Identification of a miRNA Panel with a Potential Determinant Role in Patients Suffering from Periodontitis
In recent years, the role of microRNA (miRNA) in post-transcriptional gene regulation has advanced and supports strong evidence related to their important role in the regulation of a wide range of fundamental biological processes. Our study focuses on identifying specific alterations of miRNA patter...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/3/145 |
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| author | Oana Baru Lajos Raduly Cecilia Bica Paul Chiroi Liviuta Budisan Nikolay Mehterov Cristina Ciocan Laura Ancuta Pop Smaranda Buduru Cornelia Braicu Mandra Badea Ioana Berindan-Neagoe |
| author_facet | Oana Baru Lajos Raduly Cecilia Bica Paul Chiroi Liviuta Budisan Nikolay Mehterov Cristina Ciocan Laura Ancuta Pop Smaranda Buduru Cornelia Braicu Mandra Badea Ioana Berindan-Neagoe |
| author_sort | Oana Baru |
| collection | DOAJ |
| description | In recent years, the role of microRNA (miRNA) in post-transcriptional gene regulation has advanced and supports strong evidence related to their important role in the regulation of a wide range of fundamental biological processes. Our study focuses on identifying specific alterations of miRNA patterns in periodontitis compared with healthy subjects. In the present study, we mapped the major miRNAs altered in patients with periodontitis (n = 3) compared with healthy subjects (n = 5), using microarray technology followed by a validation step by qRT-PCR and Ingenuity Pathways Analysis. Compared to healthy subjects, 159 differentially expressed miRNAs were identified among periodontitis patients, of which 89 were downregulated, and 70 were upregulated, considering a fold change of ±1.5 as the cut-off value and <i>p</i> ≤ 0.05. Key angiogenic miRNAs (miR-191-3p, miR-221-3p, miR-224-5p, miR-1228-3p) were further validated on a separate cohort of patients with periodontitis versus healthy controls by qRT-PCR, confirming the microarray data. Our findings indicate a periodontitis-specific miRNA expression pattern representing an essential issue for testing new potential diagnostic or prognostic biomarkers for periodontal disease. The identified miRNA profile in periodontal gingival tissue was linked to angiogenesis, with an important molecular mechanism that orchestrates cell fate. |
| first_indexed | 2024-03-11T06:43:56Z |
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| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T06:43:56Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-9aaf3a301909407196065827ca3e31692023-11-17T10:23:06ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-03-014532248226510.3390/cimb45030145Identification of a miRNA Panel with a Potential Determinant Role in Patients Suffering from PeriodontitisOana Baru0Lajos Raduly1Cecilia Bica2Paul Chiroi3Liviuta Budisan4Nikolay Mehterov5Cristina Ciocan6Laura Ancuta Pop7Smaranda Buduru8Cornelia Braicu9Mandra Badea10Ioana Berindan-Neagoe11Department of Preventive Dentistry, Faculty of Dental Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400083 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400015 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400015 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400015 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400015 Cluj-Napoca, RomaniaDepartment of Medical Biology, Medical University of Plovdiv, 4002 Plovdiv, BulgariaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400015 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400015 Cluj-Napoca, RomaniaStomestet Stomatology Clinic, Calea Manastur 68A Street, 400658 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400015 Cluj-Napoca, RomaniaDepartment of Preventive Dentistry, Faculty of Dental Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400083 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400015 Cluj-Napoca, RomaniaIn recent years, the role of microRNA (miRNA) in post-transcriptional gene regulation has advanced and supports strong evidence related to their important role in the regulation of a wide range of fundamental biological processes. Our study focuses on identifying specific alterations of miRNA patterns in periodontitis compared with healthy subjects. In the present study, we mapped the major miRNAs altered in patients with periodontitis (n = 3) compared with healthy subjects (n = 5), using microarray technology followed by a validation step by qRT-PCR and Ingenuity Pathways Analysis. Compared to healthy subjects, 159 differentially expressed miRNAs were identified among periodontitis patients, of which 89 were downregulated, and 70 were upregulated, considering a fold change of ±1.5 as the cut-off value and <i>p</i> ≤ 0.05. Key angiogenic miRNAs (miR-191-3p, miR-221-3p, miR-224-5p, miR-1228-3p) were further validated on a separate cohort of patients with periodontitis versus healthy controls by qRT-PCR, confirming the microarray data. Our findings indicate a periodontitis-specific miRNA expression pattern representing an essential issue for testing new potential diagnostic or prognostic biomarkers for periodontal disease. The identified miRNA profile in periodontal gingival tissue was linked to angiogenesis, with an important molecular mechanism that orchestrates cell fate.https://www.mdpi.com/1467-3045/45/3/145periodontitisaltered miRNA patternbiological networks |
| spellingShingle | Oana Baru Lajos Raduly Cecilia Bica Paul Chiroi Liviuta Budisan Nikolay Mehterov Cristina Ciocan Laura Ancuta Pop Smaranda Buduru Cornelia Braicu Mandra Badea Ioana Berindan-Neagoe Identification of a miRNA Panel with a Potential Determinant Role in Patients Suffering from Periodontitis Current Issues in Molecular Biology periodontitis altered miRNA pattern biological networks |
| title | Identification of a miRNA Panel with a Potential Determinant Role in Patients Suffering from Periodontitis |
| title_full | Identification of a miRNA Panel with a Potential Determinant Role in Patients Suffering from Periodontitis |
| title_fullStr | Identification of a miRNA Panel with a Potential Determinant Role in Patients Suffering from Periodontitis |
| title_full_unstemmed | Identification of a miRNA Panel with a Potential Determinant Role in Patients Suffering from Periodontitis |
| title_short | Identification of a miRNA Panel with a Potential Determinant Role in Patients Suffering from Periodontitis |
| title_sort | identification of a mirna panel with a potential determinant role in patients suffering from periodontitis |
| topic | periodontitis altered miRNA pattern biological networks |
| url | https://www.mdpi.com/1467-3045/45/3/145 |
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