Rapid Purification of Fucoxanthin from <i>Phaeodactylum tricornutum</i>
Fucoxanthin is a natural marine xanthophyll and exhibits a broad range of biological activities. In the present study, a simple and efficient two-step method was used to purify fucoxanthin from the diatom, <i>Phaeodactylum tricornutum</i>. The crude pigment extract of fucoxanthin was sep...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/10/3189 |
| _version_ | 1797497516991709184 |
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| author | Xinjie Zhao Liwei Gao Xiangzhong Zhao |
| author_facet | Xinjie Zhao Liwei Gao Xiangzhong Zhao |
| author_sort | Xinjie Zhao |
| collection | DOAJ |
| description | Fucoxanthin is a natural marine xanthophyll and exhibits a broad range of biological activities. In the present study, a simple and efficient two-step method was used to purify fucoxanthin from the diatom, <i>Phaeodactylum tricornutum</i>. The crude pigment extract of fucoxanthin was separated by silica gel column chromatography (SGCC). Then, the fucoxanthin-rich fraction was purified using a hydrophile–lipophile balance (HLB) solid-phase extraction column. The identification and quantification of fucoxanthin were determined by high-performance liquid chromatography (HPLC) and electrospray ionization mass spectrometry (ESI-MS). This two-step method can obtain 92.03% pure fucoxanthin and a 76.67% recovery rate. In addition, <sup>1</sup>H and <sup>13</sup>C NMR spectrums were adopted to confirm the identity of fucoxanthin. Finally, the purified fucoxanthin exhibited strong antioxidant properties in vitro with the effective concentration for 50% of maximal scavenging (EC50) of 1,1-Dihpenyl-2-picrylhydrazyl (DPPH) and 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radicals being 0.14 mg·mL<sup>−1</sup> and 0.05 mg·mL<sup>−1</sup>, respectively. |
| first_indexed | 2024-03-10T03:20:20Z |
| format | Article |
| id | doaj.art-9ab572ccaf6247d1bd0bc06338e68126 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:20:20Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-9ab572ccaf6247d1bd0bc06338e681262023-11-23T12:22:26ZengMDPI AGMolecules1420-30492022-05-012710318910.3390/molecules27103189Rapid Purification of Fucoxanthin from <i>Phaeodactylum tricornutum</i>Xinjie Zhao0Liwei Gao1Xiangzhong Zhao2School of Food Sciences and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaSchool of Food Sciences and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaSchool of Food Sciences and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaFucoxanthin is a natural marine xanthophyll and exhibits a broad range of biological activities. In the present study, a simple and efficient two-step method was used to purify fucoxanthin from the diatom, <i>Phaeodactylum tricornutum</i>. The crude pigment extract of fucoxanthin was separated by silica gel column chromatography (SGCC). Then, the fucoxanthin-rich fraction was purified using a hydrophile–lipophile balance (HLB) solid-phase extraction column. The identification and quantification of fucoxanthin were determined by high-performance liquid chromatography (HPLC) and electrospray ionization mass spectrometry (ESI-MS). This two-step method can obtain 92.03% pure fucoxanthin and a 76.67% recovery rate. In addition, <sup>1</sup>H and <sup>13</sup>C NMR spectrums were adopted to confirm the identity of fucoxanthin. Finally, the purified fucoxanthin exhibited strong antioxidant properties in vitro with the effective concentration for 50% of maximal scavenging (EC50) of 1,1-Dihpenyl-2-picrylhydrazyl (DPPH) and 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radicals being 0.14 mg·mL<sup>−1</sup> and 0.05 mg·mL<sup>−1</sup>, respectively.https://www.mdpi.com/1420-3049/27/10/3189fucoxanthinpurificationSGCCHLB solid-phase extractionHPLCESI-MS |
| spellingShingle | Xinjie Zhao Liwei Gao Xiangzhong Zhao Rapid Purification of Fucoxanthin from <i>Phaeodactylum tricornutum</i> Molecules fucoxanthin purification SGCC HLB solid-phase extraction HPLC ESI-MS |
| title | Rapid Purification of Fucoxanthin from <i>Phaeodactylum tricornutum</i> |
| title_full | Rapid Purification of Fucoxanthin from <i>Phaeodactylum tricornutum</i> |
| title_fullStr | Rapid Purification of Fucoxanthin from <i>Phaeodactylum tricornutum</i> |
| title_full_unstemmed | Rapid Purification of Fucoxanthin from <i>Phaeodactylum tricornutum</i> |
| title_short | Rapid Purification of Fucoxanthin from <i>Phaeodactylum tricornutum</i> |
| title_sort | rapid purification of fucoxanthin from i phaeodactylum tricornutum i |
| topic | fucoxanthin purification SGCC HLB solid-phase extraction HPLC ESI-MS |
| url | https://www.mdpi.com/1420-3049/27/10/3189 |
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