Positive Microbiological Culture among Patients with Infective Keratitis Visiting the Cornea Unit of a Tertiary Care Centre
Introduction: Keratitis is the infection and inflammation of the cornea. Microbial keratitis is a potentially sight-threatening corneal condition. The aim of this study was to find out the prevalence of positive microbiological culture among patients with infective keratitis visiting the Cornea Uni...
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| Format: | Article |
| Language: | English |
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Nepal Medical Association
2023-10-01
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| Series: | Journal of Nepal Medical Association |
| Subjects: | |
| Online Access: | https://jnma.com.np/jnma/index.php/jnma/article/view/8285 |
| _version_ | 1797663552210731008 |
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| author | Leesha Shrestha Joshi Raju Kaiti Ranjila Shyangbo Birkha Bogati |
| author_facet | Leesha Shrestha Joshi Raju Kaiti Ranjila Shyangbo Birkha Bogati |
| author_sort | Leesha Shrestha Joshi |
| collection | DOAJ |
| description |
Introduction: Keratitis is the infection and inflammation of the cornea. Microbial keratitis is a potentially sight-threatening corneal condition. The aim of this study was to find out the prevalence of positive microbiological culture among patients with infective keratitis visiting the Cornea Unit of a tertiary care centre.
Methods: A descriptive cross-sectional study was conducted among patients with a clinical diagnosis of infective keratitis presenting to the Cornea Unit of a tertiary eye care centre from 16 October 2020 to 16 March 2021 after obtaining ethical approval from the Ethical Review Board. After slit-lamp examination, corneal scrapings were performed under aseptic conditions which were subjected to Gram stain, potassium hydroxide preparation and culture for bacterial and fungal pathogens. A convenience sampling method was used. The point estimate was calculated at a 95% Confidence Interval.
Results: Among 428 patients, 337 (78.73%) (73.24-84.33, 95% Confidence Interval) had a positive microbiological culture. A total of 213 (49.76%) of enrolled samples had a prior history of ocular trauma. Aspergillus species 68 (20.17%) and Streptococcus species 33 (9.79%) were the most common organisms isolated from fungal and bacterial corneal ulcers respectively.
Conclusions: The prevalence of positive microbiological culture among patients with infective keratitis from this study is similar to the pattern reported from similar settings.
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| first_indexed | 2024-03-11T19:16:20Z |
| format | Article |
| id | doaj.art-9aab40a2e7264a73ae99cfef922c9872 |
| institution | Directory Open Access Journal |
| issn | 0028-2715 1815-672X |
| language | English |
| last_indexed | 2024-03-11T19:16:20Z |
| publishDate | 2023-10-01 |
| publisher | Nepal Medical Association |
| record_format | Article |
| series | Journal of Nepal Medical Association |
| spelling | doaj.art-9aab40a2e7264a73ae99cfef922c98722023-10-09T05:03:44ZengNepal Medical AssociationJournal of Nepal Medical Association0028-27151815-672X2023-10-016126610.31729/jnma.8285Positive Microbiological Culture among Patients with Infective Keratitis Visiting the Cornea Unit of a Tertiary Care CentreLeesha Shrestha Joshi0Raju Kaiti1Ranjila Shyangbo2Birkha Bogati3Department of Ophthalmology, Nepal Eye Hospital, Tripureshwor, Kathmandu, NepalDepartment of Optometry, Nepal Eye Hospital, Tripureshwor, Kathmandu, NepalDepartment of Optometry, National Academy of Medical Sciences, Mahaboudha, Kathmandu, NepalDepartment of Optometry, National Academy of Medical Sciences, Mahaboudha, Kathmandu, Nepal Introduction: Keratitis is the infection and inflammation of the cornea. Microbial keratitis is a potentially sight-threatening corneal condition. The aim of this study was to find out the prevalence of positive microbiological culture among patients with infective keratitis visiting the Cornea Unit of a tertiary care centre. Methods: A descriptive cross-sectional study was conducted among patients with a clinical diagnosis of infective keratitis presenting to the Cornea Unit of a tertiary eye care centre from 16 October 2020 to 16 March 2021 after obtaining ethical approval from the Ethical Review Board. After slit-lamp examination, corneal scrapings were performed under aseptic conditions which were subjected to Gram stain, potassium hydroxide preparation and culture for bacterial and fungal pathogens. A convenience sampling method was used. The point estimate was calculated at a 95% Confidence Interval. Results: Among 428 patients, 337 (78.73%) (73.24-84.33, 95% Confidence Interval) had a positive microbiological culture. A total of 213 (49.76%) of enrolled samples had a prior history of ocular trauma. Aspergillus species 68 (20.17%) and Streptococcus species 33 (9.79%) were the most common organisms isolated from fungal and bacterial corneal ulcers respectively. Conclusions: The prevalence of positive microbiological culture among patients with infective keratitis from this study is similar to the pattern reported from similar settings. https://jnma.com.np/jnma/index.php/jnma/article/view/8285corneal ulcerkeratitisprevalence |
| spellingShingle | Leesha Shrestha Joshi Raju Kaiti Ranjila Shyangbo Birkha Bogati Positive Microbiological Culture among Patients with Infective Keratitis Visiting the Cornea Unit of a Tertiary Care Centre Journal of Nepal Medical Association corneal ulcer keratitis prevalence |
| title | Positive Microbiological Culture among Patients with Infective Keratitis Visiting the Cornea Unit of a Tertiary Care Centre |
| title_full | Positive Microbiological Culture among Patients with Infective Keratitis Visiting the Cornea Unit of a Tertiary Care Centre |
| title_fullStr | Positive Microbiological Culture among Patients with Infective Keratitis Visiting the Cornea Unit of a Tertiary Care Centre |
| title_full_unstemmed | Positive Microbiological Culture among Patients with Infective Keratitis Visiting the Cornea Unit of a Tertiary Care Centre |
| title_short | Positive Microbiological Culture among Patients with Infective Keratitis Visiting the Cornea Unit of a Tertiary Care Centre |
| title_sort | positive microbiological culture among patients with infective keratitis visiting the cornea unit of a tertiary care centre |
| topic | corneal ulcer keratitis prevalence |
| url | https://jnma.com.np/jnma/index.php/jnma/article/view/8285 |
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