The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain.
OBJECTIVE:Pain management is a huge challenge in the treatment of rheumatoid arthritis (RA), and central sensitization is reportedly involved in the development of pain. The current study was undertaken to explore the possible role of N-methyl-D-aspartate receptors (NMDARs) in the spinal mechanism o...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6053233?pdf=render |
| _version_ | 1811195002632536064 |
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| author | Yingming Xu Kui Zhang Jinlin Miao Peng Zhao Minghua Lv Jia Li Xianghui Fu Xing Luo Ping Zhu |
| author_facet | Yingming Xu Kui Zhang Jinlin Miao Peng Zhao Minghua Lv Jia Li Xianghui Fu Xing Luo Ping Zhu |
| author_sort | Yingming Xu |
| collection | DOAJ |
| description | OBJECTIVE:Pain management is a huge challenge in the treatment of rheumatoid arthritis (RA), and central sensitization is reportedly involved in the development of pain. The current study was undertaken to explore the possible role of N-methyl-D-aspartate receptors (NMDARs) in the spinal mechanism of central sensitization in RA using a collagen-induced arthritis (CIA) model. METHODS:Mechanical hypersensitivity was assessed in C57BL/6 mice, before and after the induction of CIA via administration of chick type II collagen. Analgesic drugs, receptor antagonist, and kinase inhibitor were administrated intrathecally in the spinal cord. Protein expression and phosphorylation changes were detected via immunoblotting. RESULTS:CIA mice developed significant mechanical hypersensitivity, and spinal administration of the NMDAR antagonist D-2-amino-5-phosphonovaleric acid (D-APV) effectively attenuated peripheral pain hypersensitivity. There was specific enhancement of synaptic NR2B-containing NMDAR (NR2BR) expression in the spinal dorsal horns of the mice. Both the increased total protein expression of NR2B subunit and the enhanced total phosphorylation level of NR2B subunit at 1472 tyrosine promoted the synaptic expression of NMDAR in the mice. Intrathecal injection of tramadol suppressed synaptic NMDAR expression mainly by changing the synaptic phosphorylation state of NR2B subunit at Tyr1472. Extracellular signal-regulated protein kinases 2 (ERK2) activity synchronized with the synaptic expression of NR2BR, which was downregulated by the action of tramadol. CONCLUSION:Specific enhancement of NR2BR in the spinal dorsal horn may be vital for central sensitization in the CIA model of RA. The NR2BR/ERK2 pathway may be a promising target for pain management in RA patients. |
| first_indexed | 2024-04-12T00:36:32Z |
| format | Article |
| id | doaj.art-9aaae5f96c9845568c5140a004db2087 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T00:36:32Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9aaae5f96c9845568c5140a004db20872022-12-22T03:55:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01137e020102110.1371/journal.pone.0201021The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain.Yingming XuKui ZhangJinlin MiaoPeng ZhaoMinghua LvJia LiXianghui FuXing LuoPing ZhuOBJECTIVE:Pain management is a huge challenge in the treatment of rheumatoid arthritis (RA), and central sensitization is reportedly involved in the development of pain. The current study was undertaken to explore the possible role of N-methyl-D-aspartate receptors (NMDARs) in the spinal mechanism of central sensitization in RA using a collagen-induced arthritis (CIA) model. METHODS:Mechanical hypersensitivity was assessed in C57BL/6 mice, before and after the induction of CIA via administration of chick type II collagen. Analgesic drugs, receptor antagonist, and kinase inhibitor were administrated intrathecally in the spinal cord. Protein expression and phosphorylation changes were detected via immunoblotting. RESULTS:CIA mice developed significant mechanical hypersensitivity, and spinal administration of the NMDAR antagonist D-2-amino-5-phosphonovaleric acid (D-APV) effectively attenuated peripheral pain hypersensitivity. There was specific enhancement of synaptic NR2B-containing NMDAR (NR2BR) expression in the spinal dorsal horns of the mice. Both the increased total protein expression of NR2B subunit and the enhanced total phosphorylation level of NR2B subunit at 1472 tyrosine promoted the synaptic expression of NMDAR in the mice. Intrathecal injection of tramadol suppressed synaptic NMDAR expression mainly by changing the synaptic phosphorylation state of NR2B subunit at Tyr1472. Extracellular signal-regulated protein kinases 2 (ERK2) activity synchronized with the synaptic expression of NR2BR, which was downregulated by the action of tramadol. CONCLUSION:Specific enhancement of NR2BR in the spinal dorsal horn may be vital for central sensitization in the CIA model of RA. The NR2BR/ERK2 pathway may be a promising target for pain management in RA patients.http://europepmc.org/articles/PMC6053233?pdf=render |
| spellingShingle | Yingming Xu Kui Zhang Jinlin Miao Peng Zhao Minghua Lv Jia Li Xianghui Fu Xing Luo Ping Zhu The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain. PLoS ONE |
| title | The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain. |
| title_full | The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain. |
| title_fullStr | The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain. |
| title_full_unstemmed | The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain. |
| title_short | The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain. |
| title_sort | spinal nr2br erk2 pathway as a target for the central sensitization of collagen induced arthritis pain |
| url | http://europepmc.org/articles/PMC6053233?pdf=render |
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