Effects of arsenic exposure on E2 and TRH and their receptor mRNA expressions in rats
BackgroundArsenic can enter the hypothalamus to induce estrogen effect and interfere with the function of the neuroendocrine system. The thyroid endocrine system (hypothalamic-pituitary-thyroid axis) is one of the main endocrine systems, and the mechanism of arsenic-induced thyroid endocrine toxicit...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Editorial Committee of Journal of Environmental and Occupational Medicine
2023-01-01
|
| Series: | 环境与职业医学 |
| Subjects: | |
| Online Access: | http://www.jeom.org/article/cn/10.11836/JEOM22274 |
| _version_ | 1829454633460826112 |
|---|---|
| author | Yuanyan LAI Hongyun LI Xiaowei MA Zhihong JIANG Jun WU |
| author_facet | Yuanyan LAI Hongyun LI Xiaowei MA Zhihong JIANG Jun WU |
| author_sort | Yuanyan LAI |
| collection | DOAJ |
| description | BackgroundArsenic can enter the hypothalamus to induce estrogen effect and interfere with the function of the neuroendocrine system. The thyroid endocrine system (hypothalamic-pituitary-thyroid axis) is one of the main endocrine systems, and the mechanism of arsenic-induced thyroid endocrine toxicity is still unclear. ObjectiveTo investigate the effects of different arsenic exposure levels on estradiol (E2), hypothalamic thyrotropin-releasing hormone (TRH), and their receptor (ERα, ERβ, and TRHR) mRNAs in rats and the possible hypothalamic toxic pathway and mechanism. MethodsSeventy Wister rats were randomly divided a control group (sterile water); low-, medium-, and high-dose arsenic exposure groups [0.8, 4.0, and 20.0 mg·kg−1 sodium arsenite (NaAsO2)]; estrogen receptor inhibitor (ICI182780) intervention + low-, medium-, and high-dose arsenic exposure groups; with 10 animals in each group, half male and half female. Rats in the arsenic exposure groups were exposed to NaAsO2 by drinking water for 19 weeks, and rats in the intervention groups were injected with 0.5 mg·kg−1 ICI182780 via tail vein at week 9, 3 times a week. The levels of E2 and TRH in serum of rats were detected by ELISA. The expression levels of estrogen receptor α (ERα), estrogen receptor β (ERβ), and TRH receptor (TRHR) mRNAs in hypothalamus of rats were detected by real-time PCR (RT-PCR). Results(1) E2 and its receptor mRNA: Compared with the control group, the serum E2 level of female rats was increased in the low-dose and the medium-dose arsenic exposure groups (P<0.05), and the serum E2 level of male rats was increased in the low-dose, the medium-dose, and the high-dose arsenic exposure groups (P<0.05), and the change of female E2 was greater than that of male rats. Compared with the control group, the relative expression levels of ERα mRNA and ERβ mRNA in female rats were increased in the low-dose, the medium-dose, and the high-dose arsenic exposure groups (P<0.05), so were the relative expression levels of ERα mRNA in male rats (P<0.05). (2) TRH and its receptor mRNA: Compared with the control group, the serum TRH level of female rats was increased in the high-dose arsenic group (P<0.05), the relative expression level of TRHR mRNA was increased in the low-dose, the medium-dose, and the high-dose arsenic exposure groups (P<0.05). Results (1) and results (2) suggested that females were more likely than males to have abnormal changes in E2, TRH, and related receptor genes after arsenic exposure. (3) Compared with female rats in the medium-high dose arsenic exposure group, the expressions of TRH and TRHR induced by arsenic exposure were inhibited after the intervention of ICI182780 (P<0.05), suggesting that arsenic in the hypothalamus may have toxic effects on TRH and TRHR by inducing estrogen-like effects. ConclusionArsenic exposure can induce estrogen-like effects in the hypothalamus, interfere with thyroid function, and show dose-dependent and sex differences. E2 and TRH and their receptors may be the toxic pathway of arsenic-related estrogen-like effect. |
| first_indexed | 2024-04-10T07:26:35Z |
| format | Article |
| id | doaj.art-9ab8c7e2de894fd9806b3a6d69251a0f |
| institution | Directory Open Access Journal |
| issn | 2095-9982 |
| language | English |
| last_indexed | 2024-04-10T07:26:35Z |
| publishDate | 2023-01-01 |
| publisher | Editorial Committee of Journal of Environmental and Occupational Medicine |
| record_format | Article |
| series | 环境与职业医学 |
