Metabolomic Analysis of Aspergillus niger Isolated From the International Space Station Reveals Enhanced Production Levels of the Antioxidant Pyranonigrin A
Secondary metabolite (SM) production in Aspergillus niger JSC-093350089, isolated from the International Space Station (ISS), is reported, along with a comparison to the experimentally established strain ATCC 1015. The analysis revealed enhanced production levels of naphtho-γ-pyrones and therapeutic...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00931/full |
| _version_ | 1818496577312915456 |
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| author | Jillian Romsdahl Adriana Blachowicz Adriana Blachowicz Yi-Ming Chiang Kasthuri Venkateswaran Clay C. C. Wang Clay C. C. Wang |
| author_facet | Jillian Romsdahl Adriana Blachowicz Adriana Blachowicz Yi-Ming Chiang Kasthuri Venkateswaran Clay C. C. Wang Clay C. C. Wang |
| author_sort | Jillian Romsdahl |
| collection | DOAJ |
| description | Secondary metabolite (SM) production in Aspergillus niger JSC-093350089, isolated from the International Space Station (ISS), is reported, along with a comparison to the experimentally established strain ATCC 1015. The analysis revealed enhanced production levels of naphtho-γ-pyrones and therapeutically relevant SMs, including bicoumanigrin A, aurasperones A and B, and the antioxidant pyranonigrin A. Genetic variants that may be responsible for increased SM production levels in JSC-093350089 were identified. These findings include INDELs within the predicted promoter region of flbA, which encodes a developmental regulator that modulates pyranonigrin A production via regulation of Fum21. The pyranonigrin A biosynthetic gene cluster was confirmed in A. niger, which revealed the involvement of a previously undescribed gene, pyrE, in its biosynthesis. UVC sensitivity assays enabled characterization of pyranonigrin A as a UV resistance agent in the ISS isolate. |
| first_indexed | 2024-12-10T18:34:46Z |
| format | Article |
| id | doaj.art-9abf77912e2a4240afcffe4ac363a7e1 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T18:34:46Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9abf77912e2a4240afcffe4ac363a7e12022-12-22T01:37:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-05-011110.3389/fmicb.2020.00931529292Metabolomic Analysis of Aspergillus niger Isolated From the International Space Station Reveals Enhanced Production Levels of the Antioxidant Pyranonigrin AJillian Romsdahl0Adriana Blachowicz1Adriana Blachowicz2Yi-Ming Chiang3Kasthuri Venkateswaran4Clay C. C. Wang5Clay C. C. Wang6Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA, United StatesDepartment of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA, United StatesBiotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United StatesDepartment of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA, United StatesBiotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United StatesDepartment of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA, United StatesDepartment of Chemistry, Dornsife College of Letters, Arts, and Sciences, University of Southern California, Los Angeles, CA, United StatesSecondary metabolite (SM) production in Aspergillus niger JSC-093350089, isolated from the International Space Station (ISS), is reported, along with a comparison to the experimentally established strain ATCC 1015. The analysis revealed enhanced production levels of naphtho-γ-pyrones and therapeutically relevant SMs, including bicoumanigrin A, aurasperones A and B, and the antioxidant pyranonigrin A. Genetic variants that may be responsible for increased SM production levels in JSC-093350089 were identified. These findings include INDELs within the predicted promoter region of flbA, which encodes a developmental regulator that modulates pyranonigrin A production via regulation of Fum21. The pyranonigrin A biosynthetic gene cluster was confirmed in A. niger, which revealed the involvement of a previously undescribed gene, pyrE, in its biosynthesis. UVC sensitivity assays enabled characterization of pyranonigrin A as a UV resistance agent in the ISS isolate.https://www.frontiersin.org/article/10.3389/fmicb.2020.00931/fullsecondary metabolitesAspergillus nigerInternational Space Stationpyranonigrin Aantioxidant |
| spellingShingle | Jillian Romsdahl Adriana Blachowicz Adriana Blachowicz Yi-Ming Chiang Kasthuri Venkateswaran Clay C. C. Wang Clay C. C. Wang Metabolomic Analysis of Aspergillus niger Isolated From the International Space Station Reveals Enhanced Production Levels of the Antioxidant Pyranonigrin A Frontiers in Microbiology secondary metabolites Aspergillus niger International Space Station pyranonigrin A antioxidant |
| title | Metabolomic Analysis of Aspergillus niger Isolated From the International Space Station Reveals Enhanced Production Levels of the Antioxidant Pyranonigrin A |
| title_full | Metabolomic Analysis of Aspergillus niger Isolated From the International Space Station Reveals Enhanced Production Levels of the Antioxidant Pyranonigrin A |
| title_fullStr | Metabolomic Analysis of Aspergillus niger Isolated From the International Space Station Reveals Enhanced Production Levels of the Antioxidant Pyranonigrin A |
| title_full_unstemmed | Metabolomic Analysis of Aspergillus niger Isolated From the International Space Station Reveals Enhanced Production Levels of the Antioxidant Pyranonigrin A |
| title_short | Metabolomic Analysis of Aspergillus niger Isolated From the International Space Station Reveals Enhanced Production Levels of the Antioxidant Pyranonigrin A |
| title_sort | metabolomic analysis of aspergillus niger isolated from the international space station reveals enhanced production levels of the antioxidant pyranonigrin a |
| topic | secondary metabolites Aspergillus niger International Space Station pyranonigrin A antioxidant |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00931/full |
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