Can Leaves and Stems of <i>Rubus idaeus</i> L. Handle <i>Candida albicans</i> Biofilms?
<i>Candida albicans</i> is an opportunistic pathogen involved in many infections, especially linked to implanted medical devices. Its ability to form biofilms complicates the treatment of these infections as few molecules are active against sessile <i>C. albicans</i>. The aim...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/13/12/477 |
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| author | Clément Bernard Camille Juin Marine Vitry Van Thanh Danh Le Julien Verdon Anne-Solène Toullec Christine Imbert Marion Girardot |
| author_facet | Clément Bernard Camille Juin Marine Vitry Van Thanh Danh Le Julien Verdon Anne-Solène Toullec Christine Imbert Marion Girardot |
| author_sort | Clément Bernard |
| collection | DOAJ |
| description | <i>Candida albicans</i> is an opportunistic pathogen involved in many infections, especially linked to implanted medical devices. Its ability to form biofilms complicates the treatment of these infections as few molecules are active against sessile <i>C. albicans</i>. The aim of this study was to evaluate the potential of leaves, three-month-old and one-year-old stems of <i>Rubus idaeus</i> L. against <i>C. albicans</i> biofilm growth. Extractions with a polarity gradient were carried out on hydroacetonic extracts and followed by fractionation steps. The obtained extracts and fractions were tested for their anti-biofilm growth activity against <i>C. albicans</i> using XTT method. Compounds of active subfractions were identified by LC-MS. The hexane extracts from leaves and stems were the most active against the fungus with IC<sub>50</sub> at 500 and 250 µg/mL. Their bioguided fractionation led to 4 subfractions with IC<sub>50</sub> between 62.5 and 125 µg/mL. Most of the components identified in active subfractions were fatty acids and terpenoïds. |
| first_indexed | 2024-03-10T13:57:20Z |
| format | Article |
| id | doaj.art-9ab6047b35a546fdb762114047abd147 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T13:57:20Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-9ab6047b35a546fdb762114047abd1472023-11-21T01:34:04ZengMDPI AGPharmaceuticals1424-82472020-12-01131247710.3390/ph13120477Can Leaves and Stems of <i>Rubus idaeus</i> L. Handle <i>Candida albicans</i> Biofilms?Clément Bernard0Camille Juin1Marine Vitry2Van Thanh Danh Le3Julien Verdon4Anne-Solène Toullec5Christine Imbert6Marion Girardot7Laboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS 7267, F-86073 Poitiers, FranceLaboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS 7267, F-86073 Poitiers, FranceLaboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS 7267, F-86073 Poitiers, FranceLaboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS 7267, F-86073 Poitiers, FranceLaboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS 7267, F-86073 Poitiers, FranceLaboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS 7267, F-86073 Poitiers, FranceLaboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS 7267, F-86073 Poitiers, FranceLaboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS 7267, F-86073 Poitiers, France<i>Candida albicans</i> is an opportunistic pathogen involved in many infections, especially linked to implanted medical devices. Its ability to form biofilms complicates the treatment of these infections as few molecules are active against sessile <i>C. albicans</i>. The aim of this study was to evaluate the potential of leaves, three-month-old and one-year-old stems of <i>Rubus idaeus</i> L. against <i>C. albicans</i> biofilm growth. Extractions with a polarity gradient were carried out on hydroacetonic extracts and followed by fractionation steps. The obtained extracts and fractions were tested for their anti-biofilm growth activity against <i>C. albicans</i> using XTT method. Compounds of active subfractions were identified by LC-MS. The hexane extracts from leaves and stems were the most active against the fungus with IC<sub>50</sub> at 500 and 250 µg/mL. Their bioguided fractionation led to 4 subfractions with IC<sub>50</sub> between 62.5 and 125 µg/mL. Most of the components identified in active subfractions were fatty acids and terpenoïds.https://www.mdpi.com/1424-8247/13/12/477<i>Rubus idaeus</i> L.raspberry<i>Rosaceae</i><i>Candida albicans</i>anti-biofilmbioguided fractionation |
| spellingShingle | Clément Bernard Camille Juin Marine Vitry Van Thanh Danh Le Julien Verdon Anne-Solène Toullec Christine Imbert Marion Girardot Can Leaves and Stems of <i>Rubus idaeus</i> L. Handle <i>Candida albicans</i> Biofilms? Pharmaceuticals <i>Rubus idaeus</i> L. raspberry <i>Rosaceae</i> <i>Candida albicans</i> anti-biofilm bioguided fractionation |
| title | Can Leaves and Stems of <i>Rubus idaeus</i> L. Handle <i>Candida albicans</i> Biofilms? |
| title_full | Can Leaves and Stems of <i>Rubus idaeus</i> L. Handle <i>Candida albicans</i> Biofilms? |
| title_fullStr | Can Leaves and Stems of <i>Rubus idaeus</i> L. Handle <i>Candida albicans</i> Biofilms? |
| title_full_unstemmed | Can Leaves and Stems of <i>Rubus idaeus</i> L. Handle <i>Candida albicans</i> Biofilms? |
| title_short | Can Leaves and Stems of <i>Rubus idaeus</i> L. Handle <i>Candida albicans</i> Biofilms? |
| title_sort | can leaves and stems of i rubus idaeus i l handle i candida albicans i biofilms |
| topic | <i>Rubus idaeus</i> L. raspberry <i>Rosaceae</i> <i>Candida albicans</i> anti-biofilm bioguided fractionation |
| url | https://www.mdpi.com/1424-8247/13/12/477 |
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