Genetic characterization, antibiogram pattern, and pathogenicity of Clostridium perfringens isolated from broiler chickens with necrotic enteritis
The aims of this investigation were characterization, demonstration of the antibiogram pattern and detection of the pathogenicity of Clostridium perfringens (C. perfringens) strains isolated from broiler chickens in Damietta governorate, Egypt. A total of 357 samples representing 202 intestinal cont...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Diponegoro University, Faculty of Animal and Agricultural Sciences
2022-03-01
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| Series: | Journal of the Indonesian Tropical Animal Agriculture |
| Subjects: | |
| Online Access: | https://ejournal.undip.ac.id/index.php/jitaa/article/view/39152 |
| _version_ | 1830405301394735104 |
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| author | R. D. Eraky W. A. Abd El-Ghany |
| author_facet | R. D. Eraky W. A. Abd El-Ghany |
| author_sort | R. D. Eraky |
| collection | DOAJ |
| description | The aims of this investigation were characterization, demonstration of the antibiogram pattern and detection of the pathogenicity of Clostridium perfringens (C. perfringens) strains isolated from broiler chickens in Damietta governorate, Egypt. A total of 357 samples representing 202 intestinal contents and 155 liver samples from freshly dead broiler chickens were collected from 18 broiler farms. Isolates of C. perfringens were identified morphologically, microscopically, and biochemically. Forty-seven C. perfringens isolates were recovered, which represented 20.3% of the intestinal contents and 3.8% of the liver samples. The toxins and virulence genes of C. perfringens were investigated using polymerase chain reaction. All of the toxigenic C. perfringens strains were type A and carried netB, tpeL, cpe, and plc genes. The in vitro antibiogram of C. perfringens strains revealed 100% sensitivity to gentamycin and levofloxacin and 100% resistance to nalidixic acid and ceftriaxone. The isolated C. perfringens strains were highly pathogenic and induced signs and lesions of necrotic enteritis as well as 43.3% mortalities in 20-day-old chicks. In conclusion, C. perfringens is an important pathogen that affects broiler chickens due to the presence of virulence genes and the pathogenicity in the inoculated birds. |
| first_indexed | 2024-12-20T17:43:17Z |
| format | Article |
| id | doaj.art-9ab77d69f5c0430a9281940c3ab13f7d |
| institution | Directory Open Access Journal |
| issn | 2087-8273 2460-6278 |
| language | English |
| last_indexed | 2024-12-20T17:43:17Z |
| publishDate | 2022-03-01 |
| publisher | Diponegoro University, Faculty of Animal and Agricultural Sciences |
| record_format | Article |
| series | Journal of the Indonesian Tropical Animal Agriculture |
| spelling | doaj.art-9ab77d69f5c0430a9281940c3ab13f7d2022-12-21T19:31:03ZengDiponegoro University, Faculty of Animal and Agricultural SciencesJournal of the Indonesian Tropical Animal Agriculture2087-82732460-62782022-03-0147111610.14710/jitaa.47.1.1-1619969Genetic characterization, antibiogram pattern, and pathogenicity of Clostridium perfringens isolated from broiler chickens with necrotic enteritisR. D. Eraky0W. A. Abd El-Ghany1Department of Bacteriology, Animal Health Research Institute (Damietta branch), EgyptDepartment of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, EgyptThe aims of this investigation were characterization, demonstration of the antibiogram pattern and detection of the pathogenicity of Clostridium perfringens (C. perfringens) strains isolated from broiler chickens in Damietta governorate, Egypt. A total of 357 samples representing 202 intestinal contents and 155 liver samples from freshly dead broiler chickens were collected from 18 broiler farms. Isolates of C. perfringens were identified morphologically, microscopically, and biochemically. Forty-seven C. perfringens isolates were recovered, which represented 20.3% of the intestinal contents and 3.8% of the liver samples. The toxins and virulence genes of C. perfringens were investigated using polymerase chain reaction. All of the toxigenic C. perfringens strains were type A and carried netB, tpeL, cpe, and plc genes. The in vitro antibiogram of C. perfringens strains revealed 100% sensitivity to gentamycin and levofloxacin and 100% resistance to nalidixic acid and ceftriaxone. The isolated C. perfringens strains were highly pathogenic and induced signs and lesions of necrotic enteritis as well as 43.3% mortalities in 20-day-old chicks. In conclusion, C. perfringens is an important pathogen that affects broiler chickens due to the presence of virulence genes and the pathogenicity in the inoculated birds.https://ejournal.undip.ac.id/index.php/jitaa/article/view/39152antibioticsc. perfringenspcrpoultrytoxins |
| spellingShingle | R. D. Eraky W. A. Abd El-Ghany Genetic characterization, antibiogram pattern, and pathogenicity of Clostridium perfringens isolated from broiler chickens with necrotic enteritis Journal of the Indonesian Tropical Animal Agriculture antibiotics c. perfringens pcr poultry toxins |
| title | Genetic characterization, antibiogram pattern, and pathogenicity of Clostridium perfringens isolated from broiler chickens with necrotic enteritis |
| title_full | Genetic characterization, antibiogram pattern, and pathogenicity of Clostridium perfringens isolated from broiler chickens with necrotic enteritis |
| title_fullStr | Genetic characterization, antibiogram pattern, and pathogenicity of Clostridium perfringens isolated from broiler chickens with necrotic enteritis |
| title_full_unstemmed | Genetic characterization, antibiogram pattern, and pathogenicity of Clostridium perfringens isolated from broiler chickens with necrotic enteritis |
| title_short | Genetic characterization, antibiogram pattern, and pathogenicity of Clostridium perfringens isolated from broiler chickens with necrotic enteritis |
| title_sort | genetic characterization antibiogram pattern and pathogenicity of clostridium perfringens isolated from broiler chickens with necrotic enteritis |
| topic | antibiotics c. perfringens pcr poultry toxins |
| url | https://ejournal.undip.ac.id/index.php/jitaa/article/view/39152 |
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