| spelling | doaj.art-9ab8c7e2de894fd9806b3a6d69251a0f2023-02-24T07:11:21ZengEditorial Committee of Journal of Environmental and Occupational Medicine环境与职业医学2095-99822023-01-01401899410.11836/JEOM2227422274Effects of arsenic exposure on E2 and TRH and their receptor mRNA expressions in ratsYuanyan LAI0Hongyun LI1Xiaowei MA2Zhihong JIANG3Jun WU4Department of Labor Health and Environmental Health, School of Public Health, Xinjiang Medical University, Urumqi, Xinjiang 830011, ChinaDepartment of Labor Health and Environmental Health, School of Public Health, Xinjiang Medical University, Urumqi, Xinjiang 830011, ChinaDepartment of Labor Health and Environmental Health, School of Public Health, Xinjiang Medical University, Urumqi, Xinjiang 830011, ChinaDepartment of Labor Health and Environmental Health, School of Public Health, Xinjiang Medical University, Urumqi, Xinjiang 830011, ChinaDepartment of Labor Health and Environmental Health, School of Public Health, Xinjiang Medical University, Urumqi, Xinjiang 830011, ChinaBackgroundArsenic can enter the hypothalamus to induce estrogen effect and interfere with the function of the neuroendocrine system. The thyroid endocrine system (hypothalamic-pituitary-thyroid axis) is one of the main endocrine systems, and the mechanism of arsenic-induced thyroid endocrine toxicity is still unclear. ObjectiveTo investigate the effects of different arsenic exposure levels on estradiol (E2), hypothalamic thyrotropin-releasing hormone (TRH), and their receptor (ERα, ERβ, and TRHR) mRNAs in rats and the possible hypothalamic toxic pathway and mechanism. MethodsSeventy Wister rats were randomly divided a control group (sterile water); low-, medium-, and high-dose arsenic exposure groups [0.8, 4.0, and 20.0 mg·kg−1 sodium arsenite (NaAsO2)]; estrogen receptor inhibitor (ICI182780) intervention + low-, medium-, and high-dose arsenic exposure groups; with 10 animals in each group, half male and half female. Rats in the arsenic exposure groups were exposed to NaAsO2 by drinking water for 19 weeks, and rats in the intervention groups were injected with 0.5 mg·kg−1 ICI182780 via tail vein at week 9, 3 times a week. The levels of E2 and TRH in serum of rats were detected by ELISA. The expression levels of estrogen receptor α (ERα), estrogen receptor β (ERβ), and TRH receptor (TRHR) mRNAs in hypothalamus of rats were detected by real-time PCR (RT-PCR). Results(1) E2 and its receptor mRNA: Compared with the control group, the serum E2 level of female rats was increased in the low-dose and the medium-dose arsenic exposure groups (P<0.05), and the serum E2 level of male rats was increased in the low-dose, the medium-dose, and the high-dose arsenic exposure groups (P<0.05), and the change of female E2 was greater than that of male rats. Compared with the control group, the relative expression levels of ERα mRNA and ERβ mRNA in female rats were increased in the low-dose, the medium-dose, and the high-dose arsenic exposure groups (P<0.05), so were the relative expression levels of ERα mRNA in male rats (P<0.05). (2) TRH and its receptor mRNA: Compared with the control group, the serum TRH level of female rats was increased in the high-dose arsenic group (P<0.05), the relative expression level of TRHR mRNA was increased in the low-dose, the medium-dose, and the high-dose arsenic exposure groups (P<0.05). Results (1) and results (2) suggested that females were more likely than males to have abnormal changes in E2, TRH, and related receptor genes after arsenic exposure. (3) Compared with female rats in the medium-high dose arsenic exposure group, the expressions of TRH and TRHR induced by arsenic exposure were inhibited after the intervention of ICI182780 (P<0.05), suggesting that arsenic in the hypothalamus may have toxic effects on TRH and TRHR by inducing estrogen-like effects. ConclusionArsenic exposure can induce estrogen-like effects in the hypothalamus, interfere with thyroid function, and show dose-dependent and sex differences. E2 and TRH and their receptors may be the toxic pathway of arsenic-related estrogen-like effect.http://www.jeom.org/article/cn/10.11836/JEOM22274arsenichypothalamusestrogen-like effectthyrotropin-releasing hormonethyrotropin-releasing hormone receptor |
| spellingShingle | Yuanyan LAI Hongyun LI Xiaowei MA Zhihong JIANG Jun WU Effects of arsenic exposure on E2 and TRH and their receptor mRNA expressions in rats 环境与职业医学 arsenic hypothalamus estrogen-like effect thyrotropin-releasing hormone thyrotropin-releasing hormone receptor |
| title | Effects of arsenic exposure on E2 and TRH and their receptor mRNA expressions in rats |
| title_full | Effects of arsenic exposure on E2 and TRH and their receptor mRNA expressions in rats |
| title_fullStr | Effects of arsenic exposure on E2 and TRH and their receptor mRNA expressions in rats |
| title_full_unstemmed | Effects of arsenic exposure on E2 and TRH and their receptor mRNA expressions in rats |
| title_short | Effects of arsenic exposure on E2 and TRH and their receptor mRNA expressions in rats |
| title_sort | effects of arsenic exposure on e2 and trh and their receptor mrna expressions in rats |
| topic | arsenic hypothalamus estrogen-like effect thyrotropin-releasing hormone thyrotropin-releasing hormone receptor |
| url | http://www.jeom.org/article/cn/10.11836/JEOM22274 |
| work_keys_str_mv | AT yuanyanlai effectsofarsenicexposureone2andtrhandtheirreceptormrnaexpressionsinrats AT hongyunli effectsofarsenicexposureone2andtrhandtheirreceptormrnaexpressionsinrats AT xiaoweima effectsofarsenicexposureone2andtrhandtheirreceptormrnaexpressionsinrats AT zhihongjiang effectsofarsenicexposureone2andtrhandtheirreceptormrnaexpressionsinrats AT junwu effectsofarsenicexposureone2andtrhandtheirreceptormrnaexpressionsinrats |