Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells
Abstract Background Little is known about the differences among adult and foetal equine mesenchymal stem cells (MSCs), and no data exist about their comparative ultrastructural morphology. The aim of this study was to describe and compare characteristics, immune properties, and ultrastructural morph...
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s12917-019-2023-5 |
| _version_ | 1818043215924690944 |
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| author | B. Merlo G. Teti A. Lanci J. Burk E. Mazzotti M. Falconi E. Iacono |
| author_facet | B. Merlo G. Teti A. Lanci J. Burk E. Mazzotti M. Falconi E. Iacono |
| author_sort | B. Merlo |
| collection | DOAJ |
| description | Abstract Background Little is known about the differences among adult and foetal equine mesenchymal stem cells (MSCs), and no data exist about their comparative ultrastructural morphology. The aim of this study was to describe and compare characteristics, immune properties, and ultrastructural morphology of equine adult (bone marrow: BM, and adipose tissue: AT) and foetal adnexa derived (umbilical cord blood: UCB, and Wharton’s jelly: WJ) MSCs. Results No differences were observed in proliferation during the first 3 passages. While migration ability was similar among cells, foetal MSCs showed a higher adhesion ability, forming smaller spheroids after hanging drop culture (P < 0.05). All MSCs differentiated toward adipogenic, chondrogenic and osteogenic lineages, only tenogenic differentiation was less evident for WJ-MSCs. Data obtained by PCR confirmed MHC1 expression and lack of MHC2 expression in all four cell types. Foetal adnexa MSCs were positive for genes specific for anti-inflammatory and angiogenic factors (IL6, IL8, ILβ1) and WJ-MSCs were the only positive for OCT4 pluripotency gene. At immunofluorescence all cells expressed typical mesenchymal markers (α-SMA, N-cadherin), except for BM-MSCs, which did not express N-cadherin. By transmission electron microscopy, it was observed that WJ-MSCs had a higher (P < 0.05) number of microvesicles compared to adult MSCs, and UCB-MSCs showed more microvesicles than BM-MSCs (P < 0.05). AT-MSCs had a lower number of mitochondria than WJ-MSCs (P < 0.05), and mitochondrial area was higher for WJ-MSCs compared to UCB and AT-MSCs (P < 0.05). Conclusions Results demonstrate that MSCs from adult and foetal tissues have different characteristics, and foetal MSCs, particularly WJ derived ones, seem to have some charactestics that warrant further investigation into potential advantages for clinical application. |
| first_indexed | 2024-12-10T08:58:41Z |
| format | Article |
| id | doaj.art-9ab40f0d1f1b406fab32d0a8cf902058 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-10T08:58:41Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-9ab40f0d1f1b406fab32d0a8cf9020582022-12-22T01:55:21ZengBMCBMC Veterinary Research1746-61482019-08-0115111510.1186/s12917-019-2023-5Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cellsB. Merlo0G. Teti1A. Lanci2J. Burk3E. Mazzotti4M. Falconi5E. Iacono6Department of Veterinary Medical Sciences, University of BolognaDepartment for Biomedical and Neuromotor Sciences, University of BolognaDepartment of Veterinary Medical Sciences, University of BolognaSaxon Incubator for Clinical Translation, University of LeipzigDepartment of Comparative Biomedical Sciences, University of TeramoDepartment for Biomedical and Neuromotor Sciences, University of BolognaDepartment of Veterinary Medical Sciences, University of BolognaAbstract Background Little is known about the differences among adult and foetal equine mesenchymal stem cells (MSCs), and no data exist about their comparative ultrastructural morphology. The aim of this study was to describe and compare characteristics, immune properties, and ultrastructural morphology of equine adult (bone marrow: BM, and adipose tissue: AT) and foetal adnexa derived (umbilical cord blood: UCB, and Wharton’s jelly: WJ) MSCs. Results No differences were observed in proliferation during the first 3 passages. While migration ability was similar among cells, foetal MSCs showed a higher adhesion ability, forming smaller spheroids after hanging drop culture (P < 0.05). All MSCs differentiated toward adipogenic, chondrogenic and osteogenic lineages, only tenogenic differentiation was less evident for WJ-MSCs. Data obtained by PCR confirmed MHC1 expression and lack of MHC2 expression in all four cell types. Foetal adnexa MSCs were positive for genes specific for anti-inflammatory and angiogenic factors (IL6, IL8, ILβ1) and WJ-MSCs were the only positive for OCT4 pluripotency gene. At immunofluorescence all cells expressed typical mesenchymal markers (α-SMA, N-cadherin), except for BM-MSCs, which did not express N-cadherin. By transmission electron microscopy, it was observed that WJ-MSCs had a higher (P < 0.05) number of microvesicles compared to adult MSCs, and UCB-MSCs showed more microvesicles than BM-MSCs (P < 0.05). AT-MSCs had a lower number of mitochondria than WJ-MSCs (P < 0.05), and mitochondrial area was higher for WJ-MSCs compared to UCB and AT-MSCs (P < 0.05). Conclusions Results demonstrate that MSCs from adult and foetal tissues have different characteristics, and foetal MSCs, particularly WJ derived ones, seem to have some charactestics that warrant further investigation into potential advantages for clinical application.http://link.springer.com/article/10.1186/s12917-019-2023-5HorseMesenchymal stem cellsAdult, foetal adnexaTransmission electron microscopy |
| spellingShingle | B. Merlo G. Teti A. Lanci J. Burk E. Mazzotti M. Falconi E. Iacono Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells BMC Veterinary Research Horse Mesenchymal stem cells Adult, foetal adnexa Transmission electron microscopy |
| title | Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells |
| title_full | Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells |
| title_fullStr | Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells |
| title_full_unstemmed | Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells |
| title_short | Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells |
| title_sort | comparison between adult and foetal adnexa derived equine post natal mesenchymal stem cells |
| topic | Horse Mesenchymal stem cells Adult, foetal adnexa Transmission electron microscopy |
| url | http://link.springer.com/article/10.1186/s12917-019-2023-5 |
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