtonia racemosa (L.) fruits in miceVishal Kumar Dubey0Swati Madan1Satyendra K. Rajput2Anu T Singh3Manu Jaggi4Amit Kumar Mittal5Dabur Research Foundation, 22, Site IV, Sahibabad, Ghaziabad 201010, Uttar Pradesh, India; Amity Institute of Pharmacy, Amity University Uttar Pradesh, Sector-125, Noida 201313, Uttar Pradesh, India; Corresponding author at: Dabur Research Foundation, 22, Site IV, Sahibabad, Ghaziabad 201010, Uttar Pradesh, IndiaAmity Institute of Pharmacy, Amity University Uttar Pradesh, Sector-125, Noida 201313, Uttar Pradesh, IndiaDepartment of Pharmaceutical Sciences, Gurukula Kangri Vishwavidyalaya, Jagjeetpur, Haridwar 249404, Uttarakhand, IndiaDabur Research Foundation, 22, Site IV, Sahibabad, Ghaziabad 201010, Uttar Pradesh, IndiaDabur Research Foundation, 22, Site IV, Sahibabad, Ghaziabad 201010, Uttar Pradesh, IndiaAmity Institute of Pharmacy, Amity University Noida, Uttar Pradesh, 322230 IndiaBartogenic acid (BA), an active pentacyclic triterpenoid, has been reported for anti-diabetic, anti-inflammatory, anti-arthritic, anti-cancer, and anti-tumor activity. However, toxicity profiling of BA has not been reported till date. Hence, this study is designed to evaluate the single dose (12.5, 25, 50 and 100 mg/kg) and repeated dose (1.5, 6, and 24 mg/kg) intravenous toxicity of BA in BALB/c mice. Control group received vehicle. In single dose toxicity study, two mortalities were observed at 100 mg/kg of BA whereas lower doses were well tolerated. In repeated dose toxicity study, no mortality was observed. 1.5 mg/kg of BA was well tolerated in mice of both sexes. At 6 mg/kg of BA, female mice showed significant reduction in the body weight as compared to the control group however no significant change was observed in male mice. 24 mg/kg of BA showed significant reduction in the body weight in mice of both sexes. Further, these mice showed significant change in the relative organ weight. However, no toxicologically relevant changes were observed in hematology, biochemistry, and histopathology. Based on the findings, No-Observed-Adverse-Effect-Level (NOAEL) for BA were found to be<24 mg/kg for male mice and<6 mg/kg for female mice.http://www.sciencedirect.com/science/article/pii/S2666027X21000384Bartogenic acidBarringtonia racemosaToxicityAcuteNOAELMice |
| spellingShingle | Vishal Kumar Dubey Swati Madan Satyendra K. Rajput Anu T Singh Manu Jaggi Amit Kumar Mittal Single and repeated dose (28 days) intravenous toxicity assessment of bartogenic acid (an active pentacyclic triterpenoid) isolated from Barringtonia racemosa (L.) fruits in mice Current Research in Toxicology Bartogenic acid Barringtonia racemosa Toxicity Acute NOAEL Mice |
| title | Single and repeated dose (28 days) intravenous toxicity assessment of bartogenic acid (an active pentacyclic triterpenoid) isolated from Barringtonia racemosa (L.) fruits in mice |
| title_full | Single and repeated dose (28 days) intravenous toxicity assessment of bartogenic acid (an active pentacyclic triterpenoid) isolated from Barringtonia racemosa (L.) fruits in mice |
| title_fullStr | Single and repeated dose (28 days) intravenous toxicity assessment of bartogenic acid (an active pentacyclic triterpenoid) isolated from Barringtonia racemosa (L.) fruits in mice |
| title_full_unstemmed | Single and repeated dose (28 days) intravenous toxicity assessment of bartogenic acid (an active pentacyclic triterpenoid) isolated from Barringtonia racemosa (L.) fruits in mice |
| title_short | Single and repeated dose (28 days) intravenous toxicity assessment of bartogenic acid (an active pentacyclic triterpenoid) isolated from Barringtonia racemosa (L.) fruits in mice |
| title_sort | single and repeated dose 28 days intravenous toxicity assessment of bartogenic acid an active pentacyclic triterpenoid isolated from barringtonia racemosa l fruits in mice |
| topic | Bartogenic acid Barringtonia racemosa Toxicity Acute NOAEL Mice |
| url | http://www.sciencedirect.com/science/article/pii/S2666027X21000384 |
| work_keys_str_mv | AT vishalkumardubey singleandrepeateddose28daysintravenoustoxicityassessmentofbartogenicacidanactivepentacyclictriterpenoidisolatedfrombarringtoniaracemosalfruitsinmice AT swatimadan singleandrepeateddose28daysintravenoustoxicityassessmentofbartogenicacidanactivepentacyclictriterpenoidisolatedfrombarringtoniaracemosalfruitsinmice AT satyendrakrajput singleandrepeateddose28daysintravenoustoxicityassessmentofbartogenicacidanactivepentacyclictriterpenoidisolatedfrombarringtoniaracemosalfruitsinmice AT anutsingh singleandrepeateddose28daysintravenoustoxicityassessmentofbartogenicacidanactivepentacyclictriterpenoidisolatedfrombarringtoniaracemosalfruitsinmice AT manujaggi singleandrepeateddose28daysintravenoustoxicityassessmentofbartogenicacidanactivepentacyclictriterpenoidisolatedfrombarringtoniaracemosalfruitsinmice AT amitkumarmittal singleandrepeateddose28daysintravenoustoxicityassessmentofbartogenicacidanactivepentacyclictriterpenoidisolatedfrombarringtoniaracemosalfruitsinmice |